U43 (NC_001664) Virus Tagged ORF Clone

CAT#: VC101391

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for helicase-primase primase subunit [Human herpesvirus 6], codon optimized for human cell expression, NP_042936



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CNY 13,020.00


货期*
6周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 100 ul

CNY 600.00


Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
    • 100 ul

CNY 1,050.00

Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC101391 represents NCBI reference of NP_042936 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACAATCGTTGTTTTTGCCACCGAGTACGATGCTGCTAATGTCATCTTTAGCATACTCTGCCGGTCCC
CGTCCGAGCATCTGATCTTCCCAATTATAGTGAAATACAAACCAAGTAATAATGTCAGCTTCTGCCTGCA
GACTCAGAAGTGTAAGAACAGCAAAAGGATTGATACCGTGTTCGTCTGCCACGCCGAAAAGCTGAATCTC
TCACACTACATACAGACAGCATCACCAATCAAAGCCGAAGATGTCGCCAACAGTTTGAACGATAAAGAAA
CCGAGTCACTGTACGTGGACATGATCCTCTCCCAAACCGGGAAAGAACGCGAGGATGTAGAGTTTAAATA
TATGGCATACTTTCACAAAAGTCTGATCATCAAATACCTGACCGGAAAGTTTCTGCTGCCTACATCTCCA
TTCTGGTTCCTCTCTACTTACGGGCAGACAGAGGGCATGCTGCTGTTGACCATGTTCTACTACCTGTTCG
AGGAGCAGAAATCAACCATTACGACGACTAAGAATTATGTTCAATGTTTCACCGAGAACACAGGCAACAT
GGTCTTCACATACTCTAGCATGTCCGAGTTTATCAATATAACTCTGAAAAGCAAGTTTAGGAAACTCTTT
GCAGACTTTAGTACCTACGCCAGACAGAAAAACCTCCGGGACAAGGAGGAATTTAAGCATCTGGATACAC
AGATTAACCTCTTCCGCAAGAGCTCCCATCTGACAAATACCTTTCGAGTGCACTATATTTATATAGCCTA
CAATACCGCTCTGGAGACCACCAAATTTGTGAATTACTGCAACCTCACCTCTTACGATTCTAATCTCCCT
ATTGGTCAGCAATGCCAGCGGAACCTGCATATCCTCGGTAATTCCCTCCACGAGAATCTGCTGTGTATCA
TGAAACAGTATTTCAACGCAGATTGTTACTTCAAGACTTACATCGACATCAAGAGGCTGAAGAATCCTAA
CCTCAATGTCACCGAATACGAATATGCCCTGGTGTCCAAAAAGAAAACTATCCAAGCCCTCACCAGCGAG
CAAATCACTCGAGCAATAGCTAAATGTAACAAGAATGGGGAGGGTCTGTTTTCTCCAGTAAAGCCTGGGC
TCCAGGGGCTGCTGGAGATATCTGCATCCGACAGATACGTTCAGATCCAGGACAAGAGAATCTACCGCCG
CCAACACCTGCACAAGGACTATCACCGCCCATTCCCCGTGTTTAGAGTGCAGTTGCTGCACAAGAACATC
TTTTGCTTTGGCAACTCAGAGGATTGGTATGAAAACATGGGCTTCAATAGAATATTGCAATATCTCCCGG
ATGAATATATATCCGACGAGGCCCTGACGAGAGCCATCTGGCTGCAGGACACCCACTTCCTGTGTGATGA
TGTGGAAAAGCAATTCTACACCACGCGGCACGAAATCTTCAACGAGAGGATCCCCGTGACTAACTACATC
GGCGACCTCGACCTGCCCCTTCAGGACACCGCAACCATAACCGAAGAGACATTTTTTAGCATGTGTCGCC
TGATTCGCCTCACCCTCATCAATGCCTGGAAGAAGATCTTCCCTTCAATCGATACAGACACTCATCCTAT
CTTTTTCTTTAAGACACAGTGCGACACGACCAACGACACTCTCGACTATACCGAAGACCCCACAGAAATC
AAGCAGTTTTGCGTATGTAGAAAGAAAATCGGGCTTCGAATCAGCATCCCATTGCCTAATGGAACAGCTA
TTGCCGGCGGGGAACCTTTGAAACAGCTTTCCAAGATTCTTAACCATGTAATGTGCCTGGACCAGGAACT
CAGCCAGATACTCAATTCTATCACCTTCCCCGGCGAATGCTTCGACATTGGCATCTATCACACAGGACAC
TGCATACGGATTGGATATATGTACAAAACTGACATGGATAAGGGGAAGATGCTCCATGGACGGCTTACAC
CAATCTTTATTGTCCCCGAAGGCTATAGGAACAGTTGCAAGACCTTTATACAAATGCAGATGGATCTCAA
CAATCTCCTGCATCATGGCACAAAAAAGGCCCCCATTGAGGAGCTGATCTACAATATTACAGATAAGGGG
TGCCCAAAGGAGAATCTTAGTTTTATGGACCTGAAGAGTAGGCAGTTGTGGAACAAGGTGAACATAGCCA
CCGAAACACTCATAACGAAATACCTGAACACTCATGGCTTCAATAATAACGCCACAAGCGCTGACGACAG
CTTGCTGTCCTTCATCCGCCTCATAGGCTGGCCTATAATAAAAACACAACTCATCACACACTACGAAACA
AGAATCGCCCAGCAGTTCTCTCAGGTTACTTTTATAAAAATTGATTCCAAGAATCTGCAAATTAAAAAGA
CCCAGTTTGGGCGCGTAAGCGACTTTTCCTGCCTCAATCGCCAACACAGAGGAAATCGAGATAATGTTCT
GGTCTATATTCAGCTGAAGGCGGACGGAAACCGGCTGATTCTGATTCTCTGGAGCACCTGCTTTGCAACT
AAATGCCAGTCTAACTCCAAGCAAGTTCATTGCAGCATAGCGCTGGAGCAGCTGAAGAAT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC101391 representing NP_042936
Red=Cloning sites Green=Tags

MTIVVFATEYDAANVIFSILCRSPSEHLIFPIIVKYKPSNNVSFCLQTQKCKNSKRIDTVFVCHAEKLNL
SHYIQTASPIKAEDVANSLNDKETESLYVDMILSQTGKEREDVEFKYMAYFHKSLIIKYLTGKFLLPTSP
FWFLSTYGQTEGMLLLTMFYYLFEEQKSTITTTKNYVQCFTENTGNMVFTYSSMSEFINITLKSKFRKLF
ADFSTYARQKNLRDKEEFKHLDTQINLFRKSSHLTNTFRVHYIYIAYNTALETTKFVNYCNLTSYDSNLP
IGQQCQRNLHILGNSLHENLLCIMKQYFNADCYFKTYIDIKRLKNPNLNVTEYEYALVSKKKTIQALTSE
QITRAIAKCNKNGEGLFSPVKPGLQGLLEISASDRYVQIQDKRIYRRQHLHKDYHRPFPVFRVQLLHKNI
FCFGNSEDWYENMGFNRILQYLPDEYISDEALTRAIWLQDTHFLCDDVEKQFYTTRHEIFNERIPVTNYI
GDLDLPLQDTATITEETFFSMCRLIRLTLINAWKKIFPSIDTDTHPIFFFKTQCDTTNDTLDYTEDPTEI
KQFCVCRKKIGLRISIPLPNGTAIAGGEPLKQLSKILNHVMCLDQELSQILNSITFPGECFDIGIYHTGH
CIRIGYMYKTDMDKGKMLHGRLTPIFIVPEGYRNSCKTFIQMQMDLNNLLHHGTKKAPIEELIYNITDKG
CPKENLSFMDLKSRQLWNKVNIATETLITKYLNTHGFNNNATSADDSLLSFIRLIGWPIIKTQLITHYET
RIAQQFSQVTFIKIDSKNLQIKKTQFGRVSDFSCLNRQHRGNRDNVLVYIQLKADGNRLILILWSTCFAT
KCQSNSKQVHCSIALEQLKN

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NC_001664
ORF Size 2580 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NC_001664.2, NP_042936
RefSeq ORF 2580 bp
Locus ID 1487921
MW 100.1 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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