UL5 (NC_001798) Virus Tagged ORF Clone

CAT#: VC100869

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for helicase-primase helicase subunit [Human herpesvirus 2], codon optimized for human cell expression, NP_044474



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CNY 13,300.00


货期*
6周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 100 ul

CNY 600.00


Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
    • 100 ul

CNY 1,050.00

Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC100869 represents NCBI reference of NP_044474 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTGCCTCCGGAGGCGAGGGCAGCAGGGACGTAAGGGCTCCCGGACCTCCGCCTCAACAGCCAGGTG
CCAGACCTGCAGTGAGATTCAGAGATGAGGCTTTTTTGAACTTTACCTCCATGCACGGAGTCCAGCCCAT
AATCGCAAGAATCAGGGAGCTGTCCCAGCAGCAGCTGGATGTGACTCAAGTCCCGCGGTTGCAATGGTTC
CGCGACGTTGCCGCCCTGGAGGTGCCAACAGGACTGCCTTTGCGCGAGTTCCCGTTTGCCGCTTATCTGA
TAACAGGAAACGCTGGGAGTGGGAAATCCACCTGCGTGCAAACCCTGAACGAAGTCCTGGACTGCGTGGT
GACCGGAGCGACTCGCATTGCAGCACAGAACATGTACGTCAAACTTAGTGGCGCATTCCTCAGTAGGCCG
ATTAATACCATCTTCCACGAGTTCGGGTTTCGGGGGAACCACGTTCAAGCACAGCTGGGACAACACCCAT
ATACACTGGCCTCCTCCCCCGCTTCCCTCGAAGATTTGCAGAGACGGGACCTGACATACTACTGGGAGGT
GATCCTTGACATCACTAAGAGAGCCCTGGCCGCCCACGGGGGAGAAGACGCTAGAAATGAGTTCCATGCG
CTCACCGCCCTGGAACAGACCCTGGGATTGGGACAGGGAGCCCTTACCCGCCTGGCATCTGTGACTCACG
GAGCTCTGCCGGCTTTCACAAGAAGCAACATTATAGTTATAGATGAGGCCGGTCTGCTGGGGAGGCACCT
CCTGACTACAGTAGTGTACTGTTGGTGGATGATTAACGCTCTGTACCACACACCACAGTACGCCGGTAGA
TTGCGCCCTGTTCTGGTATGTGTCGGGTCTCCGACACAGACAGCCTCCCTTGAGTCTACATTCGAGCATC
AGAAGCTGAGATGTTCAGTGAGACAGTCTGAAAATGTGCTGACATATCTCATTTGTAACAGGACACTGCG
AGAATATACCCGACTTTCTCACTCCTGGGCCATATTCATCAATAATAAAAGATGCGTGGAGCATGAGTTC
GGGAATTTGATGAAAGTTCTGGAGTACGGGTTGCCAATCACAGAAGAGCACATGCAATTCGTAGACCGGT
TTGTGGTGCCTGAATCTTACATAACCAACCCAGCCAACCTGCCTGGCTGGACCAGACTTTTCTCCAGTCA
TAAAGAAGTGTCAGCCTATATGGCTAAACTGCACGCCTATCTCAAGGTGACGAGAGAGGGGGAATTCGTT
GTGTTCACTCTGCCAGTTCTCACATTTGTTTCCGTCAAGGAGTTCGATGAGTACAGGCGCCTGACCCAGC
AGCCAACCCTTACTATGGAAAAGTGGATTACTGCCAACGCTTCTCGGATCACTAATTATAGCCAGTCACA
GGACCAAGATGCAGGACACGTACGCTGTGAAGTTCACTCAAAACAACAGCTGGTAGTGGCGAGAAATGAT
ATCACCTACGTTCTGAACTCCCAGGTGGCCGTAACTGCACGCCTGAGAAAGATGGTGTTCGGCTTTGATG
GAACTTTCAGAACTTTCGAGGCCGTATTGCGGGACGACAGTTTCGTGAAGACCCAGGGGGAAACCTCAGT
GGAATTCGCCTATCGGTTTCTGTCCCGGCTGATGTTCGGAGGTCTGATACACTTTTACAACTTTCTCCAA
CGACCAGGCTTGGACGCCACCCAGAGAACCCTGGCTTATGGGCGGTTGGGTGAACTTACAGCTGAACTGC
TGAGCCTGCGCAGAGACGCCGCCGGGGCCTCCGCAACTCGAGCTGCTGATACAAGCGACCGGAGTCCCGG
AGAGAGAGCTTTCAACTTTAAACATTTGGGACCCAGGGACGGAGGTCCGGACGATTTTCCCGACGACGAC
CTGGACGTCATTTTTGCCGGCCTGGACGAACAGCAGTTGGACGTGTTCTATTGTCATTACGCCCTGGAGG
AACCGGAGACCACCGCAGCTGTCCATGCCCAGTTCGGTCTGCTTAAGAGGGCGTTTCTGGGACGGTATCT
GATCCTGCGGGAGCTGTTCGGAGAAGTGTTTGAATCTGCGCCTTTCAGTACATACGTGGATAACGTAATC
TTCAGGGGCTGCGAGCTCCTGACCGGTAGCCCACGCGGAGGTCTGATGAGCGTGGCGTTGCAGACAGACA
ACTACACTTTGATGGGCTATACATACACTAGGGTCTTCGCATTTGCCGAAGAGCTGAGAAGGCGGCACGC
TACTGCAGGCGTCGCCGAATTTCTCGAAGAGAGCCCTCTGCCATACATAGTGCTGCGAGACCAACACGGC
TTCATGTCCGTGGTTAACACAAATATCAGCGAGTTCGTAGAGTCAATCGACAGTACAGAACTGGCTATGG
CAATTAATGCTGATTACGGCATCTCTTCTAAACTGGCTATGACCATTACAAGATCTCAAGGATTGTCTCT
GGACAAGGTCGCTATTTGTTTTACACCCGGGAATCTTCGACTGAACAGCGCATACGTGGCTATGAGTAGG
ACAACCAGCTCCGAGTTTCTGCACATGAATCTGAACCCCCTGCGAGAGAGACACGAGAGGGACGATGTGA
TAAGTGAACATATACTTTCAGCTCTGAGAGACCCGAATGTGGTAATCGTCTAT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC100869 representing NP_044474
Red=Cloning sites Green=Tags

MAASGGEGSRDVRAPGPPPQQPGARPAVRFRDEAFLNFTSMHGVQPIIARIRELSQQQLDVTQVPRLQWF
RDVAALEVPTGLPLREFPFAAYLITGNAGSGKSTCVQTLNEVLDCVVTGATRIAAQNMYVKLSGAFLSRP
INTIFHEFGFRGNHVQAQLGQHPYTLASSPASLEDLQRRDLTYYWEVILDITKRALAAHGGEDARNEFHA
LTALEQTLGLGQGALTRLASVTHGALPAFTRSNIIVIDEAGLLGRHLLTTVVYCWWMINALYHTPQYAGR
LRPVLVCVGSPTQTASLESTFEHQKLRCSVRQSENVLTYLICNRTLREYTRLSHSWAIFINNKRCVEHEF
GNLMKVLEYGLPITEEHMQFVDRFVVPESYITNPANLPGWTRLFSSHKEVSAYMAKLHAYLKVTREGEFV
VFTLPVLTFVSVKEFDEYRRLTQQPTLTMEKWITANASRITNYSQSQDQDAGHVRCEVHSKQQLVVARND
ITYVLNSQVAVTARLRKMVFGFDGTFRTFEAVLRDDSFVKTQGETSVEFAYRFLSRLMFGGLIHFYNFLQ
RPGLDATQRTLAYGRLGELTAELLSLRRDAAGASATRAADTSDRSPGERAFNFKHLGPRDGGPDDFPDDD
LDVIFAGLDEQQLDVFYCHYALEEPETTAAVHAQFGLLKRAFLGRYLILRELFGEVFESAPFSTYVDNVI
FRGCELLTGSPRGGLMSVALQTDNYTLMGYTYTRVFAFAEELRRRHATAGVAEFLEESPLPYIVLRDQHG
FMSVVNTNISEFVESIDSTELAMAINADYGISSKLAMTITRSQGLSLDKVAICFTPGNLRLNSAYVAMSR
TTSSEFLHMNLNPLRERHERDDVISEHILSALRDPNVVIVY

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NC_001798
ORF Size 2643 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NC_001798.1, NP_044474
RefSeq ORF 2643 bp
Locus ID 1487338
MW 98.9 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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