UL46 (NC_001806) Virus Tagged ORF Clone

CAT#: VC100834

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for tegument protein VP11/12 [Human herpesvirus 1], codon optimized for human cell expression, NP_044648



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CNY 10,830.00


货期*
6周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 100 ul

CNY 600.00


Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
    • 100 ul

CNY 1,050.00

Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC100834 represents NCBI reference of NP_044648 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCAAAGGAGAACCAGAGGTGCCTCCAGCTTGAGGCTGGCACGATGTCTGACGCCGGCTAACCTGATCC
GAGGCGACAATGCAGGGGTTCCTGAGCGCCGCATTTTCGGCGGATGTCTCCTGCCAACACCAGAGGGATT
GTTGAGCGCTGCAGTTGGAGCCCTTCGGCAGCGCTCCGATGACGCACAACCCGCCTTTCTTACATGTACT
GACCGCTCAGTTAGGCTGGCCGCCCGACAACACAACACTGTGCCTGAAAGCCTCATCGTGGACGGGTTGG
CCAGTGATCCTCATTATGAGTATATACGCCATTATGCCTCAGCTGCCACTCAGGCCCTGGGTGAAGTGGA
GTTGCCAGGCGGACAGTTGAGCAGAGCAATTCTCACCCAGTATTGGAAGTACCTGCAGACTGTGGTACCC
AGTGGGCTGGATGTCCCGGAAGATCCTGTAGGAGATTGTGATCCCTCCCTTCATGTTCTGCTTCGCCCCA
CCTTGGCTCCCAAGCTGCTGGCCAGAACTCCCTTTAAAAGCGGCGCGGTCGCTGCCAAGTACGCCGCCAC
AGTGGCAGGGCTGCGCGACGCATTGCACAGAATTCAGCAGTACATGTTTTTTATGAGACCCGCCGATCCA
TCCCGGCCGAGCACAGACACGGCACTCAGATTGAACGAGCTGCTCGCATACGTGTCTGTGCTGTACCGGT
GGGCCAGCTGGATGCTCTGGACTACGGATAAACATGTGTGTCACAGACTGAGCCCTAGCAATAGGAGATT
TCTCCCTCTTGGGGGGTCCCCAGAAGCTCCAGCAGAGACCTTCGCCCGACATCTGGATAGAGGGCCAAGT
GGGACTACAGGTTCTATGCAGTGCATGGCCTTGAGGGCTGCCGTCAGTGACGTGCTGGGACACCTGACAC
GCCTCGCCAATCTCTGGCAGACTGGGAAAAGAAGCGGTGGGACCTATGGCACGGTGGATACAGTCGTCTC
TACCGTGGAAGTGCTGTCCATCGTCCACCACCACGCTCAGTATATAATTAACGCCACGCTCACTGGCTAC
GGAGTGTGGGCGACCGACTCCCTGAATAATGAGTACTTGAGGGCGGCGGTTGATAGCCAAGAGAGGTTCT
GTCGCACCACTGCCCCGTTGTTTCCTACCATGACCGCCCCCTCTTGGGCACGGATGGAACTGTCAATCAA
AGCCTGGTTCGGGGCCGCCCTGGCTGCTGACCTGCTTCGGAACGGGGCTCCGTCACTTCATTATGAATCA
ATACTGAGGCTGGTAGCCTCCAGAAGAACCACCTGGTCAGCAGGACCCCCCCCAGACGATATGGCATCTG
GACCAGGAGGACACAGGGCCGGGGGTGGCACGTGTCGCGAGAAGATACAGCGGGCGAGGAGAGATAATGA
GCCACCTCCACTGCCAAGACCACGATTGCATTCCACACCCGCATCCACGCGCCGCTTTAGGAGGCGCCGC
GCAGACGGGGCTGGGCCTCCTCTCCCTGATGCTAATGATCCCGTGGCAGAACCGCCTGCTGCTGCCACTC
AGCCTGCAACATACTATACCCACATGGGAGAAGTGCCACCCAGACTCCCTGCACGGAATGTCGCGGGGCC
AGATCGGAGACCCCCGGCTGCCACATGCCCCTTGCTGGTAAGAAGGGCTAGTCTGGGCTCTCTGGACCGG
CCTCGGGTGTGGGGACCTGCACCTGAAGGTGAGCCGGATCAGATGGAGGCCACTTACCTGACCGCCGACG
ACGACGACGATGACGCTAGACGCAAAGCCACTCATGCCGCGTCCGCCCGAGAACGGCACGCTCCCTATGA
GGATGATGAAAGTATTTATGAGACTGTCAGCGAGGACGGCGGGCGGGTTTATGAAGAGATTCCATGGATG
CGCGTATATGAGAATGTCTGCGTGAATACCGCAAATGCTGCACCTGCTTCCCCTTATATCGAGGCAGAGA
ACCCACTTTACGATTGGGGCGGGAGTGCACTGTTTTCACCCCCAGGTCGCACGGGTCCGCCACCACCTCC
ATTGTCTCCAAGTCCCGTGCTGGCTCGACACCGAGCTAACGCCCTGACCAATGACGGTCCCACTAATGTA
GCAGCCCTCTCTGCTTTGCTGACAAAGCTCAAGAGAGAAGGAAGGAGAAGCAGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC100834 representing NP_044648
Red=Cloning sites Green=Tags

MQRRTRGASSLRLARCLTPANLIRGDNAGVPERRIFGGCLLPTPEGLLSAAVGALRQRSDDAQPAFLTCT
DRSVRLAARQHNTVPESLIVDGLASDPHYEYIRHYASAATQALGEVELPGGQLSRAILTQYWKYLQTVVP
SGLDVPEDPVGDCDPSLHVLLRPTLAPKLLARTPFKSGAVAAKYAATVAGLRDALHRIQQYMFFMRPADP
SRPSTDTALRLNELLAYVSVLYRWASWMLWTTDKHVCHRLSPSNRRFLPLGGSPEAPAETFARHLDRGPS
GTTGSMQCMALRAAVSDVLGHLTRLANLWQTGKRSGGTYGTVDTVVSTVEVLSIVHHHAQYIINATLTGY
GVWATDSLNNEYLRAAVDSQERFCRTTAPLFPTMTAPSWARMELSIKAWFGAALAADLLRNGAPSLHYES
ILRLVASRRTTWSAGPPPDDMASGPGGHRAGGGTCREKIQRARRDNEPPPLPRPRLHSTPASTRRFRRRR
ADGAGPPLPDANDPVAEPPAAATQPATYYTHMGEVPPRLPARNVAGPDRRPPAATCPLLVRRASLGSLDR
PRVWGPAPEGEPDQMEATYLTADDDDDDARRKATHAASARERHAPYEDDESIYETVSEDGGRVYEEIPWM
RVYENVCVNTANAAPASPYIEAENPLYDWGGSALFSPPGRTGPPPPPLSPSPVLARHRANALTNDGPTNV
AALSALLTKLKREGRRSR

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NC_001806
ORF Size 2154 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NC_001806.1, NP_044648
RefSeq ORF 2154 bp
Locus ID 2703413
UniProt ID P04413
MW 78.2 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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