UL30 (NC_001806) Virus Tagged ORF Clone

CAT#: VC100818

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for DNA polymerase catalytic subunit [Human herpesvirus 1], codon optimized for human cell expression, NP_044632. Note: ORF is codon optimized



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CNY 9,456.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 100 ul

CNY 600.00


Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
    • 100 ul

CNY 1,050.00

Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>VC100818 representing NP_044632, codon optimized.
Due to the complexity of NP_044632, the ORF clone is codon optimized for mammalian Expression.
The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical.

Blue=ORF Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGTTTAGCGGAGGTGGGGGACCTCTGTCTCCTGGAGGAAAAAGCGCGGCACGAGCCGCATCTGGCTTT
TTCGCACCTGCGGGTCCACGCGGTGCGTCACGCGGCCCACCACCCTGTCTTCGCCAGAACTTCTATAAC
CCCTACCTGGCACCTGTCGGCACTCAACAGAAGCCTACTGGTCCCACCCAAAGGCACACCTACTACTCT
GAGTGCGACGAGTTCCGATTCATTGCCCCGCGAGTGTTGGATGAGGATGCCCCCCCAGAGAAGCGGGCC
GGGGTGCATGATGGACATCTGAAGAGAGCACCGAAGGTTTACTGTGGAGGGGATGAAAGGGATGTGCTT
CGGGTGGGCTCAGGTGGATTTTGGCCCCGAAGAAGTAGACTCTGGGGTGGGGTGGATCATGCTCCTGCC
GGTTTCAATCCCACTGTCACTGTGTTCCACGTGTATGACATTCTTGAAAACGTCGAACACGCTTACGGT
ATGCGAGCCGCTCAGTTTCACGCGAGATTTATGGACGCCATCACACCAACTGGCACCGTGATCACTCTC
CTGGGCCTTACTCCAGAAGGCCACCGCGTTGCTGTGCATGTGTACGGCACCAGGCAGTACTTCTATATG
AACAAAGAAGAGGTGGATAGACACCTTCAGTGTAGAGCACCTCGCGACCTGTGTGAAAGAATGGCAGCA
GCTCTCCGAGAATCACCAGGCGCTAGCTTTCGGGGTATCAGCGCAGACCATTTTGAGGCCGAAGTGGTA
GAGCGCACCGACGTCTACTACTATGAAACTCGACCCGCCCTGTTCTACCGCGTATATGTGCGCTCTGGA
AGAGTTCTCTCTTACCTCTGCGATAACTTTTGTCCTGCCATTAAGAAGTATGAAGGGGGTGTCGACGCT
ACCACACGCTTCATCTTGGATAACCCGGGGTTCGTTACATTCGGTTGGTATCGACTGAAGCCAGGTCGG
AACAATACCCTCGCACAACCAGCGGCCCCGATGGCCTTTGGGACATCCTCCGACGTGGAGTTCAACTGC
ACTGCTGATAACCTGGCTATAGAAGGCGGCATGAGCGACTTGCCAGCATACAAGTTGATGTGCTTTGAT
ATCGAGTGCAAAGCAGGGGGAGAGGACGAACTGGCCTTCCCTGTCGCGGGGCATCCTGAAGATCTGGTG
ATCCAAATTTCATGCCTGCTGTATGATCTCTCCACCACTGCCCTTGAGCATGTGCTTCTCTTTTCTTTG
GGCAGTTGCGACCTGCCCGAGTCTCACCTGAACGAGCTGGCCGCGCGCGGCCTGCCCACCCCAGTGGTC
CTGGAATTCGACAGTGAGTTTGAGATGCTCCTGGCTTTTATGACCCTCGTGAAGCAGTATGGGCCTGAG
TTTGTAACAGGATATAACATAATTAATTTTGACTGGCCCTTTTTGCTCGCAAAACTGACCGACATCTAC
AAAGTACCGCTCGACGGCTATGGGCGCATGAATGGCCGCGGCGTCTTCCGCGTGTGGGACATTGGCCAA
TCCCATTTCCAGAAAAGATCTAAGATTAAGGTTAACGGAATGGTGAATATCGATATGTACGGCATAATC
ACAGACAAGATCAAGTTGAGCAGTTATAAACTTAACGCAGTGGCTGAAGCCGTTCTGAAGGATAAAAAG
AAGGATCTGAGTTACAGGGATATCCCGGCTTACTATGCCGCTGGCCCTGCTCAGCGCGGCGTCATTGGG
GAGTACTGTATCCAGGACAGTCTTCTGGTTGGACAGCTGTTTTTCAAATTTTTGCCCCATCTGGAACTG
TCCGCCGTGGCACGACTGGCCGGAATCAACATAACCCGAACCATTTATGATGGGCAGCAGATCCGGGTA
TTCACATGTCTGTTGAGACTGGCTGACCAGAAAGGTTTCATCCTTCCCGATACTCAGGGAAGATTCAGA
GGCGCCGGGGGCGAGGCCCCTAAGCGACCTGCGGCAGCAAGAGAGGATGAAGAACGGCCAGAAGAAGAG
GGAGAGGATGAAGACGAAAGGGAAGAGGGCGGCGGTGAAAGAGAACCTGAAGGTGCCAGAGAGACGGCC
GGGCGACATGTTGGGTATCAGGGCGCTAGGGTCCTCGATCCCACATCCGGATTCCATGTTAATCCTGTG
GTGGTCTTCGACTTTGCATCTCTCTACCCATCAATAATCCAGGCGCACAACCTTTGCTTCTCTACACTC
TCCCTGAGGGCAGACGCCGTGGCTCACCTGGAAGCCGGGAAAGACTACCTCGAAATTGAGGTGGGGGGT
CGCCGACTGTTCTTTGTGAAAGCTCACGTGCGCGAAAGCCTCCTTAGTATCTTGCTGAGAGATTGGCTC
GCTATGAGAAAACAAATCCGGAGCAGGATTCCCCAGAGTAGCCCAGAAGAAGCCGTTCTTCTTGACAAA
CAGCAGGCTGCCATTAAGGTTGTGTGTAACTCTGTGTATGGCTTTACTGGGGTCCAACACGGGCTGCTC
CCCTGCTTGCACGTGGCTGCTACTGTGACAACGATTGGACGCGAGATGCTGCTTGCCACACGCGAATAC
GTACATGCTCGGTGGGCAGCGTTCGAGCAGCTCCTGGCCGACTTTCCAGAGGCAGCAGATATGAGAGCA
CCCGGGCCGTACTCTATGAGAATTATCTACGGTGACACAGACAGCATTTTCGTGCTTTGCCGGGGACTC
ACGGCCGCAGGCTTGACCGCTGTTGGGGATAAGATGGCCTCACACATAAGTCGCGCTCTGTTCCTGCCC
CCAATCAAGCTGGAGTGCGAAAAAACCTTTACCAAACTGTTGCTTATAGCCAAGAAGAAGTACATTGGG
GTTATCTATGGCGGGAAGATGCTTATCAAAGGAGTCGACCTCGTGCGAAAGAATAACTGCGCCTTCATC
AACAGAACCTCCAGGGCACTTGTTGATCTCTTGTTCTATGACGACACCGTTAGCGGAGCAGCTGCAGCC
CTCGCTGAGCGGCCTGCGGAGGAATGGTTGGCCAGACCACTGCCCGAAGGACTCCAGGCATTCGGTGCC
GTGCTGGTCGATGCTCACAGGCGAATTACTGATCCAGAGCGCGACATTCAGGACTTCGTACTGACCGCC
GAACTCTCACGGCATCCCAGAGCGTACACCAACAAACGCCTCGCTCATTTGACAGTGTATTACAAGCTT
ATGGCTCGGAGAGCTCAAGTCCCATCCATCAAAGATAGAATTCCTTATGTCATTGTCGCGCAGACTCGA
GAGGTTGAGGAAACCGTGGCAAGACTGGCCGCCCTGAGAGAACTGGATGCCGCTGCACCTGGTGATGAG
CCTGCCCCCCCTGCAGCCCTGCCATCACCAGCTAAAAGGCCCAGAGAGACCCCGAGTCCAGCTGATCCA
CCAGGTGGGGCCAGTAAACCTCGAAAACTCCTGGTAAGCGAGCTGGCAGAGGATCCAGCATATGCTATT
GCCCACGGTGTTGCACTTAACACCGACTATTACTTTAGCCATCTCCTGGGGGCTGCTTGTGTTACATTC
AAAGCACTGTTCGGGAACAACGCCAAGATCACCGAGTCCCTCTTGAAGAGATTTATACCAGAGGTCTGG
CATCCCCCCGATGATGTCGCCGCTCGACTGCGCACAGCAGGGTTCGGCGCCGTCGGAGCAGGAGCAACC
GCGGAGGAAACTCGACGAATGCTTCATAGGGCCTTTGATACGTTGGCC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAG
GTTTAAACGGCCGGC
>VC100818 representing NP_044632
Blue=ORF Red=Cloning site Green=Tag(s)

MFSGGGGPLSPGGKSAARAASGFFAPAGPRGASRGPPPCLRQNFYNPYLAPVGTQQKPTGPTQRHTYYS
ECDEFRFIAPRVLDEDAPPEKRAGVHDGHLKRAPKVYCGGDERDVLRVGSGGFWPRRSRLWGGVDHAPA
GFNPTVTVFHVYDILENVEHAYGMRAAQFHARFMDAITPTGTVITLLGLTPEGHRVAVHVYGTRQYFYM
NKEEVDRHLQCRAPRDLCERMAAALRESPGASFRGISADHFEAEVVERTDVYYYETRPALFYRVYVRSG
RVLSYLCDNFCPAIKKYEGGVDATTRFILDNPGFVTFGWYRLKPGRNNTLAQPAAPMAFGTSSDVEFNC
TADNLAIEGGMSDLPAYKLMCFDIECKAGGEDELAFPVAGHPEDLVIQISCLLYDLSTTALEHVLLFSL
GSCDLPESHLNELAARGLPTPVVLEFDSEFEMLLAFMTLVKQYGPEFVTGYNIINFDWPFLLAKLTDIY
KVPLDGYGRMNGRGVFRVWDIGQSHFQKRSKIKVNGMVNIDMYGIITDKIKLSSYKLNAVAEAVLKDKK
KDLSYRDIPAYYAAGPAQRGVIGEYCIQDSLLVGQLFFKFLPHLELSAVARLAGINITRTIYDGQQIRV
FTCLLRLADQKGFILPDTQGRFRGAGGEAPKRPAAAREDEERPEEEGEDEDEREEGGGEREPEGARETA
GRHVGYQGARVLDPTSGFHVNPVVVFDFASLYPSIIQAHNLCFSTLSLRADAVAHLEAGKDYLEIEVGG
RRLFFVKAHVRESLLSILLRDWLAMRKQIRSRIPQSSPEEAVLLDKQQAAIKVVCNSVYGFTGVQHGLL
PCLHVAATVTTIGREMLLATREYVHARWAAFEQLLADFPEAADMRAPGPYSMRIIYGDTDSIFVLCRGL
TAAGLTAVGDKMASHISRALFLPPIKLECEKTFTKLLLIAKKKYIGVIYGGKMLIKGVDLVRKNNCAFI
NRTSRALVDLLFYDDTVSGAAAALAERPAEEWLARPLPEGLQAFGAVLVDAHRRITDPERDIQDFVLTA
ELSRHPRAYTNKRLAHLTVYYKLMARRAQVPSIKDRIPYVIVAQTREVEETVARLAALRELDAAAPGDE
PAPPAALPSPAKRPRETPSPADPPGGASKPRKLLVSELAEDPAYAIAHGVALNTDYYFSHLLGAACVTF
KALFGNNAKITESLLKRFIPEVWHPPDDVAARLRTAGFGAVGAGATAEETRRMLHRAFDTLA

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NC_001806
ORF Size 3705 bp
OTI Disclaimer This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NP_044632
RefSeq ORF 3705 bp
Locus ID 2703462
UniProt ID P04413
MW 136.4 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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