UL12 (NC_001806) Virus Tagged ORF Clone

CAT#: VC100799

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for deoxyribonuclease [Human herpesvirus 1], codon optimized for human cell expression, NP_044613



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CNY 7,510.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 100 ul

CNY 600.00


Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
    • 100 ul

CNY 1,050.00

Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC100799 represents NCBI reference of NP_044613 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAATCAACCGTCGGTCCTGCCTGTCCTCCTGGCCGAACCGTGACCAAACGCCCATGGGCTCTGGCGG
AGGATACACCACGAGGCCCAGATTCTCCTCCCAAGAGGCCAAGGCCAAACTCCCTGCCTCTTACCACTAC
CTTTAGGCCGCTCCCCCCGCCCCCTCAAACCACATCTGCAGTTGACCCAAGTAGTCATAGCCCAGTGAAC
CCCCCACGGGATCAGCATGCAACAGACACAGCCGACGAAAAACCTAGAGCCGCTTCACCTGCTCTGTCTG
ATGCCAGCGGACCACCCACCCCCGACATCCCTCTCTCCCCTGGGGGCACCCACGCTAGGGATCCAGACGC
CGATCCAGACAGCCCCGATTTGGACAGCATGTGGAGTGCCAGTGTGATCCCGAATGCACTGCCTTCACAC
ATACTCGCCGAAACATTTGAGAGACATCTGAGGGGATTGCTCCGAGGAGTGAGGGCTCCACTGGCTATAG
GCCCCTTGTGGGCGCGGTTGGACTACCTCTGTAGCCTCGCGGTTGTCCTTGAGGAGGCTGGCATGGTGGA
TAGAGGGCTCGGGAGACACCTGTGGCGGTTGACAAGGCGCGGACCCCCAGCCGCAGCTGATGCGGTAGCG
CCAAGGCCTCTCATGGGCTTCTACGAGGCCGCTACACAGAACCAGGCCGATTGCCAGCTGTGGGCTCTTC
TGCGCAGGGGTCTCACCACAGCATCCACCCTGAGATGGGGTCCACAGGGACCTTGTTTCAGCCCGCAGTG
GTTGAAGCACAATGCAAGCCTTCGCCCAGATGTTCAGAGTAGCGCAGTTATGTTCGGACGGGTTAACGAG
CCTACAGCCAGGTCCTTGCTCTTTCGGTATTGCGTGGGGCGGGCCGATGACGGGGGTGAGGCCGGGGCCG
ATACGCGCAGGTTCATATTCCACGAGCCCTCAGACCTGGCTGAAGAGAATGTTCACACATGTGGAGTTCT
GATGGACGGTCATACCGGGATGGTTGGTGCCAGCCTCGACATCCTTGTGTGTCCCCGCGACATCCATGGG
TACCTGGCACCCGTCCCCAAGACCCCACTGGCATTCTACGAAGTGAAATGCCGCGCTAAGTACGCCTTTG
ACCCTATGGACCCAAGTGATCCTACCGCTAGTGCCTATGAGGACCTGATGGCTCACAGGTCTCCTGAAGC
CTTTCGGGCCTTTATACGCTCAATTCCAAAACCTAGCGTAAGATACTTCGCCCCTGGAAGGGTGCCCGGC
CCAGAAGAGGCCCTGGTTACACAGGACCAAGCGTGGTCCGAAGCACATGCTAGCGGCGAGAAGCGAAGAT
GCTCTGCTGCCGATAGAGCGCTGGTGGAGTTGAACTCTGGCGTTGTGTCCGAGGTGCTGCTGTTCGGCGC
TCCGGACCTGGGAAGGCACACTATCAGCCCCGTGAGCTGGTCTAGTGGAGATCTGGTTAGACGAGAGCCT
GTTTTTGCCAACCCCAGACATCCTAACTTTAAACAGATTCTGGTGCAAGGTTACGTGCTGGACAGCCACT
TCCCGGATTGTCCTCCACATCCTCATCTGGTGACCTTTATCGGACGGCACCGCACCAGCGCCGAGGAAGG
CGTCACCTTCAGACTTGAGGATGGCGCCGGGGCTCTCGGCGCCGCAGGCCCGAGCAAGGCATCCATTCTG
CCGAATCAGGCGGTCCCTATCGCTCTGATCATAACCCCCGTGCGGATCGACCCTGAGATCTACAAGGCCA
TACAGAGGTCAAGTCGGCTTGCCTTCGACGACACCCTGGCCGAATTGTGGGCTTCTCGGAGTCCCGGACC
CGGACCCGCCGCCGCCGAAACAACTTCTTCATCTCCCACCACAGGACGGAGCTCCCGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC100799 representing NP_044613
Red=Cloning sites Green=Tags

MESTVGPACPPGRTVTKRPWALAEDTPRGPDSPPKRPRPNSLPLTTTFRPLPPPPQTTSAVDPSSHSPVN
PPRDQHATDTADEKPRAASPALSDASGPPTPDIPLSPGGTHARDPDADPDSPDLDSMWSASVIPNALPSH
ILAETFERHLRGLLRGVRAPLAIGPLWARLDYLCSLAVVLEEAGMVDRGLGRHLWRLTRRGPPAAADAVA
PRPLMGFYEAATQNQADCQLWALLRRGLTTASTLRWGPQGPCFSPQWLKHNASLRPDVQSSAVMFGRVNE
PTARSLLFRYCVGRADDGGEAGADTRRFIFHEPSDLAEENVHTCGVLMDGHTGMVGASLDILVCPRDIHG
YLAPVPKTPLAFYEVKCRAKYAFDPMDPSDPTASAYEDLMAHRSPEAFRAFIRSIPKPSVRYFAPGRVPG
PEEALVTQDQAWSEAHASGEKRRCSAADRALVELNSGVVSEVLLFGAPDLGRHTISPVSWSSGDLVRREP
VFANPRHPNFKQILVQGYVLDSHFPDCPPHPHLVTFIGRHRTSAEEGVTFRLEDGAGALGAAGPSKASIL
PNQAVPIALIITPVRIDPEIYKAIQRSSRLAFDDTLAELWASRSPGPGPAAAETTSSSPTTGRSSR

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NC_001806
ORF Size 1878 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NC_001806.1, NP_044613
RefSeq ORF 1878 bp
Locus ID 2703382
UniProt ID P04413
MW 67.5 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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