UL6 (NC_001806) Virus Tagged ORF Clone

CAT#: VC100793

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for capsid portal protein [Human herpesvirus 1], codon optimized for human cell expression, NP_044607



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CNY 10,170.00


货期*
6周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 100 ul

CNY 600.00


Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
    • 100 ul

CNY 1,050.00

Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC100793 represents NCBI reference of NP_044607 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACAGCACCCCGAAGCCGAGCACCTACAACCCGAGCACGCGGGGACACGGAAGCACTTTGCAGTCCCG
AAGACGGCTGGGTTAAGGTCCACCCATCACCCGGTACCATGTTGTTTCGCGAAATTCTGCACGGTCAGCT
GGGCTACACTGAGGGACAAGGGGTTTACAACGTCGTCCGGTCATCCGAAGCCACAACTCGGCAGCTGCAA
GCTGCCATTTTTCACGCTCTGCTGAACGCGACAACCTATCGGGATTTGGAAGCAGACTGGCTCGGGCATG
TAGCCGCCCGCGGCCTGCAGCCCCAGCGGCTGGTCAGGCGCTATAGGAATGCGAGGGAGGCAGATATTGC
TGGTGTGGCTGAACGGGTCTTCGATACTTGGAGAAACACACTGAGAACTACTCTGCTGGACTTCGCGCAC
GGGCTGGTCGCTTGCTTCGCCCCAGGAGGCCCGTCTGGGCCTAGCTCATTCCCCAAATATATTGATTGGC
TGACGTGCCTGGGCCTGGTCCCCATCCTTAGGAAACGACAGGAGGGAGGCGTGACTCAGGGGTTGAGAGC
ATTCTTGAAACAGCATCCTCTTACCCGCCAGCTTGCCACCGTTGCCGAGGCGGCAGAGCGCGCGGGGCCT
GGATTCTTCGAATTGGCCCTGGCTTTTGACTCAACTCGCGTGGCTGATTATGATAGGGTGTATATCTATT
ACAATCATCGAAGAGGAGACTGGCTCGTCCGAGACCCGATTTCTGGCCAGAGGGGGGAATGCCTTGTGCT
CTGGCCACCCCTGTGGACCGGGGATAGATTGGTGTTCGACTCCCCAGTCCAGCGGCTGTTTCCTGAAATC
GTGGCCTGCCACTCCCTGCGAGAACATGCGCATGTGTGTCGGCTGAGAAACACTGCCTCTGTTAAAGTAC
TTCTGGGGCGGAAATCCGATAGCGAGCGCGGTGTGGCCGGCGCAGCCCGGGTGGTGAACAAGGTGCTGGG
AGAAGACGACGAGACCAAAGCAGGAAGTGCGGCGTCCCGCTTGGTGAGGTTGATAATCAACATGAAAGGC
ATGCGACACGTGGGAGATATAAACGACACTGTGCGGTCTTACCTCGACGAAGCTGGGGGGCATTTGATAG
ACGCCCCAGCTGTGGATGGAACCCTCCCCGGTTTTGGCAAGGGCGGAAATAGTCGGGGGAGTGCCGGACA
GGACCAAGGGGGGAGAGCACCCCAGCTTCGCCAAGCATTTCGCACTGCAGTCGTTAATAACATCAACGGA
GTCCTTGAGGGATACATCAATAATTTGTTTGGAACAATTGAGCGCCTGCGCGAGACGAACGCCGGGCTGG
CCACACAACTTCAGGAACGGGACAGAGAGCTTAGGAGAGCCACAGCTGGGGCCTTGGAAAGACAGCAGAG
AGCAGCAGATTTGGCAGCCGAAAGCGTTACAGGAGGCTGCGGTAGTCGACCTGCTGGCGCAGACCTGCTG
CGGGCTGATTACGACATCATAGATGTCAGTAAATCCATGGACGACGACACATACGTAGCGAACAGTTTCC
AGCATCCATATATTCCCTCCTATGCTCAGGACCTGGAGCGACTGTCACGCTTGTGGGAACACGAGCTTGT
CCGATGTTTCAAGATCCTGTGTCACCGGAACAATCAGGGGCAGGAGACCAGTATTTCCTATTCCAGCGGT
GCCATTGCAGCCTTTGTCGCTCCATACTTTGAATCCGTGTTGAGGGCTCCTCGGGTGGGCGCCCCTATCA
CAGGGTCCGATGTGATACTTGGAGAGGAAGAACTCTGGGATGCCGTGTTCAAAAAGACCCGGTTGCAGAC
GTATCTGACCGACATTGCTGCACTCTTCGTCGCGGATGTTCAGCATGCGGCGCTTCCCCCCCCTCCTTCC
CCAGTTGGAGCTGACTTTCGGCCCGGCGCATCTCCTCGCGGTAGAAGTCGGTCACGCAGCCCAGGCCGAA
CCGCACGAGGAGCACCAGACCAAGGAGGAGGAATCGGACACAGGGACGGGAGGCGCGACGGGAGACGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC100793 representing NP_044607
Red=Cloning sites Green=Tags

MTAPRSRAPTTRARGDTEALCSPEDGWVKVHPSPGTMLFREILHGQLGYTEGQGVYNVVRSSEATTRQLQ
AAIFHALLNATTYRDLEADWLGHVAARGLQPQRLVRRYRNAREADIAGVAERVFDTWRNTLRTTLLDFAH
GLVACFAPGGPSGPSSFPKYIDWLTCLGLVPILRKRQEGGVTQGLRAFLKQHPLTRQLATVAEAAERAGP
GFFELALAFDSTRVADYDRVYIYYNHRRGDWLVRDPISGQRGECLVLWPPLWTGDRLVFDSPVQRLFPEI
VACHSLREHAHVCRLRNTASVKVLLGRKSDSERGVAGAARVVNKVLGEDDETKAGSAASRLVRLIINMKG
MRHVGDINDTVRSYLDEAGGHLIDAPAVDGTLPGFGKGGNSRGSAGQDQGGRAPQLRQAFRTAVVNNING
VLEGYINNLFGTIERLRETNAGLATQLQERDRELRRATAGALERQQRAADLAAESVTGGCGSRPAGADLL
RADYDIIDVSKSMDDDTYVANSFQHPYIPSYAQDLERLSRLWEHELVRCFKILCHRNNQGQETSISYSSG
AIAAFVAPYFESVLRAPRVGAPITGSDVILGEEELWDAVFKKTRLQTYLTDIAALFVADVQHAALPPPPS
PVGADFRPGASPRGRSRSRSPGRTARGAPDQGGGIGHRDGRRDGRR

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NC_001806
ORF Size 2028 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NC_001806.1, NP_044607
RefSeq ORF 2028 bp
Locus ID 2703429
UniProt ID P04413
MW 74.1 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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