US8 (NC_001806) Virus Tagged ORF Clone

CAT#: VC100856

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for envelope glycoprotein E [Human herpesvirus 1], codon optimized for human cell expression, NP_044670



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CNY 6,650.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 100 ul

CNY 600.00


Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
    • 100 ul

CNY 1,050.00

Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC100856 represents NCBI reference of NP_044670 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGATCGAGGTGCCGTTGTGGGATTTTTGCTGGGAGTCTGCGTTGTGTCATGCCTTGCCGGGACCCCAA
AAACTAGCTGGCGGAGGGTGTCTGTAGGAGAGGATGTCTCACTCCTTCCAGCTCCAGGTCCTACCGGGCG
CGGACCAACCCAGAAGCTTCTGTGGGCTGTTGAACCCCTTGACGGCTGCGGGCCTCTCCACCCCTCATGG
GTGTCCCTGATGCCCCCTAAGCAGGTCCCCGAAACTGTCGTGGACGCGGCATGCATGCGGGCTCCTGTTC
CCCTTGCGATGGCATATGCGCCACCTGCACCCTCAGCAACTGGCGGTTTGCGCACAGATTTTGTGTGGCA
GGAGCGAGCTGCCGTGGTGAATCGGAGCCTGGTGATCCACGGGGTGAGAGAGACCGATAGCGGGCTGTAT
ACGCTGAGCGTTGGTGACATCAAGGACCCCGCCAGACAGGTGGCCTCTGTGGTGCTTGTCGTCCAGCCTG
CTCCAGTGCCTACCCCCCCTCCTACACCAGCTGATTATGACGAAGACGATAACGACGAAGGGGAGGATGA
GAGCCTGGCAGGGACCCCCGCTAGCGGAACACCTCGACTGCCCCCTCCTCCTGCTCCTCCACGCTCCTGG
CCATCAGCACCTGAGGTGTCCCATGTGAGGGGGGTTACTGTAAGAATGGAAACACCCGAGGCAATTCTGT
TTAGCCCAGGCGAGACATTCAGTACTAATGTGTCTATCCACGCCATTGCCCACGACGACCAGACCTACTC
AATGGATGTGGTTTGGCTCCGCTTTGACGTACCCACCTCTTGCGCCGAGATGAGAATCTACGAAAGTTGT
CTGTACCACCCCCAGCTCCCTGAGTGTCTCAGTCCTGCCGATGCCCCTTGCGCTGCTAGCACCTGGACCA
GTCGGCTGGCAGTTCGGAGTTACGCCGGCTGTTCCCGAACTAACCCTCCTCCCAGGTGTAGCGCAGAAGC
TCACATGGAGCCCGTTCCTGGACTTGCCTGGCAGGCTGCCTCTGTCAACTTGGAATTTAGAGATGCATCT
CCACAGCATTCTGGTTTGTACTTGTGCGTGGTATACGTGAATGATCACATCCACGCTTGGGGCCATATCA
CTATCAGCACCGCTGCTCAGTACAGGAATGCAGTTGTCGAGCAACCTCTGCCCCAGCGCGGAGCTGATCT
TGCCGAGCCCACTCACCCTCATGTGGGCGCACCCCCCCACGCTCCTCCTACACATGGCGCTCTGAGATTG
GGCGCTGTTATGGGTGCCGCCCTGTTGCTCTCCGCTCTCGGCTTGAGTGTCTGGGCCTGCATGACGTGTT
GGCGGAGAAGAGCCTGGAGAGCTGTCAAATCCCGGGCCTCCGGCAAAGGCCCTACTTACATCAGGGTGGC
GGATAGCGAGCTGTACGCCGACTGGTCCTCTGACTCCGAAGGTGAACGAGACCAGGTGCCCTGGCTGGCT
CCACCCGAGAGACCAGACAGTCCCAGCACCAACGGGTCTGGATTCGAGATCTTGAGCCCCACCGCACCTA
GTGTATATCCGCGAAGCGATGGTCACCAGTCCAGGCGACAACTGACCACGTTCGGCTCCGGCCGACCCGA
CAGGCGCTACAGCCAAGCCTCCGATTCAAGCGTGTTCTGG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC100856 representing NP_044670
Red=Cloning sites Green=Tags

MDRGAVVGFLLGVCVVSCLAGTPKTSWRRVSVGEDVSLLPAPGPTGRGPTQKLLWAVEPLDGCGPLHPSW
VSLMPPKQVPETVVDAACMRAPVPLAMAYAPPAPSATGGLRTDFVWQERAAVVNRSLVIHGVRETDSGLY
TLSVGDIKDPARQVASVVLVVQPAPVPTPPPTPADYDEDDNDEGEDESLAGTPASGTPRLPPPPAPPRSW
PSAPEVSHVRGVTVRMETPEAILFSPGETFSTNVSIHAIAHDDQTYSMDVVWLRFDVPTSCAEMRIYESC
LYHPQLPECLSPADAPCAASTWTSRLAVRSYAGCSRTNPPPRCSAEAHMEPVPGLAWQAASVNLEFRDAS
PQHSGLYLCVVYVNDHIHAWGHITISTAAQYRNAVVEQPLPQRGADLAEPTHPHVGAPPHAPPTHGALRL
GAVMGAALLLSALGLSVWACMTCWRRRAWRAVKSRASGKGPTYIRVADSELYADWSSDSEGERDQVPWLA
PPERPDSPSTNGSGFEILSPTAPSVYPRSDGHQSRRQLTTFGSGRPDRRYSQASDSSVFW

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NC_001806
ORF Size 1650 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NC_001806.1, NP_044670
RefSeq ORF 1650 bp
Locus ID 2703448
UniProt ID P04413
MW 59.1 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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