UL37 (NC_001798) Virus Tagged ORF Clone
CAT#: VC100902
- TrueORF®
Myc-DDK-tagged ORF clone of viral ORF for tegument protein UL37 [Human herpesvirus 2], codon optimized for human cell expression, NP_044507
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CNY 16,820.00
Product images
CNY 600.00
CNY 1,050.00
Specifications
Product Data | |
Type | Virus Tagged ORF Clone |
Tag | Myc-DDK |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>The Viral ORF clone VC100902 represents NCBI reference of NP_044507 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s) GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTGACAGTGCTCTGCAGGTGCCCGCCCCCGCCGGCATGACTCCACCATCTGCACCTCCACCTAACG GCCCTCTCCAGGTTCTGCTTGGGTCTCTTACAAATCTGAGGCGACCTCCAAGTCCTTCTAGTGAGCCAGC GGGGAGTGCTGATGAGCCTGCGTTTCTCTCAGCTGCTAAGCTCCATGCCGCTACAGCTGCCTTCCTCCTG TCAGGCGCCGCCGTCGGACCAGCCGAGGCACGAGCATGCTGGCATCCACTGCTGGAACAGCTGTGCGCTC TGCATAGAGCTCACGGGCTTCCTGAAACGGCACTGTTGGCCGAAAATCTGCCGGGTTTGCTGGTGCACCG CATGGCAGTGGCTCTGCCCGAGACTCCCGAAGCCGCTTTCAGAGAAATGGATGTCATCAAGGACACCGTC TTGGCCATTACAGGCTCAGACACTACTCACGCCTTGGAGGCGGCCGGCCTCAGGACCACCGCCGCCCTGG GTCCCGTTAGAGTCAGGCAGTGCGCTGTGGAGTGGATCGATAGGTGGAGGACAGTTACACAGTCCTGTTT GGCAATGAACCCACGCACATCACTCGAAGCTCTGGGCGAGATGTCTCTGAAAATGTCACCCGTCCCTCTG GGTCAGCCTGGCGCTAATCTGACTACCCCGGCATACTCTCTGCTCTTCCCTTCACCAATTGTGCAAGAAG GACTCCGATTCCTGGCCCTGGTTAGCAACTGGGTTACACTGTTTAGCGCACACTTGCAGCGAATTGACGA TGCAGCCCTGACCCCACTCACACGCGCCTTGTTTACACTGGCCCTGGTCGACGACTATCTCACAACACCG GATAGAGGCGCCGTGGTACCTCCACCTCTGCTGGCCCAGTTTCAGCATACCGTCCGAGAGATCGATCCCG CAATTATGATTCCTCCCCTGGAGGCAACGAAGATGGTGCGCTCTCGGGAGGAGGTGCGGGTGTCAACTGC CTTGAGTAGAGTCAGTCCACGGTCTGCTTGCGCCCCGCCTGGAACGCTCATGGCTCGCGTCAGAACTGAT GCCGCCGTGTTTGACCCTGACGTTCCATTTCTCTCAGCTTCCGCCCTTGCCATATTCAGACCCGCTGTGA CAGGATTGCTCCAGCTGGGAGAACCGCCGTCAGCCGGCGCCCAGCAGCGGCTGTTGGCGCTCCTTCAGCA GACCTGGGCTCTGGTACAGAACAGCAATTCTCCCAGCGTGGTTATCAACACCTTGACAGACGCTGGGTTT ACACCAGCCCATTGTACGCAATATATTAGTGCGTTGGAGGGCTTCCTGGTTGCCGGAGTCCCTGCCAGGA CCCCCCCCGGTCACGGGCTCTCCGAGATTCAGCAACTGTTTGGATGCATCGCCCTGGCCGGAGCAAACGT CTTTGGACTCGCCCGGGAATACGGACATTACGCAGGCTACGTGAAAACCTTTCGAAGGATACAGGGTGCC TCCGAGCACACACACGGCAGACTGTGCGAAGCTGTCGGACTTTCAGGCGGGGTGCTGAGCCAGACTCTGG CTCGAATTATGGGACCTGCCGTGCCAACCGAACATCTGGCCAGTTTGAGGAGGACCCTTGTAGGAGAATT CGAAACTGCCGAGAGAAGGTTCAGCGCGGGGCAGCCAAGTCTTCTGAGAGAGACCGCCCTGATATGGCTC GATGTTTACGGCCAGACTCACTGGGATTTGACTCCTACCACACCTGCCACACCCCTCTCCGCACTTCTGC CGGTGGGGCCACCTAGTCACGCCCCTAGCGTCCATTTGGCAGCCGCCACTAAGATACGGTTCCCCGCACT GGAAGGGATCCACCCAAACGTCCTGGCCGATCCTGGTTTTGTTCCATATGTCCTGGCCCTTGTAGTGGGT GATGCTCTGCGGGCCACATGCAACGCCGCTTATCTGCCAAGACCTATTGAGTTCGCCCTGCGGGTACTGG CTTGGGCGAGGGATTTCGGCCTTGGATACCTCCCAACAGTCGAGGGCCACAGGACAAAACTTGGCGCCCT GATCACCCTCCTGGAGCCCGCAACCCGGGCCGGCGTGGGGCCAACTATGCAAATGGCCGACAACATCGAG CAGTTGCTGAGGGAGCTGTATGTTATCGCTAGAGGGGCTGTGGAGCAACTGAGACCAGCTGTGCAACTCC CACCTCCTCAGCCGCCCGAGGTGGGAAGTAGTCTCCTTCTGATCTCCATGTACGCCCTGGCCGCGCGGGG AGTTTTGCAGGAGTTGGCTGAGCGGGCTGATCCCCTCGTAAGACAATTGGAGGATGCGATCGTCCTTCTT AGGCTGCACATGCGAACACTTGCTGCATTCTTCGAATGCCGGTTTGAGTCTGACGGGCATCGACTCTACG CGGTGGTTGCCGATGCTCATGAGCGGTTGGGACCTTGGCGCCCGGAGGCTATGGGGGATGCCGTCTCACA GTATTGTGGCATGTATCACGACGCCAAGCGAGCTCTGGTCGCTAGTCTCGCCGGCCTGCGGAGCGTGGTG ACAGAGACAACAGCTCACCTCGGGGTGTGCGACGAGCTGGCCGCCCAAGTGTCACACGAGGGAAATGTCC TGGCAGTGGTTCGGCGAGAAATCCACGGTTTTCTGGCCATCGTGTCAGGTATCCATGCCCGCGCAAGCAA GTTGATGAGCGGAGACCAGGTACCTGGATTTTGCTACATGTCCCAATTTCTGGCCAGGTGGAGACGACTC AGTGCTGGGTATCAGGCAGCCCGGGCGGCCACCGGCCCCGAGCGGGTAGCCGAGTTCGTGCAGGAGTTGC ACGATACTTGGAAGGGACTGCAGACCGAGCGAGCACTGGTTGTCGCCAGATTTGCCTCAAGCGCAGACCA GAGAACAGCCGCCATCCAAGAGGTCATGGCACACGCAACAGAGGACGCACCTCCAAGTCCCGCCGCTGAT CTTGTCGTGTTGACCAACCGGCACGATTTGGGAGCCTGGGGCGATTATAGTCTGGGTCCCCTGGGGCAGC CTACGGTGGTGCCTGACAGCGTGGATCTTAGCCCCCAAGGACTCGCTGCCACCCTTTCCATGGACTGGTT GTTGATCAATGAGCTCCTCCAGGTTACGGACGGTGTCTTCAGGGCCAGTGCTTTTCGACCATCCGCTGGC CCAGGAGCGCCTGGCGATCTCGAAGCTCAAGATGCAGGCGGGTCCACTCCCGAACCTACAACACCTGGCC CCCAGGATACCCAAGCAAGGGCACCATCTACCAGACCCGCCGGGAGAGAAACTGTGCCATGGCCTAACAC CCCCGTAGAGGATGACGAGATGACACCCCAGGAGACCCCACCCGTCCACCCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >VC100902 representing NP_044507
Red=Cloning sites Green=Tags MSDSALQVPAPAGMTPPSAPPPNGPLQVLLGSLTNLRRPPSPSSEPAGSADEPAFLSAAKLHAATAAFLL SGAAVGPAEARACWHPLLEQLCALHRAHGLPETALLAENLPGLLVHRMAVALPETPEAAFREMDVIKDTV LAITGSDTTHALEAAGLRTTAALGPVRVRQCAVEWIDRWRTVTQSCLAMNPRTSLEALGEMSLKMSPVPL GQPGANLTTPAYSLLFPSPIVQEGLRFLALVSNWVTLFSAHLQRIDDAALTPLTRALFTLALVDDYLTTP DRGAVVPPPLLAQFQHTVREIDPAIMIPPLEATKMVRSREEVRVSTALSRVSPRSACAPPGTLMARVRTD AAVFDPDVPFLSASALAIFRPAVTGLLQLGEPPSAGAQQRLLALLQQTWALVQNSNSPSVVINTLTDAGF TPAHCTQYISALEGFLVAGVPARTPPGHGLSEIQQLFGCIALAGANVFGLAREYGHYAGYVKTFRRIQGA SEHTHGRLCEAVGLSGGVLSQTLARIMGPAVPTEHLASLRRTLVGEFETAERRFSAGQPSLLRETALIWL DVYGQTHWDLTPTTPATPLSALLPVGPPSHAPSVHLAAATKIRFPALEGIHPNVLADPGFVPYVLALVVG DALRATCNAAYLPRPIEFALRVLAWARDFGLGYLPTVEGHRTKLGALITLLEPATRAGVGPTMQMADNIE QLLRELYVIARGAVEQLRPAVQLPPPQPPEVGSSLLLISMYALAARGVLQELAERADPLVRQLEDAIVLL RLHMRTLAAFFECRFESDGHRLYAVVADAHERLGPWRPEAMGDAVSQYCGMYHDAKRALVASLAGLRSVV TETTAHLGVCDELAAQVSHEGNVLAVVRREIHGFLAIVSGIHARASKLMSGDQVPGFCYMSQFLARWRRL SAGYQAARAATGPERVAEFVQELHDTWKGLQTERALVVARFASSADQRTAAIQEVMAHATEDAPPSPAAD LVVLTNRHDLGAWGDYSLGPLGQPTVVPDSVDLSPQGLAATLSMDWLLINELLQVTDGVFRASAFRPSAG PGAPGDLEAQDAGGSTPEPTTPGPQDTQARAPSTRPAGRETVPWPNTPVEDDEMTPQETPPVHP TRTRRLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
ACCN | NC_001798 |
ORF Size | 3342 bp |
OTI Disclaimer | The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NC_001798.1, NP_044507 |
RefSeq ORF | 3342 bp |
Locus ID | 1487323 |
MW | 118.8 kDa |
Documents
Product Manuals |
FAQs |
SDS |
Resources
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