UL29 (NC_001798) Virus Tagged ORF Clone

CAT#: VC100894

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for single-stranded DNA-binding protein [Human herpesvirus 2], codon optimized for human cell expression, NP_044499



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CNY 18,050.00


货期*
6周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 100 ul

CNY 600.00


Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
    • 100 ul

CNY 1,050.00

Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC100894 represents NCBI reference of NP_044499 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGACACGAAGCCTAAGACCACTACCACAGTAAAAGTGCCTCCTGGGCCGATGGGCTACGTGTATGGTA
GGGCCTGTCCCGCAGAGGGCCTGGAGCTGCTTTCTCTGCTGTCCGCCAGGAGTGGCGATGCCGATGTTGC
TGTCGCCCCCCTGATTGTAGGCCTCACCGTGGAGTCCGGGTTTGAGGCCAATGTGGCTGCCGTCGTGGGG
AGCAGAACTACCGGTCTCGGAGGGACGGCAGTCTCTCTCAAGTTGATGCCTAGTCATTACAGCCCCTCAG
TGTACGTTTTCCACGGCGGTCGGCACCTTGCTCCTTCTACCCAGGCTCCAAATCTCACCCGCCTCTGTGA
GAGAGCCAGACCACATTTCGGGTTTGCCGACTATGCTCCACGGCCATGCGATCTTAAGCACGAAACAACC
GGAGACGCACTGTGCGAGAGACTGGGCCTTGACCCTGACCGAGCCCTCCTGTACCTCGTGATCACTGAAG
GCTTCAGAGAAGCCGTATGTATCAGCAACACCTTCCTGCATCTTGGTGGCATGGATAAAGTCACCATAGG
CGACGCAGAAGTGCACAGGATCCCCGTATATCCTCTGCAAATGTTTATGCCTGATTTTTCTCGGGTAATC
GCCGACCCATTCAACTGTAACCATCGGAGCATCGGGGAGAATTTTAATTATCCTCTGCCCTTTTTCAATC
GCCCTCTTGCAAGGCTTCTCTTCGAAGCCGTCGTGGGTCCGGCCGCTGTTGCACTTAGAGCCAGGAACGT
CGACGCCGTGGCAAGAGCAGCTGCACACCTGGCGTTTGATGAGAACCATGAGGGCGCTGCTCTTCCTGCT
GATATTACATTCACAGCATTCGAAGCCTCCCAGGGAAAGCCTCAGAGAGGGGCTAGAGACGCTGGCAACA
AGGGTCCAGCGGGGGGATTCGAGCAACGGCTCGCCTCTGTTATGGCAGGGGACGCTGCACTGGCCCTTGA
GAGCATCGTGAGCATGGCCGTGTTTGACGAGCCTCCACCAGACATTACCACCTGGCCTCTTTTGGAAGGC
CAAGAGACCCCTGCCGCGAGAGCCGGGGCTGTGGGCGCCTATCTCGCCCGGGCAGCTGGCCTGGTGGGGG
CAATGGTGTTTAGTACCAACAGCGCCCTGCACCTGACTGAAGTGGATGACGCAGGCCCTGCTGACCCAAA
AGACCACTCTAAGCCTTCATTTTACAGGTTTTTCTTGGTGCCCGGAACCCATGTCGCCGCCAACCCACAG
CTGGACCGGGAGGGTCATGTGGTGCCTGGGTATGAGGGAAGGCCAACCGCCCCCCTCGTGGGAGGGACAC
AGGAGTTTGCCGGGGAGCATCTGGCAATGTTGTGTGGCTTTAGCCCAGCCTTGCTGGCTAAGATGCTGTT
TTACCTGGAACGCTGCGATGGTGGGGTCATTGTCGGTCGCCAGGAAATGGACGTGTTTCGCTACGTCGCA
GATTCCGGCCAGACCGACGTTCCCTGTAATCTGTGTACCTTTGAGACGCGACATGCTTGTGCGCACACAA
CCCTCATGAGGCTGCGCGCTAGACACCCAAAGTTCGCCTCAGCGGCACGGGGGGCAATTGGGGTGTTTGG
CACGATGAACAGCGCTTATTCTGATTGCGACGTTCTGGGTAATTACGCCGCTTTCTCCGCTCTTAAGCGC
GCTGATGGGTCAGAGAACACCCGGACAATCATGCAGGAGACCTATCGCGCTGCAACTGAAAGGGTAATGG
CCGAGCTGGAGGCACTGCAATACGTTGACCAGGCTGTACCCACTGCGCTCGGAAGACTTGAGACCATCAT
TGGGAATCGCGAAGCCCTGCATACAGTTGTGAACAATATCAAACAGCTCGTTGATCGGGAAGTAGAACAG
CTGATGCGGAACCTCATCGAGGGCAGAAATTTTAAGTTCAGAGACGGCCTCGCTGAGGCCAACCACGCTA
TGAGCCTTAGCCTGGACCCATACACATGTGGCCCTTGTCCCCTTCTGCAGCTGCTGGCAAGAAGATCAAA
CCTGGCTGTCTATCAGGACTTGGCTCTCTCCCAGTGTCATGGCGTGTTTGCTGGTCAGAGTGTCGAAGGC
CGGAATTTTCGAAATCAGTTCCAGCCCGTGCTGAGAAGGCGAGTCATGGATCTCTTTAACAACGGATTCC
TGAGCGCGAAAACACTGACTGTTGCTTTGTCTGAAGGGGCCGCCATCTGTGCGCCCAGCCTGACAGCTGG
ACAAACCGCACCTGCTGAATCCAGCTTTGAGGGCGATGTTGCGCGGGTAACATTGGGATTTCCTAAGGAA
CTTAGGGTCAAGTCTAGGGTGCTCTTCGCTGGCGCAAGCGCCAACGCTAGTGAGGCAGCCAAGGCCCGCG
TTGCATCCCTCCAGTCTGCCTACCAGAAACCCGACAAACGAGTTGATATTTTGCTTGGCCCGCTTGGTTT
CCTCCTGAAGCAGTTCCACGCCGTGATATTCCCCAATGGTAAGCCGCCAGGCAGTAACCAGCCAAACCCC
CAGTGGTTCTGGACCGCTCTCCAGCGCAACCAGCTGCCTGCACGGCTCCTGAGCCGCGAGGATATAGAGA
CAATTGCTTTTATCAAAAGATTTAGCCTCGACTACGGCGCAATAAACTTTATTAATCTTGCCCCTAATAA
CGTGTCTGAGCTGGCTATGTACTATATGGCCAATCAGATTCTGAGATATTGCGACCATAGCACCTACTTC
ATCAACACTCTGACAGCCGTGATCGCCGGCTCCAGACGGCCTCCTTCTGTTCAAGCCGCCGCTGCCTGGG
CTCCACAAGGTGGAGCCGGTCTGGAAGCAGGAGCTCGGGCACTTATGGACTCACTCGACGCTCATCCCGG
CGCCTGGACTTCAATGTTTGCAAGCTGTAACCTGCTCCGACCTGTGATGGCTGCACGGCCGATGGTGGTG
TTGGGGCTGTCCATTAGTAAGTATTATGGCATGGCCGGAAACGATAGGGTCTTTCAAGCTGGAAACTGGG
CTAGCCTGCTGGGAGGCAAGAATGCTTGTCCATTGCTGATTTTTGACCGAACGCGGAAGTTTGTCCTCGC
TTGTCCAAGGGCCGGGTTCGTCTGTGCCGCCTCATCCTTGGGCGGCGGCGCCCATGAGCACTCCCTTTGT
GAACAGCTGAGAGGGATTATTGCCGAGGGAGGGGCCGCAGTGGCATCCAGCGTGTTTGTGGCTACTGTTA
AATCACTTGGTCCTAGAACCCAGCAACTCCAGATTGAAGACTGGCTGGCCCTTTTGGAAGATGAATACCT
GTCAGAGGAAATGATGGAGTTCACGACAAGAGCTCTGGAACGGGGCCACGGCGAGTGGAGTACCGACGCC
GCCCTTGAGGTCGCTCACGAAGCTGAGGCCTTGGTGAGCCAGCTTGGGGCTGCCGGCGAGGTCTTCAACT
TTGGAGATTTCGGCGACGAAGATGACCATGCCGCAAGTTTTGGGGGACTGGCCGCGGCAGCAGGCGCCGC
AGGTGTGGCCCGAAAACGCGCTTTCCACGGCGATGATCCTTTCGGGGAGGGGCCACCTGAAAAGAAAGAC
TTGACTTTGGACATGCTG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC100894 representing NP_044499
Red=Cloning sites Green=Tags

MDTKPKTTTTVKVPPGPMGYVYGRACPAEGLELLSLLSARSGDADVAVAPLIVGLTVESGFEANVAAVVG
SRTTGLGGTAVSLKLMPSHYSPSVYVFHGGRHLAPSTQAPNLTRLCERARPHFGFADYAPRPCDLKHETT
GDALCERLGLDPDRALLYLVITEGFREAVCISNTFLHLGGMDKVTIGDAEVHRIPVYPLQMFMPDFSRVI
ADPFNCNHRSIGENFNYPLPFFNRPLARLLFEAVVGPAAVALRARNVDAVARAAAHLAFDENHEGAALPA
DITFTAFEASQGKPQRGARDAGNKGPAGGFEQRLASVMAGDAALALESIVSMAVFDEPPPDITTWPLLEG
QETPAARAGAVGAYLARAAGLVGAMVFSTNSALHLTEVDDAGPADPKDHSKPSFYRFFLVPGTHVAANPQ
LDREGHVVPGYEGRPTAPLVGGTQEFAGEHLAMLCGFSPALLAKMLFYLERCDGGVIVGRQEMDVFRYVA
DSGQTDVPCNLCTFETRHACAHTTLMRLRARHPKFASAARGAIGVFGTMNSAYSDCDVLGNYAAFSALKR
ADGSENTRTIMQETYRAATERVMAELEALQYVDQAVPTALGRLETIIGNREALHTVVNNIKQLVDREVEQ
LMRNLIEGRNFKFRDGLAEANHAMSLSLDPYTCGPCPLLQLLARRSNLAVYQDLALSQCHGVFAGQSVEG
RNFRNQFQPVLRRRVMDLFNNGFLSAKTLTVALSEGAAICAPSLTAGQTAPAESSFEGDVARVTLGFPKE
LRVKSRVLFAGASANASEAAKARVASLQSAYQKPDKRVDILLGPLGFLLKQFHAVIFPNGKPPGSNQPNP
QWFWTALQRNQLPARLLSREDIETIAFIKRFSLDYGAINFINLAPNNVSELAMYYMANQILRYCDHSTYF
INTLTAVIAGSRRPPSVQAAAAWAPQGGAGLEAGARALMDSLDAHPGAWTSMFASCNLLRPVMAARPMVV
LGLSISKYYGMAGNDRVFQAGNWASLLGGKNACPLLIFDRTRKFVLACPRAGFVCAASSLGGGAHEHSLC
EQLRGIIAEGGAAVASSVFVATVKSLGPRTQQLQIEDWLALLEDEYLSEEMMEFTTRALERGHGEWSTDA
ALEVAHEAEALVSQLGAAGEVFNFGDFGDEDDHAASFGGLAAAAGAAGVARKRAFHGDDPFGEGPPEKKD
LTLDML

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NC_001798
ORF Size 3588 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NC_001798.1, NP_044499
RefSeq ORF 3588 bp
Locus ID 1487314
MW 128.4 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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