U11 (NC_001664) Virus Tagged ORF Clone

CAT#: VC101359

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for tegument protein pp150 [Human herpesvirus 6], codon optimized for human cell expression, NP_042902



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CNY 13,210.00


货期*
6周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 100 ul

CNY 600.00


Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
    • 100 ul

CNY 1,050.00

Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC101359 represents NCBI reference of NP_042902 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGACCTGCAGCGGCATCCAATCCCCTTTGCATGGCTGGATAGAGACAAGGTTGAGAGGTTGACTGACT
TTCTTTCAAATCTGGAGCGGCTCGACAATGTCGATCTGAGGGAACACCCCCACGTTACCAACTCCTGCGT
GGTACGGGAAGGTGACGACGTAGATGATCTGAAAACCCTGTATAACTTGCTGGTGCTCTGGCTTATGTAT
CACTATGTGCTTTCCAAGAGAAAGCCAGACTATAACGCAATTTGGCAGGATATCACCAAACTGCAGTCCG
TGGTCAACGAGTACTTGAACTCAAAAGGTCTCAACAAGGGCATATTTGAGAATATGTTTACTAATAAGGA
GAAATTTGAGAGCCAATTTTCTGATATCAATCGAGCACTTCTGCGGCTCGGGAATTTTATTAAATGGGGA
AGTAACGTCGCTATCGACACACCTTATGTGAATCTGACTGCTGAGGATTCTTCCGAAATCGAGAATAACC
TTCAGGACGCCGAAAAAAATATGTTGTGGTATACCGTATATAATATCAATGATCCTTGGGACGAAAATGG
GTACCTCATAACATCCATAAACAAACTCATCTATTTGGGCAAACTCTTCCTGGCTCTTACTCAGAGTTGG
AGTAAGCTGGAGAAGGTGGCCATGAGTCAGATAGTGATCACCCAGAATCACCTGTCAGGGCATCTTCGGA
GACACGACAATTTCAACATCGTGTATTCACATCGGGTGCTTCAGACACCTCTGACCGGTCAGCGAGTAGA
GTCCTTTCTTAAAATCATTACGTCCGACTACGATATTATCAAGAGCTCTCTGGAGAGTCACTCTGCGTCC
AAGGCCTTTTCTATGTCAGAAATCGGACCCAACTCTCTGATGGACTTTGTCCCTCTTAGAGGCGATATCC
ACAGCAACTTGACATTGCCATCCATGTCCATAGACACTAAGAAAAGCTCCCTGGACCCTGCCCGGTTGAA
GAAATCCAATAGCCGATCCCTGGACTCTTTTCTTAGGATGCAGCGACAGCCTAAATTTCTCGAACTGGAT
TCCGTCGACAACGCCGGTGAGAAAATTTTGCTGAAGGAAGCCACACTGGGCGGCGAGAATGTGAAGGCTA
CTACCCCTGCCTCCAGTGTCTCCCTGATGTCCGGCGTAGAATCTCCCAGTTCCTTTACCAGCACTAACCT
GGACCTGCCCCTCTCTTCATTTACATCCACTAACCTGGACCTGAGAGACAAAAGCCATGGCAACTACAAG
ATTGGCCCTTCAGGGATACTGGACTTTAATGTGAAATTTCCTCCAAACGCCCAGCTGAATACCAATGGGG
TGGATCTCCTGCAGGACAAAACTAGCATTGGGTCACCTAGTTCAGGAATCACAGACGTCGTCAATGGCTT
CGCGAACCTTAATCTGCATCAGAACAAATCTAATGTCTCTCCACCATGGTCAAGAAATACAGCAGCAAAC
GCTGACTTTCTTGATCCAGTCCATCGGTTCGTGCCTGAACAGACTGGCACCCCTTTCGTACTGAACAATA
GCGACGTGGCCGGCAGTGAAGCGAAGCATACCACGTACTCTACTGAGACAGGAGTGTCTCCTCGGAATGT
CTTCCTCATCAAAGACCTTAGAGGGAAGGACGGCTTTCGGAAACAGAAACAGAGCGACATCCCTAAATCC
TTGACCAAGGAGAGGAACGACAAGGCCATCATGCACTCCCGAGAGGTTACTGGCGACAGCGGGGATGCGA
CCGAAACCGTGGGCGCACGAAATAGCCCTGCGCTCCGCAAGATCAAGCAGGCCAATGACTTTTTTGCTGG
ACTCAACAAGAAAAATGATAGGGACGTATTGCGGGGCGGCAAAGGTAACTCCAAGGACCTGCACAGCGGC
GGAAATGCTAAAAAAAAGGAGATGTCCGGCAAATTTAACGATGACAAGGAAATGACACGGAACGGCCAGG
AGCCTTCCAGAAGCCTGATGGGCGACGCCAGGAACGCCGGCGATGAGCAGTACATCCAGGCCGGCCTGGG
ACAGAGAGTGAACAACCTGCTGTCTCAGTTTACAAACTTGATCAGCCTGGGAGAGAAGGGCATAGAAGAC
ATCCTGCAGAACCAGCGGGGCACTGAGCTCAAACTGGCAACCGAGAATAAGTCCGGCAGGGAGAGCGAAG
AGGCCAATGTCGAAAAAATCCTGGAAGTGTCCAACCCCCAGGATATGTTCAAAAACTTTAGGCTGCAGAA
CGACCTGGATTCTGTACAGAGCCCCTTTCGGCTTCCGGATGCGGACCTGTCAAGAGAACTGGACTCCGCC
AGCTTCAAAGACGCACTCGACCTTAAACTGCCTGGCAACGGGGAACGAGAGATCGACCTGGCCTTGGAGA
AGGTGAAGGTTGGCGAGACCGAGACTTCAGATCTTAAGGTAGGGCAGGACGAGAGCTTCGTTCCTGCACA
GCTGATGAAGGTGGAGACCCCCGAAGAGAAAGATGACATTATCGAACAGATGGTGCTGCGGATTAGGCAG
GATGGCGAGACTGATGAGAACACGGTTTCCGGCCCTGGGGTGGCCGAGTCTCTTGACATTGAGGCGAAAG
GAGAAAGTGCGATAGCAAGC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC101359 representing NP_042902
Red=Cloning sites Green=Tags

MDLQRHPIPFAWLDRDKVERLTDFLSNLERLDNVDLREHPHVTNSCVVREGDDVDDLKTLYNLLVLWLMY
HYVLSKRKPDYNAIWQDITKLQSVVNEYLNSKGLNKGIFENMFTNKEKFESQFSDINRALLRLGNFIKWG
SNVAIDTPYVNLTAEDSSEIENNLQDAEKNMLWYTVYNINDPWDENGYLITSINKLIYLGKLFLALTQSW
SKLEKVAMSQIVITQNHLSGHLRRHDNFNIVYSHRVLQTPLTGQRVESFLKIITSDYDIIKSSLESHSAS
KAFSMSEIGPNSLMDFVPLRGDIHSNLTLPSMSIDTKKSSLDPARLKKSNSRSLDSFLRMQRQPKFLELD
SVDNAGEKILLKEATLGGENVKATTPASSVSLMSGVESPSSFTSTNLDLPLSSFTSTNLDLRDKSHGNYK
IGPSGILDFNVKFPPNAQLNTNGVDLLQDKTSIGSPSSGITDVVNGFANLNLHQNKSNVSPPWSRNTAAN
ADFLDPVHRFVPEQTGTPFVLNNSDVAGSEAKHTTYSTETGVSPRNVFLIKDLRGKDGFRKQKQSDIPKS
LTKERNDKAIMHSREVTGDSGDATETVGARNSPALRKIKQANDFFAGLNKKNDRDVLRGGKGNSKDLHSG
GNAKKKEMSGKFNDDKEMTRNGQEPSRSLMGDARNAGDEQYIQAGLGQRVNNLLSQFTNLISLGEKGIED
ILQNQRGTELKLATENKSGRESEEANVEKILEVSNPQDMFKNFRLQNDLDSVQSPFRLPDADLSRELDSA
SFKDALDLKLPGNGEREIDLALEKVKVGETETSDLKVGQDESFVPAQLMKVETPEEKDDIIEQMVLRIRQ
DGETDENTVSGPGVAESLDIEAKGESAIAS

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NC_001664
ORF Size 2610 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NC_001664.2, NP_042902
RefSeq ORF 2610 bp
Locus ID 1487891
MW 97.1 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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