UL27 (NC_001798) Virus Tagged ORF Clone

CAT#: VC100892

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for envelope glycoprotein B [Human herpesvirus 2], codon optimized for human cell expression, NP_044497



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CNY 13,680.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 100 ul

CNY 600.00


Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
    • 100 ul

CNY 1,050.00

Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC100892 represents NCBI reference of NP_044497 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCGGGGAGGGGGACTCATCTGCGCGTTGGTCGTTGGGGCCTTGGTGGCCGCTGTTGCCTCAGCCGCCC
CAGCAGCTCCAGCAGCACCCAGAGCTTCTGGAGGAGTCGCTGCCACTGTCGCTGCCAATGGAGGACCCGC
TTCAAGACCACCTCCTGTGCCATCACCAGCAACCACAAAGGCCAGAAAGAGGAAGACGAAAAAGCCGCCA
AAACGCCCCGAGGCCACTCCCCCACCTGACGCAAACGCAACTGTGGCTGCAGGGCACGCAACCCTGCGCG
CCCACCTGAGGGAAATCAAGGTAGAGAATGCTGACGCCCAGTTCTACGTTTGCCCACCCCCTACTGGAGC
CACAGTCGTTCAGTTCGAGCAGCCTCGCAGGTGCCCAACTCGACCTGAAGGACAGAATTACACCGAGGGC
ATCGCCGTAGTCTTCAAAGAGAACATTGCCCCGTATAAGTTCAAGGCAACAATGTATTACAAAGACGTGA
CAGTGTCTCAAGTCTGGTTTGGCCACCGGTACTCCCAGTTTATGGGCATATTCGAAGATCGAGCCCCTGT
ACCTTTCGAAGAGGTGATCGACAAAATCAACACCAAAGGCGTGTGCAGATCCACTGCGAAGTACGTCAGA
AACAATATGGAAACCACAGCCTTCCATCGCGATGACCACGAAACAGACATGGAACTGAAACCAGCAAAAG
TGGCAACGAGGACCAGCCGAGGGTGGCATACAACGGATTTGAAATATAACCCCAGCAGAGTCGAAGCTTT
TCATAGATACGGGACTACTGTCAATTGTATTGTCGAGGAAGTGGATGCCAGAAGTGTCTATCCTTACGAT
GAGTTTGTGCTTGCAACTGGAGATTTTGTTTATATGTCCCCATTTTACGGGTATCGCGAAGGATCCCACA
CCGAACATACTAGCTACGCCGCTGACCGGTTTAAACAAGTGGACGGATTCTATGCTCGGGACCTGACTAC
GAAAGCTCGCGCTACCAGCCCTACCACAAGAAATTTGCTTACCACGCCCAAGTTCACTGTTGCTTGGGAT
TGGGTTCCCAAACGACCTGCAGTGTGCACAATGACCAAGTGGCAGGAGGTCGACGAGATGCTGCGCGCCG
AGTACGGGGGATCTTTCCGGTTCTCTTCAGACGCAATTTCCACGACGTTTACCACTAACCTGACAGAGTA
CTCACTGAGCAGGGTGGATCTTGGCGACTGTATTGGGAGAGATGCGCGCGAGGCTATTGACCGCATGTTC
GCCCGCAAATATAACGCCACCCACATAAAGGTTGGGCAGCCACAATATTACTTGGCCACAGGCGGGTTTC
TGATTGCTTACCAACCTCTGCTGTCCAACACCCTGGCAGAGTTGTACGTAAGGGAGTACATGAGGGAACA
GGACCGGAAACCACGCAACGCTACCCCAGCTCCACTCCGCGAGGCACCCTCTGCCAATGCATCTGTAGAA
CGCATTAAAACAACCAGCTCCATTGAATTCGCGCGGCTTCAGTTCACCTACAATCACATCCAAAGACACG
TGAACGACATGCTTGGCAGGATCGCGGTCGCCTGGTGCGAGCTGCAGAACCACGAGCTGACTCTCTGGAA
TGAAGCGAGGAAACTCAACCCGAACGCCATAGCCTCCGCGACTGTGGGAAGGCGAGTGTCAGCCAGGATG
CTGGGCGACGTGATGGCCGTGTCTACTTGCGTCCCTGTGGCCCCTGACAACGTTATCGTACAAAATAGCA
TGAGGGTGTCTAGCAGACCAGGCACTTGCTACTCTCGACCCCTCGTGAGCTTCAGGTATGAGGATCAGGG
GCCATTGATAGAGGGGCAACTCGGCGAGAACAACGAGCTGAGACTGACGAGAGATGCTCTGGAGCCCTGC
ACAGTCGGGCACCGCAGATATTTTATTTTTGGCGGGGGGTATGTCTACTTCGAGGAATACGCTTATAGCC
ACCAGCTTAGCCGCGCAGACGTCACCACCGTGTCTACCTTTATCGATCTGAATATAACTATGCTGGAGGA
TCACGAATTCGTGCCTCTGGAAGTGTATACCAGACATGAGATCAAAGACAGCGGGCTCCTTGACTATACC
GAAGTGCAGAGACGGAACCAGCTCCACGACCTGCGGTTTGCAGATATAGATACCGTGATCCGCGCTGATG
CTAATGCGGCTATGTTTGCAGGACTGTGCGCTTTTTTTGAAGGGATGGGTGACCTTGGCCGCGCAGTTGG
CAAGGTCGTGATGGGGGTAGTCGGCGGGGTGGTCTCAGCTGTAAGTGGAGTCAGTTCTTTTATGAGCAAC
CCATTTGGCGCTCTCGCCGTAGGTCTGCTTGTGCTCGCTGGCCTGGTCGCAGCCTTTTTTGCGTTTCGCT
ATGTCCTGCAGTTGCAGCGAAATCCTATGAAGGCACTTTACCCCCTGACCACAAAGGAATTGAAGACCTC
TGACCCTGGAGGAGTAGGGGGCGAGGGCGAGGAAGGCGCTGAGGGCGGCGGGTTTGATGAGGCTAAGCTG
GCCGAAGCAAGAGAGATGATAAGATATATGGCACTGGTAAGCGCTATGGAGAGGACAGAACATAAGGCAC
GAAAGAAAGGGACCAGCGCTCTTTTGAGTTCCAAAGTGACCAACATGGTCCTGCGCAAAAGAAATAAGGC
GCGGTATAGCCCGCTCCACAACGAGGACGAGGCAGGAGATGAAGATGAACTG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC100892 representing NP_044497
Red=Cloning sites Green=Tags

MRGGGLICALVVGALVAAVASAAPAAPAAPRASGGVAATVAANGGPASRPPPVPSPATTKARKRKTKKPP
KRPEATPPPDANATVAAGHATLRAHLREIKVENADAQFYVCPPPTGATVVQFEQPRRCPTRPEGQNYTEG
IAVVFKENIAPYKFKATMYYKDVTVSQVWFGHRYSQFMGIFEDRAPVPFEEVIDKINTKGVCRSTAKYVR
NNMETTAFHRDDHETDMELKPAKVATRTSRGWHTTDLKYNPSRVEAFHRYGTTVNCIVEEVDARSVYPYD
EFVLATGDFVYMSPFYGYREGSHTEHTSYAADRFKQVDGFYARDLTTKARATSPTTRNLLTTPKFTVAWD
WVPKRPAVCTMTKWQEVDEMLRAEYGGSFRFSSDAISTTFTTNLTEYSLSRVDLGDCIGRDAREAIDRMF
ARKYNATHIKVGQPQYYLATGGFLIAYQPLLSNTLAELYVREYMREQDRKPRNATPAPLREAPSANASVE
RIKTTSSIEFARLQFTYNHIQRHVNDMLGRIAVAWCELQNHELTLWNEARKLNPNAIASATVGRRVSARM
LGDVMAVSTCVPVAPDNVIVQNSMRVSSRPGTCYSRPLVSFRYEDQGPLIEGQLGENNELRLTRDALEPC
TVGHRRYFIFGGGYVYFEEYAYSHQLSRADVTTVSTFIDLNITMLEDHEFVPLEVYTRHEIKDSGLLDYT
EVQRRNQLHDLRFADIDTVIRADANAAMFAGLCAFFEGMGDLGRAVGKVVMGVVGGVVSAVSGVSSFMSN
PFGALAVGLLVLAGLVAAFFAFRYVLQLQRNPMKALYPLTTKELKTSDPGGVGGEGEEGAEGGGFDEAKL
AEAREMIRYMALVSAMERTEHKARKKGTSALLSSKVTNMVLRKRNKARYSPLHNEDEAGDEDEL

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NC_001798
ORF Size 2712 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NC_001798.1, NP_044497
RefSeq ORF 2712 bp
Locus ID 1487312
MW 100.2 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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