UL30 (NC_001798) Virus Tagged ORF Clone

CAT#: VC100895

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for DNA polymerase catalytic subunit [Human herpesvirus 2], codon optimized for human cell expression, NP_044500



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CNY 18,810.00


货期*
7周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 100 ul

CNY 600.00


Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
    • 100 ul

CNY 1,050.00

Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC100895 represents NCBI reference of NP_044500 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTTCTGTGCAGCCGGCGGACCTGCCTCCCCTGGCGGAAAACCCGCAGCACGCGCAGCCTCCGGGTTTT
TTGCACCACACAACCCAAGAGGAGCTACACAGACCGCCCCCCCGCCATGTCGCAGGCAGAATTTCTACAA
TCCGCACCTCGCCCAGACCGGCACCCAACCCAAAGCGCTCGGTCCAGCCCAGAGGCATACCTACTACTCC
GAGTGTGATGAATTTAGGTTTATCGCCCCTCGGAGCCTCGATGAGGACGCCCCTGCTGAACAGAGAACCG
GCGTCCACGACGGCCGCCTGCGGAGAGCTCCTAAAGTGTACTGCGGGGGCGATGAGCGGGATGTCTTGCG
GGTGGGGCCAGAGGGCTTTTGGCCACGCAGGCTCCGGTTGTGGGGAGGCGCTGACCACGCTCCAGAAGGC
TTCGACCCCACAGTCACTGTTTTTCACGTTTATGATATACTCGAGCACGTGGAACACGCTTATTCTATGC
GGGCTGCACAATTGCACGAGCGCTTCATGGACGCAATCACTCCAGCCGGCACAGTCATCACTCTGCTCGG
ACTTACTCCAGAGGGCCATCGCGTGGCGGTGCACGTGTACGGTACCCGCCAGTATTTTTATATGAATAAA
GCGGAGGTCGATCGGCACCTGCAATGTAGAGCCCCAAGAGATTTGTGTGAGCGGCTGGCAGCAGCACTCA
GAGAGTCACCTGGCGCTTCTTTCCGCGGCATTAGCGCTGACCACTTTGAAGCAGAGGTGGTGGAAAGGGC
TGACGTGTACTACTACGAAACCAGGCCAACACTGTACTACAGAGTGTTCGTGAGATCAGGACGCGCCCTG
GCTTACCTCTGCGACAACTTTTGCCCCGCTATTAGAAAGTATGAAGGGGGTGTGGATGCCACAACCCGCT
TTATCCTCGATAACCCTGGATTCGTCACATTTGGATGGTATCGCCTGAAACCTGGAAGAGGCAACGCTCC
TGCCCAGCCAAGGCCTCCTACAGCTTTTGGCACCAGTTCCGATGTCGAATTTAATTGCACCGCCGATAAC
CTGGCAGTCGAAGGAGCTATGTGCGATCTCCCGGCATATAAGCTTATGTGCTTCGATATAGAATGCAAAG
CAGGTGGCGAAGACGAACTGGCTTTCCCGGTGGCCGAGCGACCAGAGGATCTCGTGATTCAGATCAGCTG
TTTGCTGTACGACCTCTCAACTACCGCACTGGAACATATACTCCTGTTCAGCCTCGGATCCTGTGATCTT
CCCGAATCCCACCTGAGCGACCTCGCATCCCGGGGCTTGCCCGCACCCGTGGTGCTCGAATTCGACTCTG
AATTTGAAATGCTGCTGGCATTTATGACCTTTGTCAAACAATACGGACCAGAGTTTGTAACCGGGTATAA
TATCATCAATTTCGATTGGCCTTTTGTACTGACTAAGCTGACAGAGATCTACAAAGTGCCACTCGATGGC
TACGGAAGGATGAACGGGCGAGGAGTATTCCGAGTCTGGGATATCGGCCAGAGCCATTTTCAGAAAAGGT
CAAAAATCAAAGTTAACGGAATGGTCAATATTGATATGTACGGGATTATAACAGACAAGGTGAAGCTGAG
TAGCTATAAGCTCAACGCCGTGGCCGAGGCAGTTCTCAAAGATAAGAAAAAGGATCTCAGCTATAGAGAC
ATTCCTGCGTACTATGCTTCCGGCCCCGCTCAAAGAGGCGTTATCGGCGAATATTGTGTTCAAGACAGCC
TCCTCGTCGGCCAGCTGTTCTTCAAATTCCTGCCCCACCTGGAGTTGAGTGCCGTTGCACGCCTCGCTGG
TATCAATATAACACGAACCATCTATGACGGCCAACAGATTAGAGTTTTTACCTGTCTGCTCAGGCTGGCC
GGTCAAAAAGGTTTTATACTTCCCGACACGCAAGGCCGCTTCCGAGGGCTCGATAAGGAAGCTCCTAAAA
GGCCAGCTGTCCCCAGGGGGGAAGGGGAAAGGCCTGGCGATGGCAATGGAGATGAAGACAAGGACGACGA
CGAAGACGGGGATGAGGACGGCGATGAAAGAGAAGAGGTAGCCAGGGAGACTGGAGGCAGGCATGTTGGA
TATCAAGGCGCGAGGGTACTGGACCCCACATCTGGCTTCCATGTTGACCCTGTGGTGGTCTTCGATTTTG
CTAGCCTGTACCCATCCATTATCCAGGCACACAACCTTTGTTTCTCTACGCTCTCTCTGAGGCCAGAAGC
TGTAGCACATCTTGAGGCAGATAGGGATTACCTGGAGATAGAGGTAGGGGGCCGCCGCCTCTTTTTCGTG
AAGGCCCATGTAAGAGAAAGCCTGCTGAGCATTCTCTTGAGAGACTGGCTCGCTATGCGCAAACAGATCC
GCAGCCGAATCCCTCAATCCACCCCAGAAGAAGCGGTACTGTTGGACAAACAGCAAGCTGCTATCAAAGT
AGTCTGCAACTCTGTATACGGGTTTACCGGCGTCCAACACGGCCTCCTGCCCTGCTTGCACGTGGCTGCC
ACTGTGACAACAATTGGACGGGAAATGCTCCTTGCAACGCGGGCCTACGTACACGCCAGGTGGGCTGAAT
TCGACCAGCTTCTTGCCGATTTCCCAGAAGCCGCTGGCATGCGCGCTCCTGGGCCGTACAGTATGCGCAT
TATTTACGGGGATACGGACTCCATTTTCGTGCTGTGTAGGGGACTCACTGCCGCTGGGCTCGTCGCCATG
GGCGACAAGATGGCTAGTCACATCAGTAGGGCTCTTTTCCTCCCACCAATCAAGTTGGAGTGTGAGAAAA
CTTTTACGAAACTCCTGTTGATTGCAAAAAAGAAGTATATTGGGGTCATCTGTGGAGGCAAAATGCTGAT
CAAGGGCGTGGACCTCGTGAGAAAAAACAACTGTGCATTTATCAACAGGACCTCCCGGGCGCTCGTCGAT
CTTCTCTTCTACGACGACACCGTCTCTGGTGCCGCCGCCGCCCTGGCTGAAAGACCCGCAGAGGAGTGGT
TGGCCAGGCCCTTGCCTGAGGGCCTGCAAGCGTTCGGGGCTGTCCTTGTAGATGCTCATCGGCGCATTAC
AGATCCAGAAAGAGACATCCAGGACTTTGTTCTCACTGCGGAACTGAGTAGACACCCACGCGCCTATACA
AATAAGAGACTTGCCCATCTTACCGTGTACTATAAGCTGATGGCCCGCCGAGCGCAGGTACCTTCCATTA
AGGATCGGATCCCTTATGTCATTGTCGCGCAGACACGCGAAGTAGAGGAAACAGTAGCCCGGCTGGCTGC
CTTGCGGGAACTTGATGCCGCTGCACCAGGAGACGAGCCAGCTCCACCTGCGGCTCTCCCCTCTCCAGCA
AAGAGACCTAGAGAGACCCCCTCTCATGCAGATCCTCCAGGTGGTGCATCTAAACCCAGGAAGCTGCTGG
TTTCTGAGCTGGCTGAAGATCCTGGGTACGCCATCGCCCGGGGCGTCCCGCTGAACACTGACTACTACTT
TAGTCACCTGTTGGGGGCTGCATGTGTGACCTTCAAAGCTCTGTTCGGAAACAATGCCAAGATTACGGAG
TCTCTTTTGAAGAGGTTCATTCCAGAGACCTGGCACCCTCCAGATGATGTAGCAGCCAGACTCAGGGCAG
CAGGGTTCGGACCCGCAGGAGCGGGCGCGACCGCCGAAGAGACTAGACGCATGCTGCACAGGGCATTCGA
CACTTTGGCC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC100895 representing NP_044500
Red=Cloning sites Green=Tags

MFCAAGGPASPGGKPAARAASGFFAPHNPRGATQTAPPPCRRQNFYNPHLAQTGTQPKALGPAQRHTYYS
ECDEFRFIAPRSLDEDAPAEQRTGVHDGRLRRAPKVYCGGDERDVLRVGPEGFWPRRLRLWGGADHAPEG
FDPTVTVFHVYDILEHVEHAYSMRAAQLHERFMDAITPAGTVITLLGLTPEGHRVAVHVYGTRQYFYMNK
AEVDRHLQCRAPRDLCERLAAALRESPGASFRGISADHFEAEVVERADVYYYETRPTLYYRVFVRSGRAL
AYLCDNFCPAIRKYEGGVDATTRFILDNPGFVTFGWYRLKPGRGNAPAQPRPPTAFGTSSDVEFNCTADN
LAVEGAMCDLPAYKLMCFDIECKAGGEDELAFPVAERPEDLVIQISCLLYDLSTTALEHILLFSLGSCDL
PESHLSDLASRGLPAPVVLEFDSEFEMLLAFMTFVKQYGPEFVTGYNIINFDWPFVLTKLTEIYKVPLDG
YGRMNGRGVFRVWDIGQSHFQKRSKIKVNGMVNIDMYGIITDKVKLSSYKLNAVAEAVLKDKKKDLSYRD
IPAYYASGPAQRGVIGEYCVQDSLLVGQLFFKFLPHLELSAVARLAGINITRTIYDGQQIRVFTCLLRLA
GQKGFILPDTQGRFRGLDKEAPKRPAVPRGEGERPGDGNGDEDKDDDEDGDEDGDEREEVARETGGRHVG
YQGARVLDPTSGFHVDPVVVFDFASLYPSIIQAHNLCFSTLSLRPEAVAHLEADRDYLEIEVGGRRLFFV
KAHVRESLLSILLRDWLAMRKQIRSRIPQSTPEEAVLLDKQQAAIKVVCNSVYGFTGVQHGLLPCLHVAA
TVTTIGREMLLATRAYVHARWAEFDQLLADFPEAAGMRAPGPYSMRIIYGDTDSIFVLCRGLTAAGLVAM
GDKMASHISRALFLPPIKLECEKTFTKLLLIAKKKYIGVICGGKMLIKGVDLVRKNNCAFINRTSRALVD
LLFYDDTVSGAAAALAERPAEEWLARPLPEGLQAFGAVLVDAHRRITDPERDIQDFVLTAELSRHPRAYT
NKRLAHLTVYYKLMARRAQVPSIKDRIPYVIVAQTREVEETVARLAALRELDAAAPGDEPAPPAALPSPA
KRPRETPSHADPPGGASKPRKLLVSELAEDPGYAIARGVPLNTDYYFSHLLGAACVTFKALFGNNAKITE
SLLKRFIPETWHPPDDVAARLRAAGFGPAGAGATAEETRRMLHRAFDTLA

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NC_001798
ORF Size 3720 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NC_001798.1, NP_044500
RefSeq ORF 3720 bp
Locus ID 1487316
MW 137.3 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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