U57 (NC_001664) Virus Tagged ORF Clone

CAT#: VC101407

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for major capsid protein [Human herpesvirus 6], codon optimized for human cell expression, NP_042950



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CNY 20,330.00


货期*
7周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 100 ul

CNY 600.00


Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
    • 100 ul

CNY 1,050.00

Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC101407 represents NCBI reference of NP_042950 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAAAACTGGCAGGCGACAGAGATCCTTCCCAAAATCGAGGCCCCTCTCAATATCTTCAACGACATAA
AAACATATACAGCCGAGCAGCTCTTCGATAATCTTCGCATATATTTCGGCGATGACCCTAGCCGGTATAA
CATATCTTTCGAGGCCCTCCTGGGGATCTATTGTAATAAGATCGAGTGGATTAATTTCTTTACGACTCCC
ATTGCCGTGGCAGCGAACGTGATCCGATTCAATGACGTCTCCAGGATGACCTTGGGAAAGGTGCTGTTCT
TTATCCAACTGCCCAGGGTGGCCACAGGCAATGATGTGACAGCTAGCAAGGAAACCACCATCATGGTGGC
CAAGCACTCAGAAAAACACCCAATTAATATATCCTTTGACCTTAGTGCCGCATGTCTGGAGCACCTGGAG
AACACTTTCAAGAACACTGTCATCGATCAGATCCTGAACATTAATGCGCTGCACACTGTCTTGCGCTCCC
TCAAAAACTCCGCAGACTCCCTGGAGCGGGGCCTGATCCATGCATTCATGCAAACACTCCTGAGGAAAAG
TCCCCCCCAGTTCATCGTTCTTACAATGAATGAAAACAAGGTTCATAACAAACAGGCCCTGAGTAGAGTT
CAACGCTCTAATATGTTTCAAAGCTTGAAAAATAGGCTGCTGACAAGTCTGTTCTTTCTTAACCGCAATA
ATAACATCTCATATATTTACCGGATTTTGAACGATATGATGGAGTCAGTCACCGAGTCAATCCTTAACGA
TACCAACAACTATACCTCAAAGGAAAATGTGCCTCTCGATGGGGTGCTGCTCGGACCCATCGGATCTATT
CAGAAGCTGACAAGTATTCTTTCCCAATACATTTCAACACAGGTGGTGTCCGCGCCAATCAGTTATGGCC
ACTTCATAATGGGCAAGGAGAATGCAGTGACGGCAATTGCTTATCGGGCTATAATGGCTGACTTTACACA
GTTCACTGTGAATGCAGGAACAGAACAGCAAGACACCAATAACAAGTCCGAAATCTTCGATAAAAGCCGG
GCCTACGCGGACCTGAAGCTGAACACCCTGAAGTTGGGCGATAAACTGGTCGCTTTTGACCATTTGCATA
AGGTGTATAAAAACACGGATGTAAACGATCCACTCGAACAGTCCTTGCAGCTGACTTTCTTTTTCCCTCT
CGGCATATACATACCTAGTGAGACAGGGTTCTCCACTATGGAGACGCGCGTCAAGCTGAACGATACAATG
GAGAATAACCTTCCGACGAGCGTGTTCTTTCATAACAAGGATCAGGTGGTACAAAGGATCGACTTCGCAG
ATATACTGCCTAGCGTGTGTCACCCAATTGTGCACGATTCAACCATTGTAGAGAGGCTGATGAAGAGTGA
GCCCCTCCCAACAGGCCACAGGTTCAGCCAGCTGTGCCAGCTGAAGATCACTAGGGAAAATCCTGCCCGC
ATTCTCCAAACTCTGTACAATCTCTATGAGTCACGGCAGGAGGTTCCTAAAAACACAAACGTCCTGAAGA
ACGAGCTCAATATAGAGGACTTCTATAAGCCTGACAACCCCACCCTGCCCACTGAGCGACATCCATTCTT
CGATCTTACTTACATCCAAAAGAATCGGGCTACCGAGGTGCTGTGTACTCCACGAATCATGATTGGAAAC
ATCCCACTCCCTCTGGCCCCAGTGAGCTTCCATGAAGCCCGCACCAACCAAATCTTGGAGCACGCAAAGA
CAAATTGCCAGAAGTATGACTTCACACTCAAGATCGTGACCGAGAGCCTGACTAGCGGCTCCTATCCCGA
GCTGGCATACGTGATCGAGACGCTGGTCCATGGCAACAAGCACGCCTTCATGATACTGAAGCAGGTGATC
AGCCAATGCATAAGCTACTGGTTTAATATGAAACACATCCTCCTGTTCTGCAACTCCTTCGAAATGATCA
TGCTGATCTCTAATCACATGGGGGATGAGCTTATCCCGGGCGCGGCTTTCGCCCACTATAGAAACCTCGT
GAGCCTCATTAGACTGGTGAAGCGGACTATCAGCATAAGCAATTTGAATGAGCAGCTCTGCGGCGAACCC
TTGGTGAACTTTGCGAACGCTCTCTTTGATGGCCGATTGTTTTGCCCTTTCGTCCATACTATGCCGCGCA
ATGACACTAATGCTAAAATCACTGCAGATGATACCCCTCTGACCCAGAATACCGTGCGGGTTCGCAACTA
CGAGATTTCTGACGTTCAAAGAATGAACCTTATCGATTCCTCTGTGGTCTTTACTGATAACGACCGACCT
TCCAACGAGACCACGATTCTGTCTAAAATTTTCTACTTCTGCGTGCTTCCAGCTCTCTCCAACAACAAGG
CATGTGGGGCTGGTGTCAACGTCAAGGAGCTGGTACTCGACCTTTTCTACACCGAGCCTTTCATTAGTCC
CGACGACTACTTTCAGGAAAATCCAATCACAAGTGACGTGCTGATGTCACTGATTAGAGAGGGGATGGGT
CCAGGTTATACCGTTGCAAATACCAGCTGTATTGCCAAACAGCTGTTCAAGTCTCTGATCTACATTAACG
AGAATACAAAGATTCTGGAGGTAGAAGTGTCCCTGGATCCAGCCCAGCGCCATGGAAACAGCGTGCACTT
TCAAAGTTTGCAGCACATACTGTACAATGGCCTCTGCCTTATCAGCCCTATAACCACCTTGCGAAGGTAT
TACCAGCCCATACCCTTCCATAGATTTTTTTCTGACCCTGGGATCTGCGGGACCATGAATGCGGACATTC
AGGTTTTTCTCAATACTTTTCCACATTGTCAGCGCAATGACGGAGGTTTCCCGCTGCCACCTCCCCTGGC
ACTCGAGTTTTATAATTGGCAGAGAACGCCGTTTAGCGTGTACAGCGCTTTTTGCCCGAACTCACTTCTG
TCTATCATGACACTGGCAGCTATGCATAGTAAGCTGAGTCCGGTGGCAATCGCCATCCAATCCAAAAATA
AGATCCATCCTGGATTTGCCGCTACCCTGGTAAGGACCGATAATTTCGACGTAGAGTGTCTGCTCTACAG
TTCACGCGCCGCCACTTCTATAATCCTGGATGACCCTACCGTGACCGCCGAAGCTAAGGACATCGCAACC
ACATATAATTTTACCCAACACCTGTCATTTGTTGATATGGGGCTCGGGTTCAGCTCCACCACTGCAACCG
CCAATCTGAAAAGGATCAAATCAGACATGGGGTCAAAAATCCAGAACCTTTTTAGCGCGTTCCCGATTCA
CGCTTTCACCAATGCAGATATAAACACCTGGATTCGGCACCACGTCGGGATCGAGAAGCCTAACCCATCA
GAAAGCGAAGCCCTGAACATTATCACCTTCGGTGGAATCAATAAGAACCCTCCTTCTATCCTCTTGCACG
GACAACAGGCTATTTGTGAGGTTATTCTGACACCAGTGACTACCAACATCAATTTCTTCAAGTCCCCACA
CAACCCCCGAGGGAGGGAGTCATGCATGATGGGAACTGACCCACACAACGAAGAGGCTGCCAGAAAAGCT
CTCTATGATCATACTCAGACCGACTCTGACACCTTCGCGGCAACAACTAATCCCTGGGCAAGTCTCCCCG
GGTCACTGGGCGACATACTGTACAACACTGCTCACAGGGAGCAGCTCTGCTATAATCCAAAAACCTACTC
ACCAAACGCTCAATTCTTTACCGAGTCAGACATCCTTAAGACCAACAAAATGATGTACAAGGTGATTTCC
GAATATTGCATGAAATCCAATTCATGTCTGAACAGCGATTCTGAGATTCAATATTCCTGCTCCGAAGGCA
CTGATTCCTTTGTAAGCAGGCCATGTCAATTTCTGCAGAATGCATTGCCCCTTCACTGTTCCAGTAACCA
AGCCCTCTTGGAGTCCCGCTCTAAAACAGGTAACACACAAATTAGCGAAACCCACTACTGTAACTATGCC
ATCGGTGAGACGATCCCTTTTCAGCTCATCATCGAGTCCTCCATA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC101407 representing NP_042950
Red=Cloning sites Green=Tags

MENWQATEILPKIEAPLNIFNDIKTYTAEQLFDNLRIYFGDDPSRYNISFEALLGIYCNKIEWINFFTTP
IAVAANVIRFNDVSRMTLGKVLFFIQLPRVATGNDVTASKETTIMVAKHSEKHPINISFDLSAACLEHLE
NTFKNTVIDQILNINALHTVLRSLKNSADSLERGLIHAFMQTLLRKSPPQFIVLTMNENKVHNKQALSRV
QRSNMFQSLKNRLLTSLFFLNRNNNISYIYRILNDMMESVTESILNDTNNYTSKENVPLDGVLLGPIGSI
QKLTSILSQYISTQVVSAPISYGHFIMGKENAVTAIAYRAIMADFTQFTVNAGTEQQDTNNKSEIFDKSR
AYADLKLNTLKLGDKLVAFDHLHKVYKNTDVNDPLEQSLQLTFFFPLGIYIPSETGFSTMETRVKLNDTM
ENNLPTSVFFHNKDQVVQRIDFADILPSVCHPIVHDSTIVERLMKSEPLPTGHRFSQLCQLKITRENPAR
ILQTLYNLYESRQEVPKNTNVLKNELNIEDFYKPDNPTLPTERHPFFDLTYIQKNRATEVLCTPRIMIGN
IPLPLAPVSFHEARTNQILEHAKTNCQKYDFTLKIVTESLTSGSYPELAYVIETLVHGNKHAFMILKQVI
SQCISYWFNMKHILLFCNSFEMIMLISNHMGDELIPGAAFAHYRNLVSLIRLVKRTISISNLNEQLCGEP
LVNFANALFDGRLFCPFVHTMPRNDTNAKITADDTPLTQNTVRVRNYEISDVQRMNLIDSSVVFTDNDRP
SNETTILSKIFYFCVLPALSNNKACGAGVNVKELVLDLFYTEPFISPDDYFQENPITSDVLMSLIREGMG
PGYTVANTSCIAKQLFKSLIYINENTKILEVEVSLDPAQRHGNSVHFQSLQHILYNGLCLISPITTLRRY
YQPIPFHRFFSDPGICGTMNADIQVFLNTFPHCQRNDGGFPLPPPLALEFYNWQRTPFSVYSAFCPNSLL
SIMTLAAMHSKLSPVAIAIQSKNKIHPGFAATLVRTDNFDVECLLYSSRAATSIILDDPTVTAEAKDIAT
TYNFTQHLSFVDMGLGFSSTTATANLKRIKSDMGSKIQNLFSAFPIHAFTNADINTWIRHHVGIEKPNPS
ESEALNIITFGGINKNPPSILLHGQQAICEVILTPVTTNINFFKSPHNPRGRESCMMGTDPHNEEAARKA
LYDHTQTDSDTFAATTNPWASLPGSLGDILYNTAHREQLCYNPKTYSPNAQFFTESDILKTNKMMYKVIS
EYCMKSNSCLNSDSEIQYSCSEGTDSFVSRPCQFLQNALPLHCSSNQALLESRSKTGNTQISETHYCNYA
IGETIPFQLIIESSI

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NC_001664
ORF Size 4035 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NC_001664.2, NP_042950
RefSeq ORF 4035 bp
Locus ID 1487939
MW 152.0 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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