UL27 (NC_001798) Virus Tagged ORF Clone
CAT#: VC100892
- TrueORF®
Myc-DDK-tagged ORF clone of viral ORF for envelope glycoprotein B [Human herpesvirus 2], codon optimized for human cell expression, NP_044497
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CNY 13,680.00
Product images
CNY 600.00
CNY 1,050.00
Specifications
Product Data | |
Type | Virus Tagged ORF Clone |
Tag | Myc-DDK |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>The Viral ORF clone VC100892 represents NCBI reference of NP_044497 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s) GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGGGGAGGGGGACTCATCTGCGCGTTGGTCGTTGGGGCCTTGGTGGCCGCTGTTGCCTCAGCCGCCC CAGCAGCTCCAGCAGCACCCAGAGCTTCTGGAGGAGTCGCTGCCACTGTCGCTGCCAATGGAGGACCCGC TTCAAGACCACCTCCTGTGCCATCACCAGCAACCACAAAGGCCAGAAAGAGGAAGACGAAAAAGCCGCCA AAACGCCCCGAGGCCACTCCCCCACCTGACGCAAACGCAACTGTGGCTGCAGGGCACGCAACCCTGCGCG CCCACCTGAGGGAAATCAAGGTAGAGAATGCTGACGCCCAGTTCTACGTTTGCCCACCCCCTACTGGAGC CACAGTCGTTCAGTTCGAGCAGCCTCGCAGGTGCCCAACTCGACCTGAAGGACAGAATTACACCGAGGGC ATCGCCGTAGTCTTCAAAGAGAACATTGCCCCGTATAAGTTCAAGGCAACAATGTATTACAAAGACGTGA CAGTGTCTCAAGTCTGGTTTGGCCACCGGTACTCCCAGTTTATGGGCATATTCGAAGATCGAGCCCCTGT ACCTTTCGAAGAGGTGATCGACAAAATCAACACCAAAGGCGTGTGCAGATCCACTGCGAAGTACGTCAGA AACAATATGGAAACCACAGCCTTCCATCGCGATGACCACGAAACAGACATGGAACTGAAACCAGCAAAAG TGGCAACGAGGACCAGCCGAGGGTGGCATACAACGGATTTGAAATATAACCCCAGCAGAGTCGAAGCTTT TCATAGATACGGGACTACTGTCAATTGTATTGTCGAGGAAGTGGATGCCAGAAGTGTCTATCCTTACGAT GAGTTTGTGCTTGCAACTGGAGATTTTGTTTATATGTCCCCATTTTACGGGTATCGCGAAGGATCCCACA CCGAACATACTAGCTACGCCGCTGACCGGTTTAAACAAGTGGACGGATTCTATGCTCGGGACCTGACTAC GAAAGCTCGCGCTACCAGCCCTACCACAAGAAATTTGCTTACCACGCCCAAGTTCACTGTTGCTTGGGAT TGGGTTCCCAAACGACCTGCAGTGTGCACAATGACCAAGTGGCAGGAGGTCGACGAGATGCTGCGCGCCG AGTACGGGGGATCTTTCCGGTTCTCTTCAGACGCAATTTCCACGACGTTTACCACTAACCTGACAGAGTA CTCACTGAGCAGGGTGGATCTTGGCGACTGTATTGGGAGAGATGCGCGCGAGGCTATTGACCGCATGTTC GCCCGCAAATATAACGCCACCCACATAAAGGTTGGGCAGCCACAATATTACTTGGCCACAGGCGGGTTTC TGATTGCTTACCAACCTCTGCTGTCCAACACCCTGGCAGAGTTGTACGTAAGGGAGTACATGAGGGAACA GGACCGGAAACCACGCAACGCTACCCCAGCTCCACTCCGCGAGGCACCCTCTGCCAATGCATCTGTAGAA CGCATTAAAACAACCAGCTCCATTGAATTCGCGCGGCTTCAGTTCACCTACAATCACATCCAAAGACACG TGAACGACATGCTTGGCAGGATCGCGGTCGCCTGGTGCGAGCTGCAGAACCACGAGCTGACTCTCTGGAA TGAAGCGAGGAAACTCAACCCGAACGCCATAGCCTCCGCGACTGTGGGAAGGCGAGTGTCAGCCAGGATG CTGGGCGACGTGATGGCCGTGTCTACTTGCGTCCCTGTGGCCCCTGACAACGTTATCGTACAAAATAGCA TGAGGGTGTCTAGCAGACCAGGCACTTGCTACTCTCGACCCCTCGTGAGCTTCAGGTATGAGGATCAGGG GCCATTGATAGAGGGGCAACTCGGCGAGAACAACGAGCTGAGACTGACGAGAGATGCTCTGGAGCCCTGC ACAGTCGGGCACCGCAGATATTTTATTTTTGGCGGGGGGTATGTCTACTTCGAGGAATACGCTTATAGCC ACCAGCTTAGCCGCGCAGACGTCACCACCGTGTCTACCTTTATCGATCTGAATATAACTATGCTGGAGGA TCACGAATTCGTGCCTCTGGAAGTGTATACCAGACATGAGATCAAAGACAGCGGGCTCCTTGACTATACC GAAGTGCAGAGACGGAACCAGCTCCACGACCTGCGGTTTGCAGATATAGATACCGTGATCCGCGCTGATG CTAATGCGGCTATGTTTGCAGGACTGTGCGCTTTTTTTGAAGGGATGGGTGACCTTGGCCGCGCAGTTGG CAAGGTCGTGATGGGGGTAGTCGGCGGGGTGGTCTCAGCTGTAAGTGGAGTCAGTTCTTTTATGAGCAAC CCATTTGGCGCTCTCGCCGTAGGTCTGCTTGTGCTCGCTGGCCTGGTCGCAGCCTTTTTTGCGTTTCGCT ATGTCCTGCAGTTGCAGCGAAATCCTATGAAGGCACTTTACCCCCTGACCACAAAGGAATTGAAGACCTC TGACCCTGGAGGAGTAGGGGGCGAGGGCGAGGAAGGCGCTGAGGGCGGCGGGTTTGATGAGGCTAAGCTG GCCGAAGCAAGAGAGATGATAAGATATATGGCACTGGTAAGCGCTATGGAGAGGACAGAACATAAGGCAC GAAAGAAAGGGACCAGCGCTCTTTTGAGTTCCAAAGTGACCAACATGGTCCTGCGCAAAAGAAATAAGGC GCGGTATAGCCCGCTCCACAACGAGGACGAGGCAGGAGATGAAGATGAACTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >VC100892 representing NP_044497
Red=Cloning sites Green=Tags MRGGGLICALVVGALVAAVASAAPAAPAAPRASGGVAATVAANGGPASRPPPVPSPATTKARKRKTKKPP KRPEATPPPDANATVAAGHATLRAHLREIKVENADAQFYVCPPPTGATVVQFEQPRRCPTRPEGQNYTEG IAVVFKENIAPYKFKATMYYKDVTVSQVWFGHRYSQFMGIFEDRAPVPFEEVIDKINTKGVCRSTAKYVR NNMETTAFHRDDHETDMELKPAKVATRTSRGWHTTDLKYNPSRVEAFHRYGTTVNCIVEEVDARSVYPYD EFVLATGDFVYMSPFYGYREGSHTEHTSYAADRFKQVDGFYARDLTTKARATSPTTRNLLTTPKFTVAWD WVPKRPAVCTMTKWQEVDEMLRAEYGGSFRFSSDAISTTFTTNLTEYSLSRVDLGDCIGRDAREAIDRMF ARKYNATHIKVGQPQYYLATGGFLIAYQPLLSNTLAELYVREYMREQDRKPRNATPAPLREAPSANASVE RIKTTSSIEFARLQFTYNHIQRHVNDMLGRIAVAWCELQNHELTLWNEARKLNPNAIASATVGRRVSARM LGDVMAVSTCVPVAPDNVIVQNSMRVSSRPGTCYSRPLVSFRYEDQGPLIEGQLGENNELRLTRDALEPC TVGHRRYFIFGGGYVYFEEYAYSHQLSRADVTTVSTFIDLNITMLEDHEFVPLEVYTRHEIKDSGLLDYT EVQRRNQLHDLRFADIDTVIRADANAAMFAGLCAFFEGMGDLGRAVGKVVMGVVGGVVSAVSGVSSFMSN PFGALAVGLLVLAGLVAAFFAFRYVLQLQRNPMKALYPLTTKELKTSDPGGVGGEGEEGAEGGGFDEAKL AEAREMIRYMALVSAMERTEHKARKKGTSALLSSKVTNMVLRKRNKARYSPLHNEDEAGDEDEL TRTRRLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
ACCN | NC_001798 |
ORF Size | 2712 bp |
OTI Disclaimer | The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NC_001798.1, NP_044497 |
RefSeq ORF | 2712 bp |
Locus ID | 1487312 |
MW | 100.2 kDa |
Documents
Product Manuals |
FAQs |
SDS |
Resources
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