UL9 (NC_001806) Virus Tagged ORF Clone

CAT#: VC100796

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for DNA replication origin-binding helicase [Human herpesvirus 1], codon optimized for human cell expression, NP_044610



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CNY 12,920.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 100 ul

CNY 600.00


Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
    • 100 ul

CNY 1,050.00

Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC100796 represents NCBI reference of NP_044610 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCCTTTGTCGGGGGTGCAGAGTCCGGCGATCCTCTGGGCGCAGGACGCCCTATCGGCGACGACGAAT
GCGAGCAGTATACATCCAGCGTATCACTTGCTAGAATGCTTTACGGAGGTGATTTGGCTGAGTGGGTACC
CAGAGTGCACCCAAAGACCACTATCGAGCGGCAACAGCACGGGCCTGTGACCTTTCCCAACGCATCTGCT
CCAACTGCAAGGTGCGTCACCGTAGTGAGAGCGCCTATGGGTAGTGGCAAGACAACCGCTCTGATTCGGT
GGCTGCGCGAAGCAATTCACTCCCCCGACACATCTGTCCTCGTGGTTTCATGCAGACGGTCCTTTACCCA
AACTTTGGCAACCCGCTTTGCCGAGAGCGGACTGGTTGACTTCGTGACCTATTTCTCCTCAACTAATTAT
ATTATGAATGATCGCCCATTTCACAGGCTCATCGTCCAGGTCGAGTCACTGCACCGGGTCGGCCCAAATC
TCCTCAACAACTATGACGTCTTGGTGCTTGATGAAGTTATGTCCACCCTCGGTCAACTGTACTCACCTAC
GATGCAACAACTGGGCAGGGTGGATGCCCTGATGCTTAGGTTGCTTCGCATCTGTCCACGGATCATAGCC
ATGGACGCGACCGCTAATGCCCAGCTGGTAGACTTCCTCTGTGGTCTGAGGGGGGAGAAAAATGTCCATG
TCGTGGTTGGGGAGTACGCAATGCCGGGGTTTTCAGCTAGGAGGTGCTTGTTTCTTCCCCGGCTGGGCAC
CGAACTGTTGCAGGCAGCCCTTAGGCCACCAGGCCCTCCTAGCGGTCCATCTCCAGACGCTTCTCCCGAA
GCGAGGGGGGCCACCTTCTTTGGTGAGCTTGAGGCCAGGTTGGGGGGCGGAGATAACATTTGCATCTTCT
CTTCAACTGTTAGTTTTGCTGAGATCGTCGCACGCTTCTGCCGGCAGTTTACCGATAGGGTTCTCCTGCT
GCATTCTTTGACGCCTCTGGGAGACGTGACAACCTGGGGACAATATCGAGTAGTTATTTACACGACAGTC
GTGACTGTCGGCCTGTCCTTCGATCCACTGCATTTCGATGGAATGTTTGCCTACGTGAAACCTATGAACT
ATGGTCCAGACATGGTCTCAGTGTACCAGTCACTCGGACGGGTAAGAACTCTGAGGAAAGGCGAGCTCTT
GATCTACATGGACGGCAGTGGAGCAAGGAGTGAGCCAGTGTTTACCCCCATGCTTCTTAACCACGTCGTG
TCTTCTTGTGGACAGTGGCCCGCCCAATTCTCACAGGTTACCAATCTGCTGTGCAGGAGATTCAAGGGAA
GGTGTGACGCCTCCGCATGCGACACGTCTCTGGGACGGGGCAGTAGGATTTACAACAAGTTTAGGTATAA
GCATTATTTCGAGAGATGCACCCTGGCCTGCTTGTCCGATAGCCTGAATATCCTGCATATGCTGTTGACC
CTGAACTGCATACGGGTGAGATTCTGGGGACACGATGATACGCTGACTCCAAAAGACTTTTGTCTGTTCC
TCCGCGGGGTACATTTTGACGCCCTGCGGGCTCAAAGAGATCTGCGAGAACTGAGATGCCGGGATCCAGA
GGCTTCCCTCCCAGCCCAAGCAGCAGAGACAGAAGAGGTGGGCCTGTTTGTCGAAAAATATCTTCGGTCC
GATGTGGCGCCTGCAGAGATAGTAGCCCTGATGAGAAACCTGAATAGTCTGATGGGACGAACACGGTTCA
TCTACCTTGCCCTGCTGGAAGCCTGTTTGAGGGTACCAATGGCAACCCGGAGCAGTGCCATCTTCCGGAG
AATATATGACCACTACGCGACAGGCGTTATCCCAACAATCAACGTGACGGGGGAGCTGGAACTTGTCGCT
CTCCCACCAACACTGAACGTAACTCCTGTCTGGGAACTGCTCTGCCTGTGCTCTACCATGGCCGCCCGCT
TGCACTGGGATTCTGCCGCCGGAGGGTCAGGTAGGACATTCGGGCCTGACGACGTACTGGATCTGCTGAC
TCCTCACTATGACAGGTACATGCAGCTCGTGTTCGAACTGGGACATTGCAATGTGACTGACGGTCTGCTG
CTGTCCGAAGAAGCTGTGAAGCGAGTGGCTGATGCCCTTTCCGGTTGCCCCCCGAGAGGGTCCGTTTCTG
AGACTGACCACGCCGTGGCGCTGTTTAAAATCATTTGGGGCGAACTGTTCGGGGTGCAGATGGCCAAGAG
TACACAAACATTTCCAGGTGCGGGCCGAGTCAAGAACCTTACGAAGCAAACGATTGTCGGATTGCTGGAC
GCTCATCATATTGACCACAGCGCTTGCAGGACTCACCGCCAGCTTTATGCTTTGCTGATGGCCCATAAAA
GAGAGTTCGCAGGGGCAAGGTTTAAGTTGCGCGTGCCAGCCTGGGGAAGATGCCTGCGCACACATTCTTC
AAGCGCCAATCCGAATGCCGATATTATTCTGGAGGCTGCCCTCTCTGAACTGCCTACCGAGGCGTGGCCC
ATGATGCAAGGAGCTGTGAATTTCAGTACCCTC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC100796 representing NP_044610
Red=Cloning sites Green=Tags

MPFVGGAESGDPLGAGRPIGDDECEQYTSSVSLARMLYGGDLAEWVPRVHPKTTIERQQHGPVTFPNASA
PTARCVTVVRAPMGSGKTTALIRWLREAIHSPDTSVLVVSCRRSFTQTLATRFAESGLVDFVTYFSSTNY
IMNDRPFHRLIVQVESLHRVGPNLLNNYDVLVLDEVMSTLGQLYSPTMQQLGRVDALMLRLLRICPRIIA
MDATANAQLVDFLCGLRGEKNVHVVVGEYAMPGFSARRCLFLPRLGTELLQAALRPPGPPSGPSPDASPE
ARGATFFGELEARLGGGDNICIFSSTVSFAEIVARFCRQFTDRVLLLHSLTPLGDVTTWGQYRVVIYTTV
VTVGLSFDPLHFDGMFAYVKPMNYGPDMVSVYQSLGRVRTLRKGELLIYMDGSGARSEPVFTPMLLNHVV
SSCGQWPAQFSQVTNLLCRRFKGRCDASACDTSLGRGSRIYNKFRYKHYFERCTLACLSDSLNILHMLLT
LNCIRVRFWGHDDTLTPKDFCLFLRGVHFDALRAQRDLRELRCRDPEASLPAQAAETEEVGLFVEKYLRS
DVAPAEIVALMRNLNSLMGRTRFIYLALLEACLRVPMATRSSAIFRRIYDHYATGVIPTINVTGELELVA
LPPTLNVTPVWELLCLCSTMAARLHWDSAAGGSGRTFGPDDVLDLLTPHYDRYMQLVFELGHCNVTDGLL
LSEEAVKRVADALSGCPPRGSVSETDHAVALFKIIWGELFGVQMAKSTQTFPGAGRVKNLTKQTIVGLLD
AHHIDHSACRTHRQLYALLMAHKREFAGARFKLRVPAWGRCLRTHSSSANPNADIILEAALSELPTEAWP
MMQGAVNFSTL

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NC_001806
ORF Size 2553 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NC_001806.1, NP_044610
RefSeq ORF 2553 bp
Locus ID 2703434
UniProt ID P04413
MW 94.3 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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