U7 (NC_001664) Virus Tagged ORF Clone

CAT#: VC101357

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for protein UL28 [Human herpesvirus 6], codon optimized for human cell expression, NP_042898



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CNY 18,620.00


货期*
6周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 100 ul

CNY 600.00


Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
    • 100 ul

CNY 1,050.00

Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC101357 represents NCBI reference of NP_042898 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGATCTTGAGGCGAAAGAGATCTCCGGGAACATTCCCAATGAGAACGCGCTCGCCGAACTTTGTCGGC
TGTGCGAGTCTGCTGACCTTTCCCAGATCGAAAATTTTGTGGGACGATATAAGAATTGGTGTCTCTCTAT
TATATGGCCCCGGAACTGTTGGCTGCGCTTCACACCCAGGAAAGACGTGGCCGGCTACACAGAGAAAATG
TTTGAGGATATGGACGATCACTATCAAGGATTTGTCGAGAAACTTTGTCTTCTGGGGACCATTCAAATCC
CATACAGAGATGTTCCCATTCTTATTGGTAGAAGTAAACGAATATTTCTTCACGATCTGGAAACGGATAC
CCTCCACTTCGTTTGCGACAACTTTGAGCAATTTGTAAGATATGGGGTACTCGGAACTAACATCATCACG
TGTGCCGAGCCCGTGTATCGGCATGGCCTCTACGAGGGACCCCGATTTGAGTCCCTGGAGAACCTCATGA
ACAACGATGTGCTTCGAAGTCCCTATACTCTGAATGTTCATGTTAAACTGAACCGCAAAGGGGTCTTGGG
GATTAAAGCAATGCGGAAGCACTACATCGCCATGCTGCGCGAGTTTGACGAGCTGGCGAGGTGTGCTTCT
CTGGACGACGTCGGCCGGTTCGTGTCCCTCAATGTTGGGAGAGATCTGAGACTGGACATGCCAGTGTTCA
AATCACTGACCCTGGGCACCAGAGACTCCGTTTGGACAGGTACCTGTCGACTCGCAAACCTGAAGGAGCA
GGAAGACCTGGTCGAGAAAGTTGTCGTGCTGGGGTATCTTAATGATCACGACTATGAGAGCAAATGTACT
CGCCCCATACTGTGTATTGGCAAATCAGGCAAGATTTACTACTACGACTGGATTGATAATGTGCTGGTCA
AGCTGGGAGACTGTCTTTTGACCTTTCTTCGGGTTGGCTTTGCAAGATTGTTCGCAGATTACGGCTACGA
GAAAATCGGAAAGATCTCCATGCGCTTCGGTCGCATGTCCACCTTGGGAATGTCCGAAACATATCAAAGC
TGTATGTCACTGAAGATTGTCCCTGTCTGTAACGACGAATTTGCCCTGTCCTCATGCGTGCCAACACTGG
ATTTCGACGTGGACGTCCTGAGCGCCGCCTACGGCGATGGTCTGGAAATCAGCAGTCCAGGGCTGCGGTG
CTGCATCGCATGGCCACCTATGTACGCGCTGACCCTCGGCGAATTTTACCATTTCCACACGCATCGGTGG
GTTTCTGCATATGACTGGAGCTCTCTGCTGGACGCAGACGAGTTCATGTCCGCAGTTGGATATGCTCACC
CCATCTATAGAGATCCTAACCCCGATTACGACCCTTATGTCATGCATACTTCAACTGGTAAGACGATAGC
CGTAGATACAGTCACTGAAAAGGTGTATATCATCGCTGAGTCTCTTGTGCAGTTCTTCAATATTGGGCTG
AGACAATTCCCGCCATTCGCCGAGGCTGAACTGGATCCGGAACAGGAGAAAATGTGGTTCGGTGAGACCA
AATGCGGTCGCGAAGAATTCATTCTGCTGCAAAGGAACCTCCCCGCCATGAAGGACTATGTGGCAAAACA
TTCCGGTAAGAGAATACGAGTCGACGCTTTCCAGGACTTTGATTTTTCTTTTTGTAGCCTGTCTGACATT
TACTATCTCACGGGACCAGGGATCTTGGAGAAGATCACTGAGAAAGATTACGCAATCATCGGAACTTGTG
CCCGCAGCCAGGCAGAACCAAATTGCCGAGCAGCAATCGTGATGGGTTCTAACTGTCATATCTATATCTA
TGTCGAGAACAAGATATCCAAAGTATCCAAGTCCCTTCGCACCTTCATCCGGCGAGGATTCGACGAACTG
CTGTATAAAGAGAAATACTCACTGGATTGGAGTGATGACACACTTTTCTACATTAGTGATACTGAGACCG
AAAACCTGAATCGCATGCTTAATGGTGAACTCCCTGTACTGAGATCCAAGCCGCGCCACATGTGTGTCAG
GAAAGACAGACTGGTGAAAGACCGGAGCAAGATTCTCTTTGCTGTCCGCCTTGATGAGGAGGACTCCCCA
ACTGTGAAGTTCATTACCAAATTCCTCACACCGGTGTTCGTGGGCAGATTGCCTGCAACAAACAGATTTG
TAGTGCCCGTTAGCCGCGCTCGGCTGACAAACGGACTGCAGGGTACGGCCGCAGCTCGCTTCGGCATTAA
AGGCCTGCACCCGTCCAGCGATTGTCTGGTATGGAACATCCTTGTGGACTACGAATATGAGACTTACAAA
TATCCTAGCACCTACATCCGGGCCGACCAAATTGCTGATATGGTGAAGGACCTGAAGTTCATGGACAACT
TTAACGAGAAGTGGCAGTGCATTACCAAACTTGCCTTCCTGGGCCTGTACGCAGGAGCCAGCCTCTTTAA
CTTTACCTCAAAGCCCACTCTCGGCTATTGGTGCAGATACCTTTCCGAGTACGCCTCAATGTTGCTCTTC
CAGTTCGAGAGTAAGCTGAAAGAACTCACAAAGGAGAGTACTAGGCAACTGGGCGGGTACAATCTGTGCC
ACTGGGGACAGGAACTCAAGGATTGCCTGGAAAATAAGTCTGACGTTTTTTTCCGCTACGATTTCTTCGA
GCGGATCGAGAGTTGCCTTATAGAACACTTTATGCTTCTTTGTGGGTGTGTGGAGTGCCGGCGGATGTTC
ATCATGTATAATAAGAGGGGAAGGAAATTCGACTTTGGACACAGTGTGAGAATTCAATGTTTTCCCATGA
TTGGATCCATTCGGCTTCCCGCCTTTCTGCACCTGGGTGAGCCATATGACGTATCCCTTAGCTCACTCAT
AGCCAAAGACCTGGGTCTGAGCATGATTGAAGGACAGATTGAGCTGTCTCGCCTGCCCATTTCCCTTCAG
ATCTCCGTGACTCCAGACAAGAAGGCCCTCCTGACATTTCTCACCAATATCGTCTTCATTGTGTTCGTTG
TGAACACCTTGTACAGAGTGATCAACGCTGAACTCGACATTTACTACGATCTTTTTACAGAGGAAGTCGG
GAAGTTGTGCGTCGCCATGGAGGAGGAAATGAAACTGGGCCGGAATGGATGCCTCGGGGACCTGTGCTAC
TTTTCCCCAATGAAACAGATGAAAGAGATCGTCCGATGCCCCGGAGAAAAAAGCCAATTTATTCTCAAGT
GTTGGGAGGCTCTGAGAATTGGATTCAGCGTTCCTGCCTACAAAGACTACGACGAGACACCGTTCATGGA
GATGTTCTTTATGCACCACCTTCACATCAAGCGCTTCCACGAGGACAACGATCGCGACCTGGTATCTTGT
GACAACCTGATTCCCGGATTTTTCATAGTGAATACCGACGGAGAGAATTTTCTGCAGAGGCTTCAGAGAG
TAGTCCTGCCTGTGGTGGAGGATTATCTGACCAACACCAGATGCATCAACGGGACGATGGCCTTTTTCTT
TAGTGGCTTGAAGTACTTCGGATCCGGCAACCATCGCGGTTTCCAGATCAGCCCTGAGAAGGATGTGCGG
GCCATAGCTTATAAATTGGGTAGTCTCGACGTTCTCCGCGATGACTATAAGTATTATGAGTATACCCCAC
CCGACTGTCCCGGCGAACTGAACGGTCACGGTGGCGATGAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC101357 representing NP_042898
Red=Cloning sites Green=Tags

MDLEAKEISGNIPNENALAELCRLCESADLSQIENFVGRYKNWCLSIIWPRNCWLRFTPRKDVAGYTEKM
FEDMDDHYQGFVEKLCLLGTIQIPYRDVPILIGRSKRIFLHDLETDTLHFVCDNFEQFVRYGVLGTNIIT
CAEPVYRHGLYEGPRFESLENLMNNDVLRSPYTLNVHVKLNRKGVLGIKAMRKHYIAMLREFDELARCAS
LDDVGRFVSLNVGRDLRLDMPVFKSLTLGTRDSVWTGTCRLANLKEQEDLVEKVVVLGYLNDHDYESKCT
RPILCIGKSGKIYYYDWIDNVLVKLGDCLLTFLRVGFARLFADYGYEKIGKISMRFGRMSTLGMSETYQS
CMSLKIVPVCNDEFALSSCVPTLDFDVDVLSAAYGDGLEISSPGLRCCIAWPPMYALTLGEFYHFHTHRW
VSAYDWSSLLDADEFMSAVGYAHPIYRDPNPDYDPYVMHTSTGKTIAVDTVTEKVYIIAESLVQFFNIGL
RQFPPFAEAELDPEQEKMWFGETKCGREEFILLQRNLPAMKDYVAKHSGKRIRVDAFQDFDFSFCSLSDI
YYLTGPGILEKITEKDYAIIGTCARSQAEPNCRAAIVMGSNCHIYIYVENKISKVSKSLRTFIRRGFDEL
LYKEKYSLDWSDDTLFYISDTETENLNRMLNGELPVLRSKPRHMCVRKDRLVKDRSKILFAVRLDEEDSP
TVKFITKFLTPVFVGRLPATNRFVVPVSRARLTNGLQGTAAARFGIKGLHPSSDCLVWNILVDYEYETYK
YPSTYIRADQIADMVKDLKFMDNFNEKWQCITKLAFLGLYAGASLFNFTSKPTLGYWCRYLSEYASMLLF
QFESKLKELTKESTRQLGGYNLCHWGQELKDCLENKSDVFFRYDFFERIESCLIEHFMLLCGCVECRRMF
IMYNKRGRKFDFGHSVRIQCFPMIGSIRLPAFLHLGEPYDVSLSSLIAKDLGLSMIEGQIELSRLPISLQ
ISVTPDKKALLTFLTNIVFIVFVVNTLYRVINAELDIYYDLFTEEVGKLCVAMEEEMKLGRNGCLGDLCY
FSPMKQMKEIVRCPGEKSQFILKCWEALRIGFSVPAYKDYDETPFMEMFFMHHLHIKRFHEDNDRDLVSC
DNLIPGFFIVNTDGENFLQRLQRVVLPVVEDYLTNTRCINGTMAFFFSGLKYFGSGNHRGFQISPEKDVR
AIAYKLGSLDVLRDDYKYYEYTPPDCPGELNGHGGDE

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NC_001664
ORF Size 3681 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NC_001664.2, NP_042898
RefSeq ORF 3681 bp
Locus ID 1487887
MW 141.5 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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