UL9 (NC_001798) Virus Tagged ORF Clone

CAT#: VC100873

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for DNA replication origin-binding helicase [Human herpesvirus 2], codon optimized for human cell expression, NP_044478



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CNY 13,110.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 100 ul

CNY 600.00


Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
    • 100 ul

CNY 1,050.00

Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC100873 represents NCBI reference of NP_044478 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAATGTGGCGACCTGCACACACCAGACCCATCACGCTGCCAGAGCCCCCGGCGCAACATCCGCTCCTG
GGGCTGCCTCCGGGGATCCTTTGGGCGCAAGGAGGCCAATTGGAGACGACGAGTGTGAGCAGTACACCTC
AAGCGTAAGCTTGGCACGGATGCTCTACGGGGGCGACCTCGCGGAATGGGTCCCAAGAGTCCATCCTAAG
ACGACAATCGAGAGACAACAGCATGGGCCAGTGACTTTCCCCGATGCATCAGCCCCGACCGCGCGGTGTG
TTACCGTGGTGAGGGCTCCTATGGGCAGCGGCAAAACAACCGCGCTCATCCGCTGGCTCGGCGAAGCCAT
CCATTCCCCAGACACTTCTGTGCTGGTTGTATCCTGTCGGAGAAGTTTTACTCAGACGCTCGCTACCAGG
TTCGCAGAATCAGGGCTCCCTGACTTTGTGACCTATTTTTCTTCTACAAATTACATTATGAACGATCGGC
CTTTTCATAGGCTCATAGTGCAGGTCGAGTCACTGCATCGCGTAGGACCTAACCTGTTGAACAATTATGA
CGTACTGGTCCTGGATGAAGTGATGTCCACCCTGGGCCAGCTGTACAGCCCAACCATGCAGCAGCTGGGG
CGCGTGGACGCTCTGATGCTTAGACTCCTCCGAACGTGCCCTCGAATTATCGCAATGGACGCGACGGCCA
ACGCCCAGCTCGTCGACTTCCTCTGTAGTCTGAGGGGAGAAAAAAACGTCCACGTCGTTATCGGCGAGTA
CGCCATGCCTGGCTTCAGTGCTCGGCGGTGCCTGTTTCTTCCGCGACTTGGGCCTGAAGTACTGCAGGCA
GCACTGAGAAGACGGGGACCCGCTGGCGGCGCTCCCCCTCCAGACGCCCCACCCGACGCTACATTTTTTG
GGGAGCTCGAAGCTAGGCTGGCCGGCGGTGATAATGTTTGCATCTTTTCCTCCACCGTGTCTTTTGCCGA
GGTAGTTGCCAGATTCTGTCGCCAATTCACCGACAGAGTTCTGCTTTTGCATTCCTTGACTCCCCCTGGG
GACGTGACTACATGGGGAAGATACAGAGTTGTTATATACACAACCGTGGTGACCGTGGGCCTCTCATTCG
ACCCCCCTCACTTCGATTCCATGTTTGCTTACGTCAAGCCTATGAACTACGGTCCTGACATGGTGTCCGT
GTATCAGTCACTGGGGAGGGTCCGCACTCTGCGGAAAGGCGAACTGCTGATCTACATGGACGGCTCAGGT
GCTAGGAGCGAGCCTGTGTTTACCCCCATGCTCCTTAACCATGTAGTTTCTGCGTCTGGACAGTGGCCTG
CACAGTTCAGCCAGGTGACTAATCTTCTGTGCCGGCGGTTTAAGGGCAGATGCGACGCCTCACACGCGGA
CGCTGCTCAGGCCAGGGGAAGCCGAATCTACTCCAAATTCCGCTACAAGCACTATTTCGAACGCTGTACT
CTGGCATGTCTGGCCGATAGCCTGAACATTCTTCACATGCTTCTCACCCTGAATTGCATGCATGTGAGAT
TCTGGGGCCATGATGCAGCTCTTACCCCAAGGAACTTCTGTCTGTTCCTGAGGGGAATACACTTTGATGC
TCTCCGGGCGCAGCGGGACCTGCGGGAGCTTAGGTGTCAGGACCCAGACACCAGTCTTTCCGCCCAGGCA
GCCGAAACCGAGGAAGTGGGACTCTTTGTTGAGAAATATCTGCGCCCCGACGTCGCCCCAGCTGAAGTAG
TGGCCCTGATGCGAGGGTTGAACTCTCTCGTAGGCCGGACTAGGTTCATTTACCTTGTGCTTCTGGAAGC
TTGTCTTAGAGTACCAATGGCCGCCCACTCAAGCGCCATCTTCAGAAGACTTTACGACCATTACGCTACA
GGAGTCATCCCAACAATTAACGCTGCAGGCGAGCTCGAACTGGTCGCCCTGCATCCGACATTGAACGTGG
CCCCCGTTTGGGAGCTGTTTAGGTTGTGTAGTACTATGGCAGCTTGCCTTCAGTGGGATAGCATGGCCGG
TGGCTCCGGCCGCACGTTTAGCCCAGAAGATGTGCTGGAGTTGCTTAACCCCCATTATGATCGGTACATG
CAGCTGGTTTTCGAGCTGGGACACTGTAACGTCACTGACGGGCCTCTGCTGAGCGAAGATGCTGTAAAAA
GAGTCGCCGACGCCTTGTCCGGATGCCCACCAAGAGGTTCCGTGAGCGAAACAGAACACGCTCTTTCCCT
GTTTAAGATTATTTGGGGCGAACTCTTCGGAGTCCAGCTCGCCAAATCTACCCAGACCTTCCCAGGGGCC
GGAAGGGTAAAGAACCTGACCAAACGAGCTATTGTGGAACTTCTCGATGCTCATCGGATCGATCACTCAG
CCTGTCGAACCCACAGGCAACTTTATGCACTGCTGATGGCCCACAAGAGAGAGTTTGCAGGCGCCAGATT
TAAGCTCCGCGCCCCCGCTTGGGGCCGCTGCCTGCGAACCCACGCATCCGGCGCCCAGCCCAATACCGAC
ATTATCCTGGAAGCAGCCCTGTCCGAATTGCCCACTGAGGCTTGGCCCATGATGCAGGGGGCTGTAAATT
TTAGTACTCTT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC100873 representing NP_044478
Red=Cloning sites Green=Tags

MNVATCTHQTHHAARAPGATSAPGAASGDPLGARRPIGDDECEQYTSSVSLARMLYGGDLAEWVPRVHPK
TTIERQQHGPVTFPDASAPTARCVTVVRAPMGSGKTTALIRWLGEAIHSPDTSVLVVSCRRSFTQTLATR
FAESGLPDFVTYFSSTNYIMNDRPFHRLIVQVESLHRVGPNLLNNYDVLVLDEVMSTLGQLYSPTMQQLG
RVDALMLRLLRTCPRIIAMDATANAQLVDFLCSLRGEKNVHVVIGEYAMPGFSARRCLFLPRLGPEVLQA
ALRRRGPAGGAPPPDAPPDATFFGELEARLAGGDNVCIFSSTVSFAEVVARFCRQFTDRVLLLHSLTPPG
DVTTWGRYRVVIYTTVVTVGLSFDPPHFDSMFAYVKPMNYGPDMVSVYQSLGRVRTLRKGELLIYMDGSG
ARSEPVFTPMLLNHVVSASGQWPAQFSQVTNLLCRRFKGRCDASHADAAQARGSRIYSKFRYKHYFERCT
LACLADSLNILHMLLTLNCMHVRFWGHDAALTPRNFCLFLRGIHFDALRAQRDLRELRCQDPDTSLSAQA
AETEEVGLFVEKYLRPDVAPAEVVALMRGLNSLVGRTRFIYLVLLEACLRVPMAAHSSAIFRRLYDHYAT
GVIPTINAAGELELVALHPTLNVAPVWELFRLCSTMAACLQWDSMAGGSGRTFSPEDVLELLNPHYDRYM
QLVFELGHCNVTDGPLLSEDAVKRVADALSGCPPRGSVSETEHALSLFKIIWGELFGVQLAKSTQTFPGA
GRVKNLTKRAIVELLDAHRIDHSACRTHRQLYALLMAHKREFAGARFKLRAPAWGRCLRTHASGAQPNTD
IILEAALSELPTEAWPMMQGAVNFSTL

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NC_001798
ORF Size 2601 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NC_001798.1, NP_044478
RefSeq ORF 2601 bp
Locus ID 1487349
MW 95.8 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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