L4 (NC_001460) Virus Tagged ORF Clone

CAT#: VC100271

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for L4 gene product [Human adenovirus A], codon optimized for human cell expression, NP_040927



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CNY 11,880.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 100 ul

CNY 600.00


Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
    • 100 ul

CNY 1,050.00

Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC100271 represents NCBI reference of NP_040927 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATGGATCTGGAACCTCAAGAATCCCTGACTGCGCCCACGGCTCCTGCGATCGGAGCAACAGCTGTGA
TGGAGAAAGACAAGTCTCTGCTCATCCCACAGGATGCACCCGTGGAGCAGAATCTGGGGTATGAAACTCC
CCCTGAGGAGTTTGAAGGCTTCCTCCAGATCCAAAAGCAGCCCAATGAACAGAACGCAGGGCTCGAGGAC
CATGACTATCTGAATGAGGGTGATGTGTTGTTTAAACATCTGCAGAGACAGAGCACCATTGTGAGGGACG
CCATTTCAGACCGGTCCTCAATCCCAGTTAGCATCGCAGAACTCTCATGTATCTATGAGCGCAATCTCTT
CAGCCCTCGCGTGCCACCAAAGAGGCAGGCTAACGGGACTTGTGAGCCTAATCCCAGGCTTAATTTCTAC
CCCGTGTTTGCCGTTCCCGAGGCTCTGGCCACTTACCATATCTTCTTTAAGAACCACAAGATTCCGCTCA
GCTGTAGAGCAAATAGGTCTCGCGCGGACGAACTCCTGGCGCTGCGCGCGGGTGCTAGCATCCCTGGAAT
AGTTTCACTTGAGGAGGTGCCTAAAATTTTTGAAGGCCTTGGACGCGACGAAAAACGCGCCGCAAACGCT
CTTCAGAAAGAAAATGAACAGAATCACCACGGGAACTCAGCACTCATAGAACTTGAAGGAGACAACGCCC
GATTGGCAGTACTGAAGAGGAACATCGAGGTTACACACTTTGCATACCCTGCCGTGAACCTCCCACCTAA
GGTAATGTCAGCCGTGATGAACCAGTTGCTCATCAAGAGGGCGCAGCCTATCGATAAAGACGCGAACCTG
CAGGACCCGGAAGCCACAGACGATGGCAAACCCGTCGTTTCAGACGAGCAGCTGACGAAGTGGTTGGGCA
CCGACAATTCTAATGAGCTTCAGCAGCGACGGAAATTGATGATGGCAGCGGTGCTTGTGACAGTGGAGCT
CGAATGTATGCATCGGTTTTTCAGCGATATAACTACACTGCGCAAGATCGAGGAGTGTCTGCATTATACT
TTTCGGCATGGCTACGTTAGGCAGGCGTGCAAGATCTCAAACGTCGAGCTTTCTAATTTGGTCTCTTATA
TGGGTATTCTGCACGAAAATCGACTGGGACAGAATGTCCTTCATTCAACTTTGAGGGATGAAGCCCGCCG
CGACTACGTCAGAGACTGCATTTACCTGTTTCTGCTCCACACATGGCAGACTGGTATGGGAGTTTGGCAG
CAGTGCCTCGAGGAAAAGAACCTTAGGGAACTCAACAAGTTGCTCGATCGCGCTTTGAAGAGTCTGTGGA
CTGGATTCGATGAAAGAACGGTAGCTGCAGAACTCGCAGATATTATTTTCCCCGAGAGGCTGATGATTAC
CCTTCAGAACGGCCTTCCAGACTTCATGTCCCAGAGCATGCTGCATAATTACCGGAGTTTTATCCTGGAG
CGAAGTGGCATGTTGCCTTCAATGTGCTGTGCCTTGCCTAGTGATTTTGTGCCAATTTACTTTCGCGAAT
GCCCGCCCCCTCTGTGGAGCCACTGCTACCTTCTGAGACTGGCTAATTACCTCGCTTATCACTCAGATTT
GATGACTGACAGTAGCGGAGAGGGACTCATGGAGTGTCACTGTCGGTGTAACCTGTGCACCCCACACCGC
TCACTCGTGTGCAACACAGAACTGCTGTCCGAGTCCCAGGTCATCGGTACGTTCGAAATGCAGGGTCCTC
AGAGCGATTCTAACTTTACCACTAATCTGCGGCTTACACCAGGATTGTGGACCAGTGCTTATCTTCGGAA
ATTCGAACCCCAGGACTATCATGCACACAGCATCAATTTTTACGAGGATCAGTCAAAACCTCCGAAGGCT
CCCCTTACTGCTTGCGTGATTACACAAGGAAAGATTCTGGCTCAGCTGCACGCCATAAAGCAAGCCCGCG
AGGAGTTTCTGCTCAAGAAGGGGCATGGCGTGTATCTCGATCCCCAGACCGGAGAAGAACTGAACCTTCC
TTCTCCTCTGTGCGCCACAGCAAGTCCACACTCTCAGCATGTCCCTGAAAGCCGCAAAACCGGCTACTGT
GCCGCGACACTTAAAGAGACAGCAGCGACTGCAGGCAATCTGGGTGGACGGATACTCGGGGAGTCAGGCC
GGGGACGCGGTAGAGGACTGGGAAGAATGGGTGGTGGAGGTGGAGGACAACCAAGAAGAGGATCCCGCGG
AGGCGGAGGTAGGTTTCAAGGAAGAAGCGACAGACGGCAGACAGTTGCGTTCAATCAGGCCCTGAGTAAC
GAAACCCGGTGTGAGCAAATCAGCGAGTCACAACCA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC100271 representing NP_040927
Red=Cloning sites Green=Tags

MMDLEPQESLTAPTAPAIGATAVMEKDKSLLIPQDAPVEQNLGYETPPEEFEGFLQIQKQPNEQNAGLED
HDYLNEGDVLFKHLQRQSTIVRDAISDRSSIPVSIAELSCIYERNLFSPRVPPKRQANGTCEPNPRLNFY
PVFAVPEALATYHIFFKNHKIPLSCRANRSRADELLALRAGASIPGIVSLEEVPKIFEGLGRDEKRAANA
LQKENEQNHHGNSALIELEGDNARLAVLKRNIEVTHFAYPAVNLPPKVMSAVMNQLLIKRAQPIDKDANL
QDPEATDDGKPVVSDEQLTKWLGTDNSNELQQRRKLMMAAVLVTVELECMHRFFSDITTLRKIEECLHYT
FRHGYVRQACKISNVELSNLVSYMGILHENRLGQNVLHSTLRDEARRDYVRDCIYLFLLHTWQTGMGVWQ
QCLEEKNLRELNKLLDRALKSLWTGFDERTVAAELADIIFPERLMITLQNGLPDFMSQSMLHNYRSFILE
RSGMLPSMCCALPSDFVPIYFRECPPPLWSHCYLLRLANYLAYHSDLMTDSSGEGLMECHCRCNLCTPHR
SLVCNTELLSESQVIGTFEMQGPQSDSNFTTNLRLTPGLWTSAYLRKFEPQDYHAHSINFYEDQSKPPKA
PLTACVITQGKILAQLHAIKQAREEFLLKKGHGVYLDPQTGEELNLPSPLCATASPHSQHVPESRKTGYC
AATLKETAATAGNLGGRILGESGRGRGRGLGRMGGGGGGQPRRGSRGGGGRFQGRSDRRQTVAFNQALSN
ETRCEQISESQP

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NC_001460
ORF Size 2346 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NC_001460.1, NP_040927
RefSeq ORF 2346 bp
Locus ID 1460842
MW 87.7 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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