UL37 (NC_001798) Virus Tagged ORF Clone

CAT#: VC100902

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for tegument protein UL37 [Human herpesvirus 2], codon optimized for human cell expression, NP_044507



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CNY 16,820.00


货期*
6周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 100 ul

CNY 600.00


Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
    • 100 ul

CNY 1,050.00

Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC100902 represents NCBI reference of NP_044507 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCTGACAGTGCTCTGCAGGTGCCCGCCCCCGCCGGCATGACTCCACCATCTGCACCTCCACCTAACG
GCCCTCTCCAGGTTCTGCTTGGGTCTCTTACAAATCTGAGGCGACCTCCAAGTCCTTCTAGTGAGCCAGC
GGGGAGTGCTGATGAGCCTGCGTTTCTCTCAGCTGCTAAGCTCCATGCCGCTACAGCTGCCTTCCTCCTG
TCAGGCGCCGCCGTCGGACCAGCCGAGGCACGAGCATGCTGGCATCCACTGCTGGAACAGCTGTGCGCTC
TGCATAGAGCTCACGGGCTTCCTGAAACGGCACTGTTGGCCGAAAATCTGCCGGGTTTGCTGGTGCACCG
CATGGCAGTGGCTCTGCCCGAGACTCCCGAAGCCGCTTTCAGAGAAATGGATGTCATCAAGGACACCGTC
TTGGCCATTACAGGCTCAGACACTACTCACGCCTTGGAGGCGGCCGGCCTCAGGACCACCGCCGCCCTGG
GTCCCGTTAGAGTCAGGCAGTGCGCTGTGGAGTGGATCGATAGGTGGAGGACAGTTACACAGTCCTGTTT
GGCAATGAACCCACGCACATCACTCGAAGCTCTGGGCGAGATGTCTCTGAAAATGTCACCCGTCCCTCTG
GGTCAGCCTGGCGCTAATCTGACTACCCCGGCATACTCTCTGCTCTTCCCTTCACCAATTGTGCAAGAAG
GACTCCGATTCCTGGCCCTGGTTAGCAACTGGGTTACACTGTTTAGCGCACACTTGCAGCGAATTGACGA
TGCAGCCCTGACCCCACTCACACGCGCCTTGTTTACACTGGCCCTGGTCGACGACTATCTCACAACACCG
GATAGAGGCGCCGTGGTACCTCCACCTCTGCTGGCCCAGTTTCAGCATACCGTCCGAGAGATCGATCCCG
CAATTATGATTCCTCCCCTGGAGGCAACGAAGATGGTGCGCTCTCGGGAGGAGGTGCGGGTGTCAACTGC
CTTGAGTAGAGTCAGTCCACGGTCTGCTTGCGCCCCGCCTGGAACGCTCATGGCTCGCGTCAGAACTGAT
GCCGCCGTGTTTGACCCTGACGTTCCATTTCTCTCAGCTTCCGCCCTTGCCATATTCAGACCCGCTGTGA
CAGGATTGCTCCAGCTGGGAGAACCGCCGTCAGCCGGCGCCCAGCAGCGGCTGTTGGCGCTCCTTCAGCA
GACCTGGGCTCTGGTACAGAACAGCAATTCTCCCAGCGTGGTTATCAACACCTTGACAGACGCTGGGTTT
ACACCAGCCCATTGTACGCAATATATTAGTGCGTTGGAGGGCTTCCTGGTTGCCGGAGTCCCTGCCAGGA
CCCCCCCCGGTCACGGGCTCTCCGAGATTCAGCAACTGTTTGGATGCATCGCCCTGGCCGGAGCAAACGT
CTTTGGACTCGCCCGGGAATACGGACATTACGCAGGCTACGTGAAAACCTTTCGAAGGATACAGGGTGCC
TCCGAGCACACACACGGCAGACTGTGCGAAGCTGTCGGACTTTCAGGCGGGGTGCTGAGCCAGACTCTGG
CTCGAATTATGGGACCTGCCGTGCCAACCGAACATCTGGCCAGTTTGAGGAGGACCCTTGTAGGAGAATT
CGAAACTGCCGAGAGAAGGTTCAGCGCGGGGCAGCCAAGTCTTCTGAGAGAGACCGCCCTGATATGGCTC
GATGTTTACGGCCAGACTCACTGGGATTTGACTCCTACCACACCTGCCACACCCCTCTCCGCACTTCTGC
CGGTGGGGCCACCTAGTCACGCCCCTAGCGTCCATTTGGCAGCCGCCACTAAGATACGGTTCCCCGCACT
GGAAGGGATCCACCCAAACGTCCTGGCCGATCCTGGTTTTGTTCCATATGTCCTGGCCCTTGTAGTGGGT
GATGCTCTGCGGGCCACATGCAACGCCGCTTATCTGCCAAGACCTATTGAGTTCGCCCTGCGGGTACTGG
CTTGGGCGAGGGATTTCGGCCTTGGATACCTCCCAACAGTCGAGGGCCACAGGACAAAACTTGGCGCCCT
GATCACCCTCCTGGAGCCCGCAACCCGGGCCGGCGTGGGGCCAACTATGCAAATGGCCGACAACATCGAG
CAGTTGCTGAGGGAGCTGTATGTTATCGCTAGAGGGGCTGTGGAGCAACTGAGACCAGCTGTGCAACTCC
CACCTCCTCAGCCGCCCGAGGTGGGAAGTAGTCTCCTTCTGATCTCCATGTACGCCCTGGCCGCGCGGGG
AGTTTTGCAGGAGTTGGCTGAGCGGGCTGATCCCCTCGTAAGACAATTGGAGGATGCGATCGTCCTTCTT
AGGCTGCACATGCGAACACTTGCTGCATTCTTCGAATGCCGGTTTGAGTCTGACGGGCATCGACTCTACG
CGGTGGTTGCCGATGCTCATGAGCGGTTGGGACCTTGGCGCCCGGAGGCTATGGGGGATGCCGTCTCACA
GTATTGTGGCATGTATCACGACGCCAAGCGAGCTCTGGTCGCTAGTCTCGCCGGCCTGCGGAGCGTGGTG
ACAGAGACAACAGCTCACCTCGGGGTGTGCGACGAGCTGGCCGCCCAAGTGTCACACGAGGGAAATGTCC
TGGCAGTGGTTCGGCGAGAAATCCACGGTTTTCTGGCCATCGTGTCAGGTATCCATGCCCGCGCAAGCAA
GTTGATGAGCGGAGACCAGGTACCTGGATTTTGCTACATGTCCCAATTTCTGGCCAGGTGGAGACGACTC
AGTGCTGGGTATCAGGCAGCCCGGGCGGCCACCGGCCCCGAGCGGGTAGCCGAGTTCGTGCAGGAGTTGC
ACGATACTTGGAAGGGACTGCAGACCGAGCGAGCACTGGTTGTCGCCAGATTTGCCTCAAGCGCAGACCA
GAGAACAGCCGCCATCCAAGAGGTCATGGCACACGCAACAGAGGACGCACCTCCAAGTCCCGCCGCTGAT
CTTGTCGTGTTGACCAACCGGCACGATTTGGGAGCCTGGGGCGATTATAGTCTGGGTCCCCTGGGGCAGC
CTACGGTGGTGCCTGACAGCGTGGATCTTAGCCCCCAAGGACTCGCTGCCACCCTTTCCATGGACTGGTT
GTTGATCAATGAGCTCCTCCAGGTTACGGACGGTGTCTTCAGGGCCAGTGCTTTTCGACCATCCGCTGGC
CCAGGAGCGCCTGGCGATCTCGAAGCTCAAGATGCAGGCGGGTCCACTCCCGAACCTACAACACCTGGCC
CCCAGGATACCCAAGCAAGGGCACCATCTACCAGACCCGCCGGGAGAGAAACTGTGCCATGGCCTAACAC
CCCCGTAGAGGATGACGAGATGACACCCCAGGAGACCCCACCCGTCCACCCC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC100902 representing NP_044507
Red=Cloning sites Green=Tags

MSDSALQVPAPAGMTPPSAPPPNGPLQVLLGSLTNLRRPPSPSSEPAGSADEPAFLSAAKLHAATAAFLL
SGAAVGPAEARACWHPLLEQLCALHRAHGLPETALLAENLPGLLVHRMAVALPETPEAAFREMDVIKDTV
LAITGSDTTHALEAAGLRTTAALGPVRVRQCAVEWIDRWRTVTQSCLAMNPRTSLEALGEMSLKMSPVPL
GQPGANLTTPAYSLLFPSPIVQEGLRFLALVSNWVTLFSAHLQRIDDAALTPLTRALFTLALVDDYLTTP
DRGAVVPPPLLAQFQHTVREIDPAIMIPPLEATKMVRSREEVRVSTALSRVSPRSACAPPGTLMARVRTD
AAVFDPDVPFLSASALAIFRPAVTGLLQLGEPPSAGAQQRLLALLQQTWALVQNSNSPSVVINTLTDAGF
TPAHCTQYISALEGFLVAGVPARTPPGHGLSEIQQLFGCIALAGANVFGLAREYGHYAGYVKTFRRIQGA
SEHTHGRLCEAVGLSGGVLSQTLARIMGPAVPTEHLASLRRTLVGEFETAERRFSAGQPSLLRETALIWL
DVYGQTHWDLTPTTPATPLSALLPVGPPSHAPSVHLAAATKIRFPALEGIHPNVLADPGFVPYVLALVVG
DALRATCNAAYLPRPIEFALRVLAWARDFGLGYLPTVEGHRTKLGALITLLEPATRAGVGPTMQMADNIE
QLLRELYVIARGAVEQLRPAVQLPPPQPPEVGSSLLLISMYALAARGVLQELAERADPLVRQLEDAIVLL
RLHMRTLAAFFECRFESDGHRLYAVVADAHERLGPWRPEAMGDAVSQYCGMYHDAKRALVASLAGLRSVV
TETTAHLGVCDELAAQVSHEGNVLAVVRREIHGFLAIVSGIHARASKLMSGDQVPGFCYMSQFLARWRRL
SAGYQAARAATGPERVAEFVQELHDTWKGLQTERALVVARFASSADQRTAAIQEVMAHATEDAPPSPAAD
LVVLTNRHDLGAWGDYSLGPLGQPTVVPDSVDLSPQGLAATLSMDWLLINELLQVTDGVFRASAFRPSAG
PGAPGDLEAQDAGGSTPEPTTPGPQDTQARAPSTRPAGRETVPWPNTPVEDDEMTPQETPPVHP

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NC_001798
ORF Size 3342 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NC_001798.1, NP_044507
RefSeq ORF 3342 bp
Locus ID 1487323
MW 118.8 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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