PP2A-alpha (PPP2CA) (NM_002715) Human Over-expression Lysate
CAT#: LC419151
PPP2CA HEK293T cell transient overexpression lysate (as WB positive control)
Size: 100 ug
View other "PP2A-alpha" proteins (4)
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CNY 900.00
CNY 300.00
CNY 1,430.00
CNY 600.00
Specifications
Product Data | |
Description | PPP2CA HEK293T cell transient overexpression lysate (as WB positive control) |
Species | Human |
Expression Host | HEK293T |
Expression cDNA Clone or AA Sequence | TrueORF Clone RC201334 |
Tag | C-Myc/DDK |
Detection Antibodies | Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100) |
Predicted MW | 35.6 kDa |
Components | 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate |
Storage | The lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20°C. Lysate samples can be reconstituted with SDS Sample Buffer. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples are stable for 12 months from date of receipt when stored at -20°C. |
Preparation | HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins. |
Reference Data | |
RefSeq | NP_002706 |
Locus ID | 5515 |
Cytogenetics | 5q31.1 |
Protein Families | Druggable Genome, Phosphatase, Transcription Factors |
Protein Pathways | Long-term depression, Oocyte meiosis, TGF-beta signaling pathway, Tight junction, Wnt signaling pathway |
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Other Versions
SKU | Description | Size | Price |
---|---|---|---|
LY419151 | Transient overexpression lysate of protein phosphatase 2 (formerly 2A), catalytic subunit, alpha isoform (PPP2CA) |
CNY 3,080.00 |
|
PH301334 | PPP2CA MS Standard C13 and N15-labeled recombinant protein (NP_002706) |
CNY 19,520.00 |
|
TP301334 | Recombinant protein of human protein phosphatase 2 (formerly 2A), catalytic subunit, alpha isoform (PPP2CA), 20 µg |
CNY 2,900.00 |
|
TP750222 | Purified recombinant protein of Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform , full length(266Ser, 269Ser), with N-terminal His tag, expressed in E.coli, 50ug |
CNY 1,950.00 |
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