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Isocitrate dehydrogenase (IDH1) (NM_005896) Human Over-expression Lysate
CAT＃: LY401782
Transient overexpression lysate of isocitrate dehydrogenase 1 (NADP+), soluble (IDH1)
Size: 20 ug
View other "Isocitrate dehydrogenase" proteins (19)
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CNY 3,080.00
| Cited in 1 publication. |
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Specifications
| Product Data | |
| Description | Transient overexpression lysate of isocitrate dehydrogenase 1 (NADP+), soluble (IDH1) |
| Species | Human |
| Expression Host | HEK293T |
| Expression cDNA Clone or AA Sequence | TrueORF Clone RC210582 |
| Tag | C-Myc/DDK |
| Detection Antibodies | Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100) |
| Predicted MW | 46.5 kDa |
| Components | 1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer 1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer 1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT) |
| Storage | The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 2xSDS Sample Buffer provided. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. |
| Preparation | HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). |
| Reference Data | |
| RefSeq | NP_005887 |
| Locus ID | 3417 |
| Cytogenetics | 2q34 |
| Protein Families | WB |
| Protein Pathways | Citrate cycle (TCA cycle), Glutathione metabolism, Metabolic pathways |
Citations (1)
| The use of this Proteins has been cited in the following citations: |
|---|
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Antibody Validation for Western blot: By the User, for the User
,Pillai-Kastoori, L;Heaton, S;Shiflett, SD;Roberts, AC;Solache, A;Schutz-Geschwender, AR;,
J. Biol. Chem.
,PubMed ID 31819006
[Isocitrate dehydrogenase]
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| LC401782 | IDH1 HEK293T cell transient overexpression lysate (as WB positive control) |
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| TP700039 | Recombinant protein of human isocitrate dehydrogenase 1 (NADP+), soluble (IDH1 mutant R132C),with C-terminal Myc-DDK tag, expressed in HEK293 cells |
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| TP700042 | Recombinant protein of human isocitrate dehydrogenase 1 (NADP+), soluble (IDH1 mutant R132G),with C-terminal Myc-DDK tag, expressed in HEK293 cells |
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| TP710042 | Recombinant protein of human isocitrate dehydrogenase 1 (NADP+), soluble (IDH1), with C-terminal polyhistidine tag, expressed in sf9 cells. |
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| TP710043 | Recombinant protein of mutant(R132H) of human isocitrate dehydrogenase 1 (NADP+), soluble (IDH1),with C-terminal DDK tag,expressed in sf9 cells. |
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| TP710044 | Recombinant protein of mutant(R132C) of human isocitrate dehydrogenase 1 (NADP+), soluble (IDH1),with C-terminal DDK tag,expressed in sf9 cells |
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| TP710045 | Recombinant protein of mutant(R132L) of human isocitrate dehydrogenase 1 (NADP+), soluble (IDH1),with C-terminal DDK tag,expressed in sf9 cells. |
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| TP710046 | Recombinant protein of mutant(R132S) of human isocitrate dehydrogenase 1 (NADP+), soluble (IDH1),with C-terminal DDK tag,expressed in sf9 cells. |
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| TP710047 | Recombinant protein of mutant(R132G) of human isocitrate dehydrogenase 1 (NADP+), soluble (IDH1),with C-terminal DDK tag,expressed in sf9 cells. |
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| TP710048 | Recombinant protein of human isocitrate dehydrogenase 1 (NADP+), soluble (IDH1) and mutant(R132H) heterodimer, with C-terminal polyhistidine and DDK tags, expressed in sf9 cells. |
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| TP710049 | Recombinant protein of human isocitrate dehydrogenase 1 (NADP+), soluble (IDH1) and mutant(R132C) heterodimer, with C-terminal polyhistidine and DDK tags, expressed in sf9 cells |
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| TP710050 | Recombinant protein of human isocitrate dehydrogenase 1 (NADP+), soluble (IDH1) and mutant(R132L) heterodimer, with C-terminal polyhistidine and DDK tags, expressed in sf9 cells |
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| TP710051 | Recombinant protein of human isocitrate dehydrogenase 1 (NADP+), soluble (IDH1) and mutant(R132S) heterodimer, with C-terminal polyhistidine and DDK tags, expressed in sf9 cells |
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| TP710052 | Recombinant protein of human isocitrate dehydrogenase 1 (NADP+), soluble (IDH1) and mutant(R132G) heterodimer, with C-terminal polyhistidine and DDK tags, expressed in sf9 cells |
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