DNMT1 (NM_001130823) Human Over-expression Lysate
CAT#: LC427274
DNMT1 HEK293T cell transient overexpression lysate (as WB positive control)
Size: 100 ug
View other "DNMT1" proteins (3)
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CNY 1,470.00
Cited in 2 publications. |
CNY 300.00
CNY 1,430.00
CNY 600.00
Specifications
Product Data | |
Description | DNMT1 HEK293T cell transient overexpression lysate (as WB positive control) |
Species | Human |
Expression Host | HEK293T |
Expression cDNA Clone or AA Sequence | TrueORF Clone RC226414 |
Tag | C-Myc/DDK |
Detection Antibodies | Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100) |
Predicted MW | 184.6 kDa |
Components | 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate |
Storage | The lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20°C. Lysate samples can be reconstituted with SDS Sample Buffer. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples are stable for 12 months from date of receipt when stored at -20°C. |
Preparation | HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins. |
Reference Data | |
RefSeq | NP_001124295 |
Locus ID | 1786 |
Cytogenetics | 19p13.2 |
Protein Families | Druggable Genome, Transcription Factors |
Protein Pathways | Cysteine and methionine metabolism, Metabolic pathways |
Citations (2)
The use of this Proteins has been cited in the following citations: |
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Targeting EP4 downstream c‐Jun through ERK1/2‐mediated reduction of DNMT1 reveals novel mechanism of solamargine‐inhibited growth of lung cancer cells
,null,
Journal of Cellular and Molecular Medicine
,PubMed ID 27620163
[DNMT1]
|
Inhibition of EZH2 via activation of SAPK/JNK and reduction of p65 and DNMT1 as a novel mechanism in inhibition of human lung cancer cells by polyphyllin I
,null,
Journal of Experimental & Clinical Cancer Research : CR
,PubMed ID 27421653
[DNMT1]
|
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Other Versions
SKU | Description | Size | Price |
---|---|---|---|
LY427274 | Transient overexpression lysate of DNA (cytosine-5-)-methyltransferase 1 (DNMT1), transcript variant 1 |
CNY 4,840.00 |
|
PH326414 | DNMT1 MS Standard C13 and N15-labeled recombinant protein (NP_001124295) |
CNY 19,520.00 |
|
TP326414 | Recombinant protein of human DNA (cytosine-5-)-methyltransferase 1 (DNMT1), transcript variant 1, 20 µg |
CNY 2,900.00 |