UL22 (NC_001806) Virus Tagged ORF Clone
CAT#: VC100809
- TrueORF®
Myc-DDK-tagged ORF clone of viral ORF for envelope glycoprotein H [Human herpesvirus 1], codon optimized for human cell expression, NP_044623
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CNY 12,730.00
Product images
CNY 600.00
CNY 1,050.00
Specifications
Product Data | |
Type | Virus Tagged ORF Clone |
Tag | Myc-DDK |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>The Viral ORF clone VC100809 represents NCBI reference of NP_044623 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s) GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCAACGGACTGTGGTTCGTCGGCGTTATCATTCTGGGCGTCGCCTGGGGACAGGTCCACGATTGGA CGGAGCAGACTGATCCTTGGTTCCTCGACGGACTCGGCATGGATAGGATGTACTGGCGGGACACAAACAC TGGCCGCCTGTGGCTCCCAAATACGCCCGATCCTCAGAAGCCCCCACGGGGATTTCTGGCCCCACCAGAC GAACTGAATCTCACTACCGCCAGCCTCCCACTGCTGCGATGGTACGAGGAGAGGTTCTGCTTTGTGCTCG TGACAACAGCTGAGTTTCCTCGAGATCCCGGCCAGCTGCTTTACATCCCAAAGACGTACCTGCTGGGCAG GCCCCCAAACGCAAGCCTGCCAGCACCCACTACCGTCGAACCAACCGCACAGCCCCCTCCTTCCGTAGCG CCTCTGAAGGGACTGTTGCATAACCCAGCTGCCTCCGTCCTGCTGCGGTCCCGCGCCTGGGTTACCTTTA GTGCCGTTCCAGATCCTGAGGCTCTTACATTTCCTCGGGGGGATAACGTGGCCACGGCATCACACCCTTC TGGCCCCCGAGACACTCCTCCACCTCGGCCTCCAGTAGGGGCTCGGAGACATCCGACTACAGAACTCGAC ATCACTCATCTGCACAACGCAAGCACAACATGGCTGGCGACTAGAGGCCTCCTTCGCAGTCCTGGCAGAT ACGTGTACTTCTCCCCCTCCGCTAGCACATGGCCTGTAGGAATTTGGACCACCGGCGAATTGGTTCTTGG ATGTGATGCTGCTCTTGTCAGAGCTCGGTACGGACGGGAATTCATGGGTCTGGTGATTAGTATGCACGAT AGCCCTCCTGTGGAGGTCATGGTAGTACCAGCTGGACAGACCCTGGATAGAGTGGGAGATCCAGCAGACG AGAACCCTCCCGGGGCATTGCCAGGACCTCCCGGTGGACCCCGCTACAGGGTGTTTGTGCTGGGCAGTTT GACCAGAGCAGACAACGGTAGTGCTCTGGATGCACTGAGGAGGGTGGGTGGCTACCCTGAGGAGGGGACT AATTACGCCCAGTTTCTTTCAAGGGCCTACGCGGAATTCTTCTCCGGGGATGCGGGAGCAGAACAGGGAC CAAGGCCTCCCCTGTTTTGGCGGCTCACAGGCCTCCTTGCCACATCCGGATTCGCCTTTGTTAATGCCGC TCACGCCAATGGCGCAGTGTGTCTCAGTGATCTGCTCGGATTTTTGGCCCACAGCAGGGCACTGGCTGGC TTGGCTGCCCGCGGTGCTGCGGGCTGCGCCGCCGACAGTGTCTTTTTCAATGTCTCAGTGTTGGACCCCA CTGCTAGGCTGCAGTTGGAGGCTAGGCTGCAGCATCTGGTAGCAGAAATCCTTGAGCGGGAGCAGTCTCT CGCCCTGCACGCCCTGGGATATCAACTCGCTTTCGTTCTGGATAGTCCCTCTGCGTACGATGCCGTTGCC CCTTCTGCGGCACACCTGATCGACGCTCTGTACGCTGAATTTCTCGGAGGTCGGGTGCTCACCACCCCTG TTGTCCATAGAGCACTGTTTTATGCCTCCGCCGTGCTGCGCCAGCCCTTTCTGGCCGGCGTTCCCTCCGC CGTGCAGCGGGAACGAGCCAGACGCAGCCTGCTGATAGCCAGTGCTTTGTGCACATCTGACGTGGCCGCG GCTACAAATGCGGATCTGAGGACGGCGCTGGCCAGGGCAGACCATCAAAAAACACTTTTCTGGCTCCCTG ACCACTTCAGTCCGTGTGCAGCATCTCTTAGGTTTGATCTGGACGAATCCGTCTTTATCCTGGATGCCTT GGCTCAGGCAACCCGCTCCGAAACTCCAGTGGAAGTGCTCGCTCAGCAAACACACGGGCTGGCAAGTACA CTTACAAGGTGGGCTCACTACAACGCTCTGATTAGAGCATTCGTTCCAGAGGCTAGCCACCGCTGCGGCG GCCAAAGCGCAAATGTGGAACCCAGGATTCTCGTACCCATCACACACAATGCCTCATACGTGGTGACCCA CAGCCCATTGCCTCGCGGCATCGGTTATAAACTGACCGGCGTTGATGTCCGGAGACCACTCTTCCTCACC TATCTGACTGCAACATGTGAAGGATCAACAAGAGATATTGAATCCAAGAGGCTGGTGCGAACACAGAACC AACGGGACCTGGGCCTGGTGGGTGCCGTCTTTATGAGATATACCCCCGCAGGGGAGGTTATGAGTGTGCT GCTGGTGGATACGGATAATACCCAGCAGCAGATTGCTGCTGGACCGACTGAGGGCGCTCCCAGCGTCTTT AGCAGCGATGTGCCAAGTACAGCTCTGCTGCTTTTCCCGAATGGAACTGTTATCCATCTGCTGGCCTTCG ATACCCAGCCGGTTGCTGCAATAGCACCGGGTTTCCTGGCAGCATCAGCCCTGGGCGTAGTTATGATTAC CGCGGCTCTGGCAGGCATCCTGAAAGTGTTGCGCACCAGTGTGCCCTTCTTTTGGAGGCGGGAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >VC100809 representing NP_044623
Red=Cloning sites Green=Tags MGNGLWFVGVIILGVAWGQVHDWTEQTDPWFLDGLGMDRMYWRDTNTGRLWLPNTPDPQKPPRGFLAPPD ELNLTTASLPLLRWYEERFCFVLVTTAEFPRDPGQLLYIPKTYLLGRPPNASLPAPTTVEPTAQPPPSVA PLKGLLHNPAASVLLRSRAWVTFSAVPDPEALTFPRGDNVATASHPSGPRDTPPPRPPVGARRHPTTELD ITHLHNASTTWLATRGLLRSPGRYVYFSPSASTWPVGIWTTGELVLGCDAALVRARYGREFMGLVISMHD SPPVEVMVVPAGQTLDRVGDPADENPPGALPGPPGGPRYRVFVLGSLTRADNGSALDALRRVGGYPEEGT NYAQFLSRAYAEFFSGDAGAEQGPRPPLFWRLTGLLATSGFAFVNAAHANGAVCLSDLLGFLAHSRALAG LAARGAAGCAADSVFFNVSVLDPTARLQLEARLQHLVAEILEREQSLALHALGYQLAFVLDSPSAYDAVA PSAAHLIDALYAEFLGGRVLTTPVVHRALFYASAVLRQPFLAGVPSAVQRERARRSLLIASALCTSDVAA ATNADLRTALARADHQKTLFWLPDHFSPCAASLRFDLDESVFILDALAQATRSETPVEVLAQQTHGLAST LTRWAHYNALIRAFVPEASHRCGGQSANVEPRILVPITHNASYVVTHSPLPRGIGYKLTGVDVRRPLFLT YLTATCEGSTRDIESKRLVRTQNQRDLGLVGAVFMRYTPAGEVMSVLLVDTDNTQQQIAAGPTEGAPSVF SSDVPSTALLLFPNGTVIHLLAFDTQPVAAIAPGFLAASALGVVMITAALAGILKVLRTSVPFFWRRE TRTRRLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
ACCN | NC_001806 |
ORF Size | 2514 bp |
OTI Disclaimer | The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NC_001806.1, NP_044623 |
RefSeq ORF | 2514 bp |
Locus ID | 2703373 |
UniProt ID | P04413 |
MW | 90.4 kDa |
Documents
Product Manuals |
FAQs |
SDS |
Resources
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VC100816 | Myc-DDK-tagged ORF clone of viral ORF for DNA packaging terminase subunit 2 [Human herpesvirus 1], codon optimized for human cell expression, NP_044630 |
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VC100817 | Myc-DDK-tagged ORF clone of viral ORF for single-stranded DNA-binding protein [Human herpesvirus 1], codon optimized for human cell expression, NP_044631 |
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VC100820 | Myc-DDK-tagged ORF clone of viral ORF for DNA packaging protein UL32 [Human herpesvirus 1], codon optimized for human cell expression, NP_044634 |
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VC100821 | Myc-DDK-tagged ORF clone of viral ORF for DNA packaging protein UL33 [Human herpesvirus 1], codon optimized for human cell expression, NP_044635 |
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VC100822 | Myc-DDK-tagged ORF clone of viral ORF for nuclear egress membrane protein [Human herpesvirus 1], codon optimized for human cell expression, NP_044636 |
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VC100823 | Myc-DDK-tagged ORF clone of viral ORF for small capsid protein [Human herpesvirus 1], codon optimized for human cell expression, NP_044637 |
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VC100828 | Myc-DDK-tagged ORF clone of viral ORF for ribonucleotide reductase subunit 2 [Human herpesvirus 1], codon optimized for human cell expression, NP_044642 |
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VC100829 | Myc-DDK-tagged ORF clone of viral ORF for tegument host shutoff protein [Human herpesvirus 1], codon optimized for human cell expression, NP_044643 |
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VC100830 | Myc-DDK-tagged ORF clone of viral ORF for DNA polymerase processivity subunit [Human herpesvirus 1], codon optimized for human cell expression, NP_044644. Note: ORF is codon optimized |
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VC100832 | Myc-DDK-tagged ORF clone of viral ORF for envelope glycoprotein C [Human herpesvirus 1], codon optimized for human cell expression, NP_044646 |
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VC100833 | Myc-DDK-tagged ORF clone of viral ORF for membrane protein UL45 [Human herpesvirus 1], codon optimized for human cell expression, NP_044647 |
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VC100834 | Myc-DDK-tagged ORF clone of viral ORF for tegument protein VP11/12 [Human herpesvirus 1], codon optimized for human cell expression, NP_044648 |
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