UL22 (NC_001806) Virus Tagged ORF Clone

CAT#: VC100809

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for envelope glycoprotein H [Human herpesvirus 1], codon optimized for human cell expression, NP_044623



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CNY 12,730.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 100 ul

CNY 600.00


Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
    • 100 ul

CNY 1,050.00

Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC100809 represents NCBI reference of NP_044623 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGCAACGGACTGTGGTTCGTCGGCGTTATCATTCTGGGCGTCGCCTGGGGACAGGTCCACGATTGGA
CGGAGCAGACTGATCCTTGGTTCCTCGACGGACTCGGCATGGATAGGATGTACTGGCGGGACACAAACAC
TGGCCGCCTGTGGCTCCCAAATACGCCCGATCCTCAGAAGCCCCCACGGGGATTTCTGGCCCCACCAGAC
GAACTGAATCTCACTACCGCCAGCCTCCCACTGCTGCGATGGTACGAGGAGAGGTTCTGCTTTGTGCTCG
TGACAACAGCTGAGTTTCCTCGAGATCCCGGCCAGCTGCTTTACATCCCAAAGACGTACCTGCTGGGCAG
GCCCCCAAACGCAAGCCTGCCAGCACCCACTACCGTCGAACCAACCGCACAGCCCCCTCCTTCCGTAGCG
CCTCTGAAGGGACTGTTGCATAACCCAGCTGCCTCCGTCCTGCTGCGGTCCCGCGCCTGGGTTACCTTTA
GTGCCGTTCCAGATCCTGAGGCTCTTACATTTCCTCGGGGGGATAACGTGGCCACGGCATCACACCCTTC
TGGCCCCCGAGACACTCCTCCACCTCGGCCTCCAGTAGGGGCTCGGAGACATCCGACTACAGAACTCGAC
ATCACTCATCTGCACAACGCAAGCACAACATGGCTGGCGACTAGAGGCCTCCTTCGCAGTCCTGGCAGAT
ACGTGTACTTCTCCCCCTCCGCTAGCACATGGCCTGTAGGAATTTGGACCACCGGCGAATTGGTTCTTGG
ATGTGATGCTGCTCTTGTCAGAGCTCGGTACGGACGGGAATTCATGGGTCTGGTGATTAGTATGCACGAT
AGCCCTCCTGTGGAGGTCATGGTAGTACCAGCTGGACAGACCCTGGATAGAGTGGGAGATCCAGCAGACG
AGAACCCTCCCGGGGCATTGCCAGGACCTCCCGGTGGACCCCGCTACAGGGTGTTTGTGCTGGGCAGTTT
GACCAGAGCAGACAACGGTAGTGCTCTGGATGCACTGAGGAGGGTGGGTGGCTACCCTGAGGAGGGGACT
AATTACGCCCAGTTTCTTTCAAGGGCCTACGCGGAATTCTTCTCCGGGGATGCGGGAGCAGAACAGGGAC
CAAGGCCTCCCCTGTTTTGGCGGCTCACAGGCCTCCTTGCCACATCCGGATTCGCCTTTGTTAATGCCGC
TCACGCCAATGGCGCAGTGTGTCTCAGTGATCTGCTCGGATTTTTGGCCCACAGCAGGGCACTGGCTGGC
TTGGCTGCCCGCGGTGCTGCGGGCTGCGCCGCCGACAGTGTCTTTTTCAATGTCTCAGTGTTGGACCCCA
CTGCTAGGCTGCAGTTGGAGGCTAGGCTGCAGCATCTGGTAGCAGAAATCCTTGAGCGGGAGCAGTCTCT
CGCCCTGCACGCCCTGGGATATCAACTCGCTTTCGTTCTGGATAGTCCCTCTGCGTACGATGCCGTTGCC
CCTTCTGCGGCACACCTGATCGACGCTCTGTACGCTGAATTTCTCGGAGGTCGGGTGCTCACCACCCCTG
TTGTCCATAGAGCACTGTTTTATGCCTCCGCCGTGCTGCGCCAGCCCTTTCTGGCCGGCGTTCCCTCCGC
CGTGCAGCGGGAACGAGCCAGACGCAGCCTGCTGATAGCCAGTGCTTTGTGCACATCTGACGTGGCCGCG
GCTACAAATGCGGATCTGAGGACGGCGCTGGCCAGGGCAGACCATCAAAAAACACTTTTCTGGCTCCCTG
ACCACTTCAGTCCGTGTGCAGCATCTCTTAGGTTTGATCTGGACGAATCCGTCTTTATCCTGGATGCCTT
GGCTCAGGCAACCCGCTCCGAAACTCCAGTGGAAGTGCTCGCTCAGCAAACACACGGGCTGGCAAGTACA
CTTACAAGGTGGGCTCACTACAACGCTCTGATTAGAGCATTCGTTCCAGAGGCTAGCCACCGCTGCGGCG
GCCAAAGCGCAAATGTGGAACCCAGGATTCTCGTACCCATCACACACAATGCCTCATACGTGGTGACCCA
CAGCCCATTGCCTCGCGGCATCGGTTATAAACTGACCGGCGTTGATGTCCGGAGACCACTCTTCCTCACC
TATCTGACTGCAACATGTGAAGGATCAACAAGAGATATTGAATCCAAGAGGCTGGTGCGAACACAGAACC
AACGGGACCTGGGCCTGGTGGGTGCCGTCTTTATGAGATATACCCCCGCAGGGGAGGTTATGAGTGTGCT
GCTGGTGGATACGGATAATACCCAGCAGCAGATTGCTGCTGGACCGACTGAGGGCGCTCCCAGCGTCTTT
AGCAGCGATGTGCCAAGTACAGCTCTGCTGCTTTTCCCGAATGGAACTGTTATCCATCTGCTGGCCTTCG
ATACCCAGCCGGTTGCTGCAATAGCACCGGGTTTCCTGGCAGCATCAGCCCTGGGCGTAGTTATGATTAC
CGCGGCTCTGGCAGGCATCCTGAAAGTGTTGCGCACCAGTGTGCCCTTCTTTTGGAGGCGGGAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC100809 representing NP_044623
Red=Cloning sites Green=Tags

MGNGLWFVGVIILGVAWGQVHDWTEQTDPWFLDGLGMDRMYWRDTNTGRLWLPNTPDPQKPPRGFLAPPD
ELNLTTASLPLLRWYEERFCFVLVTTAEFPRDPGQLLYIPKTYLLGRPPNASLPAPTTVEPTAQPPPSVA
PLKGLLHNPAASVLLRSRAWVTFSAVPDPEALTFPRGDNVATASHPSGPRDTPPPRPPVGARRHPTTELD
ITHLHNASTTWLATRGLLRSPGRYVYFSPSASTWPVGIWTTGELVLGCDAALVRARYGREFMGLVISMHD
SPPVEVMVVPAGQTLDRVGDPADENPPGALPGPPGGPRYRVFVLGSLTRADNGSALDALRRVGGYPEEGT
NYAQFLSRAYAEFFSGDAGAEQGPRPPLFWRLTGLLATSGFAFVNAAHANGAVCLSDLLGFLAHSRALAG
LAARGAAGCAADSVFFNVSVLDPTARLQLEARLQHLVAEILEREQSLALHALGYQLAFVLDSPSAYDAVA
PSAAHLIDALYAEFLGGRVLTTPVVHRALFYASAVLRQPFLAGVPSAVQRERARRSLLIASALCTSDVAA
ATNADLRTALARADHQKTLFWLPDHFSPCAASLRFDLDESVFILDALAQATRSETPVEVLAQQTHGLAST
LTRWAHYNALIRAFVPEASHRCGGQSANVEPRILVPITHNASYVVTHSPLPRGIGYKLTGVDVRRPLFLT
YLTATCEGSTRDIESKRLVRTQNQRDLGLVGAVFMRYTPAGEVMSVLLVDTDNTQQQIAAGPTEGAPSVF
SSDVPSTALLLFPNGTVIHLLAFDTQPVAAIAPGFLAASALGVVMITAALAGILKVLRTSVPFFWRRE

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NC_001806
ORF Size 2514 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NC_001806.1, NP_044623
RefSeq ORF 2514 bp
Locus ID 2703373
UniProt ID P04413
MW 90.4 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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