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Osteopontin (SPP1) (NM_001040060) Human Over-expression Lysate
CAT＃: LC400422
SPP1 HEK293T cell transient overexpression lysate (as WB positive control)
Size: 100 ug
View other "Osteopontin" proteins (13)
|
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CNY 900.00
| Cited in 1 publication. |
Product images
CNY 1,999.00
CNY 2,700.00
CNY 300.00
CNY 1,430.00
CNY 600.00
Specifications
| Product Data | |
| Description | SPP1 HEK293T cell transient overexpression lysate (as WB positive control) |
| Species | Human |
| Expression Host | HEK293T |
| Expression cDNA Clone or AA Sequence | TrueORF Clone RC210592 |
| Tag | C-Myc/DDK |
| Detection Antibodies | Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100) |
| Predicted MW | 32.4 kDa |
| Components | 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate |
| Storage | The lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20°C. Lysate samples can be reconstituted with SDS Sample Buffer. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples are stable for 12 months from date of receipt when stored at -20°C. |
| Preparation | HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins. |
| Reference Data | |
| RefSeq | NP_001035149 |
| Locus ID | 6696 |
| Cytogenetics | 4q22.1 |
| Protein Families | WB |
| Protein Pathways | ECM-receptor interaction, Focal adhesion, Toll-like receptor signaling pathway |
Citations (1)
| The use of this Proteins has been cited in the following citations: |
|---|
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Osteopontin enhances HIV replication and is increased in the brain and cerebrospinal fluid of HIV-infected individuals
,null,
Journal of Neurovirology
,PubMed ID 21556958
[SPP1]
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| LC421887 | SPP1 HEK293T cell transient overexpression lysate (as WB positive control) |
CNY 900.00 |
|
| LC424632 | SPP1 HEK293T cell transient overexpression lysate (as WB positive control) |
CNY 900.00 |
|
| LY400422 | Transient overexpression lysate of secreted phosphoprotein 1 (SPP1), transcript variant 3 |
CNY 3,080.00 |
|
| LY421887 | Transient overexpression lysate of secreted phosphoprotein 1 (SPP1), transcript variant 1 |
CNY 3,080.00 |
|
| LY424632 | Transient overexpression lysate of secreted phosphoprotein 1 (SPP1), transcript variant 2 |
CNY 3,080.00 |
|
| PH304803 | SPP1 MS Standard C13 and N15-labeled recombinant protein (NP_001035147) |
CNY 14,250.00 |
|
| PH305118 | SPP1 MS Standard C13 and N15-labeled recombinant protein (NP_000573) |
CNY 14,250.00 |
|
| PH310592 | SPP1 MS Standard C13 and N15-labeled recombinant protein (NP_001035149) |
CNY 19,520.00 |
|
| TP304803 | Purified recombinant protein of Homo sapiens secreted phosphoprotein 1 (SPP1), transcript variant 1, 20 µg |
CNY 2,900.00 |
|
| TP305118 | Purified recombinant protein of Homo sapiens secreted phosphoprotein 1 (SPP1), transcript variant 2, 20 µg |
CNY 2,900.00 |
|
| TP310592 | Purified recombinant protein of human secreted phosphoprotein 1 (SPP1), transcript variant 3, with C-terminal MYC/DDK tag, secretory expressed in HEK293 cells, 20 µg |
CNY 2,900.00 |
|
| TP710213 | Purified protein of Homo sapiens secreted phosphoprotein 1 (SPP1), transcript variant 1, residues 17-314aa, secretory expressed with GP67 signal peptide, with C-terminal DDK tag, expressed in sf9, 20ug |
CNY 4,020.00 |
|
| TP720449 | Recombinant protein of human secreted phosphoprotein 1 (SPP1), transcript variant 2 |
CNY 1,800.00 |
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