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E2A (NC_001460) Virus Tagged ORF Clone
CAT＃: VC100270
- TrueORF®
Myc-DDK-tagged ORF clone of viral ORF for E2A-L gene product [Human adenovirus A], codon optimized for human cell expression, NP_040926
View other clones from "Virus" (35)
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CNY 5,890.00
Product images
CNY 600.00
CNY 1,050.00
Specifications
| Product Data | |
| Type | Virus Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>The Viral ORF clone VC100270 represents NCBI reference of NP_040926 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s) GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCAGTAACCAACATTCTCAGCGCGAGAGAACACCCGATAGAAGCGCGCAGCCCCCACCTCCCAAAA TGGGGAGGTATTTTCTTGACTCTGAGTCCGAAGAGGAACTGGAAGCCGTTCCACTGCCTCCAAAAAAAAA AGTCAAAAAAAGTATGGCCGCCATACCTCTGAGCCCAGAAAGCGCTGAAGAGGAAGAGGCCGAACCCCCC AGGGCCGTCCTTGGGGTGATGGGCTTCTCTATGCCTCCTGTCCGGATCATGCACCATGCCGACGGCAGTC AATCCTTCCAGAAGATGGAAACAAGACAAGTTCATGTGCTGAAAGCTTCCGCCCAGAATTCTGACGAGAA TGAGAAAAATGTGGTCGTTGTGAGGAACCCAGCCAGTCAGCCCCTGGTTTCCGCTTGGGAGAAAGGAATG GAAGCAATGGCAATGCTGATGGAGAAATATCACGTGGACCATGATGAGAGAGCAACATTTCGGTTTCTTC CTGACCAGGGTTCCGTCTATAAAAAAATCTGCACAACCTGGCTGAATGAGGAAAAAAGAGGTCTCCAGCT CACATTCTCTAGTCAAAAGACTTTTCAGGAACTCATGGGACGGTTCCTGCAAGGCTACATGCAGGCGTAC GCTGGTGTGCAACAGAACAGCTGGGAACCCACCGGGTGTTGCGTATGGGAACACAAGTGTACTGAGAGGG AGGGTGAACTCCGATGCTTGCATGGTATGGAGATGGTAAGGAAGGAACATCTTGTTGAGATGGACGTTAC TTCCGAAAGTGGACAACGCGCCCTTAAAGAGAACCCTTCTAAAGCTAAAGTGGCCCAAAACAGATGGGGC CGAAACGTGGTGCAAATAAAGAACGACGACGCTCGGTGTTGTTTCCATGATGTGGGGTGCGGCAATAACA GTTTTTCTGGCAAGAGCTGCGGACTCTTTTACTCCGAGGGGATGAAAGCACAGATCGCCTTTAGACAGAT CGAGGCCTTCATGCTGGCCGACTATCCACATATGAGGCACGGCCAAAAGCGCCTTCTGATGCCCGTACGG TGCGAGTGTCTGAACAAACAGGATGGACTGCCTAGAATGGGGCGCCAGTTGTGCAAAATCACCCCCTTTA ACCTCTCTAACGTAGACAATATAGACATTAACGAAGTTACCGATCCCGGAGCTCTGGCATCCATCAAATA TCCGTGTCTGTTGGTCTTCCAGTGCGCAAATCCAGTGTACCGGAATGCACGGGGAAACGCAGGTCCAAAC TGTGATTTCAAAATAAGTGCCCCTGATGTGATGGGAGCACTGCAGCTGGTTCGACAGCTCTGGGGTGAAA ATTTTGACGGATCTCCCCCGCGGCTTGTGATTCCCGAGTTTAAATGGCACCAGCGGCTTCAGTACAGAAA TATCAGTCTCCCCACCAATCATGGGGACTGTCGGGAGGAGCCTTTCGACTTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >VC100270 representing NP_040926
Red=Cloning sites Green=Tags MASNQHSQRERTPDRSAQPPPPKMGRYFLDSESEEELEAVPLPPKKKVKKSMAAIPLSPESAEEEEAEPP RAVLGVMGFSMPPVRIMHHADGSQSFQKMETRQVHVLKASAQNSDENEKNVVVVRNPASQPLVSAWEKGM EAMAMLMEKYHVDHDERATFRFLPDQGSVYKKICTTWLNEEKRGLQLTFSSQKTFQELMGRFLQGYMQAY AGVQQNSWEPTGCCVWEHKCTEREGELRCLHGMEMVRKEHLVEMDVTSESGQRALKENPSKAKVAQNRWG RNVVQIKNDDARCCFHDVGCGNNSFSGKSCGLFYSEGMKAQIAFRQIEAFMLADYPHMRHGQKRLLMPVR CECLNKQDGLPRMGRQLCKITPFNLSNVDNIDINEVTDPGALASIKYPCLLVFQCANPVYRNARGNAGPN CDFKISAPDVMGALQLVRQLWGENFDGSPPRLVIPEFKWHQRLQYRNISLPTNHGDCREEPFDF TRTRRLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
| ACCN | NC_001460 |
| ORF Size | 1452 bp |
| OTI Disclaimer | The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NC_001460.1, NP_040926 |
| RefSeq ORF | 1452 bp |
| Locus ID | 1460850 |
| MW | 55.0 kDa |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| VC100254 | Myc-DDK-tagged ORF clone of viral ORF for E1A gene product [Human adenovirus A], codon optimized for human cell expression, NP_040910 |
CNY 3,800.00 |
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| VC100255 | Myc-DDK-tagged ORF clone of viral ORF for E1B gene product [Human adenovirus A], codon optimized for human cell expression, NP_040911 |
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|
| VC100256 | Myc-DDK-tagged ORF clone of viral ORF for E1B gene product [Human adenovirus A], codon optimized for human cell expression, NP_040912 |
CNY 5,890.00 |
|
| VC100257 | Myc-DDK-tagged ORF clone of viral ORF for IX gene product [Human adenovirus A], codon optimized for human cell expression, NP_040913 |
CNY 3,800.00 |
|
| VC100258 | Myc-DDK-tagged ORF clone of viral ORF for IVa2 gene product [Human adenovirus A], codon optimized for human cell expression, NP_040914 |
CNY 5,510.00 |
|
| VC100259 | Myc-DDK-tagged ORF clone of viral ORF for E2B gene product [Human adenovirus A], codon optimized for human cell expression, NP_040915 |
CNY 17,860.00 |
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| VC100260 | Myc-DDK-tagged ORF clone of viral ORF for L1 gene product [Human adenovirus A], codon optimized for human cell expression, NP_040916 |
CNY 3,800.00 |
|
| VC100261 | Myc-DDK-tagged ORF clone of viral ORF for E2B gene product [Human adenovirus A], codon optimized for human cell expression, NP_040917 |
CNY 7,700.00 |
|
| VC100262 | Myc-DDK-tagged ORF clone of viral ORF for L1 gene product [Human adenovirus A], codon optimized for human cell expression, NP_040918 |
CNY 4,470.00 |
|
| VC100263 | Myc-DDK-tagged ORF clone of viral ORF for L1 gene product [Human adenovirus A], codon optimized for human cell expression, NP_040919 |
CNY 7,030.00 |
|
| VC100264 | Myc-DDK-tagged ORF clone of viral ORF for L2 gene product [Human adenovirus A], codon optimized for human cell expression, NP_040920 |
CNY 6,080.00 |
|
| VC100265 | Myc-DDK-tagged ORF clone of viral ORF for L2 gene product [Human adenovirus A], codon optimized for human cell expression, NP_040921 |
CNY 4,090.00 |
|
| VC100266 | Myc-DDK-tagged ORF clone of viral ORF for L2 gene product [Human adenovirus A], codon optimized for human cell expression, NP_040922 |
CNY 3,800.00 |
|
| VC100267 | Myc-DDK-tagged ORF clone of viral ORF for L3 gene product [Human adenovirus A], codon optimized for human cell expression, NP_040923 |
CNY 3,800.00 |
|
| VC100268 | Myc-DDK-tagged ORF clone of viral ORF for L3 gene product [Human adenovirus A], codon optimized for human cell expression, NP_040924 |
CNY 13,870.00 |
|
| VC100269 | Myc-DDK-tagged ORF clone of viral ORF for L3 gene product [Human adenovirus A], codon optimized for human cell expression, NP_040925 |
CNY 3,800.00 |
|
| VC100271 | Myc-DDK-tagged ORF clone of viral ORF for L4 gene product [Human adenovirus A], codon optimized for human cell expression, NP_040927 |
CNY 11,880.00 |
|
| VC100272 | Myc-DDK-tagged ORF clone of viral ORF for L4 gene product [Human adenovirus A], codon optimized for human cell expression, NP_040928 |
CNY 3,800.00 |
|
| VC100273 | Myc-DDK-tagged ORF clone of viral ORF for E3 gene product [Human adenovirus A], codon optimized for human cell expression, NP_040929 |
CNY 3,800.00 |
|
| VC100274 | Myc-DDK-tagged ORF clone of viral ORF for E3 gene product [Human adenovirus A], codon optimized for human cell expression, NP_040930 |
CNY 3,800.00 |
|
| VC100275 | Myc-DDK-tagged ORF clone of viral ORF for E3 gene product [Human adenovirus A], codon optimized for human cell expression, NP_040932 |
CNY 3,800.00 |
|
| VC100276 | Myc-DDK-tagged ORF clone of viral ORF for E3 gene product [Human adenovirus A], codon optimized for human cell expression, NP_040931 |
CNY 3,800.00 |
|
| VC100277 | Myc-DDK-tagged ORF clone of viral ORF for L5 gene product [Human adenovirus A], codon optimized for human cell expression, NP_040933 |
CNY 7,030.00 |
|
| VC100278 | Myc-DDK-tagged ORF clone of viral ORF for E4 gene product [Human adenovirus A], codon optimized for human cell expression, NP_040934 |
CNY 3,800.00 |
|
| VC100279 | Myc-DDK-tagged ORF clone of viral ORF for E4 gene product [Human adenovirus A], codon optimized for human cell expression, NP_040935 |
CNY 3,800.00 |
|
| VC100280 | Myc-DDK-tagged ORF clone of viral ORF for E4 gene product [Human adenovirus A], codon optimized for human cell expression, NP_040936 |
CNY 3,800.00 |
|
| VC100281 | Myc-DDK-tagged ORF clone of viral ORF for L4 gene product [Human adenovirus A], codon optimized for human cell expression, NP_597783 |
CNY 3,800.00 |
|
| VC100282 | Myc-DDK-tagged ORF clone of viral ORF for E4 gene product [Human adenovirus A], codon optimized for human cell expression, NP_597784 |
CNY 3,800.00 |
|
| VC100283 | Myc-DDK-tagged ORF clone of viral ORF for L4 gene product [Human adenovirus A], codon optimized for human cell expression, YP_002640219 |
CNY 3,800.00 |
|
| VC100284 | Myc-DDK-tagged ORF clone of viral ORF for E3 gene product [Human adenovirus A], codon optimized for human cell expression, YP_002640220 |
CNY 3,800.00 |
|
| VC100285 | Myc-DDK-tagged ORF clone of viral ORF for E3 gene product [Human adenovirus A], codon optimized for human cell expression, YP_002640221 |
CNY 3,800.00 |
|
| VC100286 | Myc-DDK-tagged ORF clone of viral ORF for U gene product, partial [Human adenovirus A], codon optimized for human cell expression, YP_002640222 |
CNY 3,800.00 |
|
| VC100287 | Myc-DDK-tagged ORF clone of viral ORF for L2 gene product [Human adenovirus A], codon optimized for human cell expression, YP_002640218 |
CNY 3,800.00 |
|
| VC100288 | Myc-DDK-tagged ORF clone of viral ORF for E4 gene product [Human adenovirus A], codon optimized for human cell expression, YP_002640223 |
CNY 3,800.00 |
|
| VC100289 | Myc-DDK-tagged ORF clone of viral ORF for E4 gene product [Human adenovirus A], codon optimized for human cell expression, YP_002640224 |
CNY 3,800.00 |
