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Osteopontin (SPP1) (NM_001040060) Human Recombinant Protein
CAT＃: TP310592
Purified recombinant protein of human secreted phosphoprotein 1 (SPP1), transcript variant 3, with C-terminal MYC/DDK tag, secretory expressed in HEK293 cells, 20 µg
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| Cited in 1 publication. |
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Specifications
| Product Data | |
| Species | Human |
| Expression Host | HEK293T |
| Expression cDNA Clone or AA Sequence |
>RC210592 protein sequence
Red=Cloning site Green=Tags(s) MRIAVICFCLLGITCAIPVKQADSGSSEEKQNAVSSEETNDFKQETLPSKSNESHDHMDDMDDEDDDDHV DSQDSIDSNDSDDVDDTDDSHQSDESHHSDESDELVTDFPTDLPATEVFTPVVPTVDTYDGRGDSVVYGL RSKSKKFRRPDIQYPDATDEDITSHMESEELNGAYKAIPVAQDLNAPSDWDSRGKDSYETSQLDDQSAET HSHKQSRLYKRKANDESNEHSDVIDSQELSKVSREFHSHEFHSHEDMLVVDPKSKEEDKHLKFRISHELD SASSEVN TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Tag | C-Myc/DDK |
| Predicted MW | 30.6 kDa |
| Concentration | >0.05 µg/µL as determined by microplate BCA method |
| Purity | > 80% as determined by SDS-PAGE and Coomassie blue staining |
| Buffer | 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol |
| Preparation | Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. |
| Note | For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process. |
| Storage | Store at -80°C. |
| Stability | Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles. |
| Bioactivity | Cell treatment (PMID: 29959420) |
| Reference Data | |
| RefSeq | NP_001035149 |
| Locus ID | 6696 |
| UniProt ID | P10451 |
| Refseq Size | 1560 |
| Cytogenetics | 4q22.1 |
| Refseq ORF | 861 |
| Synonyms | BNSP; BSPI; ETA-1; OPN |
| Summary | The protein encoded by this gene is involved in the attachment of osteoclasts to the mineralized bone matrix. The encoded protein is secreted and binds hydroxyapatite with high affinity. The osteoclast vitronectin receptor is found in the cell membrane and may be involved in the binding to this protein. This protein is also a cytokine that upregulates expression of interferon-gamma and interleukin-12. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011] |
| Protein Families | Druggable Genome, Secreted Protein |
| Protein Pathways | ECM-receptor interaction, Focal adhesion, Toll-like receptor signaling pathway |
Citations (1)
| The use of this Proteins has been cited in the following citations: |
|---|
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Osteopontin isoforms differentially promote arteriogenesis in response to ischemia via macrophage accumulation and survival
,Lee, GS;Salazar, HF;Joseph, G;Lok, ZSY;Caroti, CM;Weiss, D;Taylor, WR;Lyle, AN;,
Lab. Invest.
,PubMed ID 29959420
[SPP1]
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| LC400422 | SPP1 HEK293T cell transient overexpression lysate (as WB positive control) |
CNY 900.00 |
|
| LC421887 | SPP1 HEK293T cell transient overexpression lysate (as WB positive control) |
CNY 900.00 |
|
| LC424632 | SPP1 HEK293T cell transient overexpression lysate (as WB positive control) |
CNY 900.00 |
|
| LY400422 | Transient overexpression lysate of secreted phosphoprotein 1 (SPP1), transcript variant 3 |
CNY 3,080.00 |
|
| LY421887 | Transient overexpression lysate of secreted phosphoprotein 1 (SPP1), transcript variant 1 |
CNY 3,080.00 |
|
| LY424632 | Transient overexpression lysate of secreted phosphoprotein 1 (SPP1), transcript variant 2 |
CNY 3,080.00 |
|
| PH304803 | SPP1 MS Standard C13 and N15-labeled recombinant protein (NP_001035147) |
CNY 14,250.00 |
|
| PH305118 | SPP1 MS Standard C13 and N15-labeled recombinant protein (NP_000573) |
CNY 14,250.00 |
|
| PH310592 | SPP1 MS Standard C13 and N15-labeled recombinant protein (NP_001035149) |
CNY 19,520.00 |
|
| TP304803 | Purified recombinant protein of Homo sapiens secreted phosphoprotein 1 (SPP1), transcript variant 1, 20 µg |
CNY 2,900.00 |
|
| TP305118 | Purified recombinant protein of Homo sapiens secreted phosphoprotein 1 (SPP1), transcript variant 2, 20 µg |
CNY 2,900.00 |
|
| TP710213 | Purified protein of Homo sapiens secreted phosphoprotein 1 (SPP1), transcript variant 1, residues 17-314aa, secretory expressed with GP67 signal peptide, with C-terminal DDK tag, expressed in sf9, 20ug |
CNY 4,020.00 |
|
| TP720449 | Recombinant protein of human secreted phosphoprotein 1 (SPP1), transcript variant 2 |
CNY 1,800.00 |
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