UL52 (NC_001798) Virus Tagged ORF Clone

CAT#: VC100918

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for helicase-primase primase subunit [Human herpesvirus 2], codon optimized for human cell expression, NP_044523



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CNY 16,150.00


货期*
7周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 100 ul

CNY 600.00


Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
    • 100 ul

CNY 1,050.00

Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC100918 represents NCBI reference of NP_044523 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGGACAGAGGATTGTGACCACGAAGGACGCAGCGTCGCAGCCCCCGTCGAAGTCACAGCCCTGTATG
CGACCGATGGCTGTGTTATCACTAGCAGCCTGGCACTGCTGACAAATTGTTTGCTTGGGGCAGAACCACT
GTATATCTTCTCATATGACGCATATCGCAGCGATGCCCCCAACGGTCCTACAGGGGCTCCAACCGAGCAG
GAAAGGTTCGAGGGGTCTCGGGCTCTCTATAGGGATGCCGGTGGTCTGAACGGTGATTCTTTCAGAGTGA
CATTCTGCCTCCTGGGAACCGAAGTAGGCGTCACCCACCACCCAAAGGGGCGGACAAGACCCATGTTCGT
CTGCAGATTTGAGAGGGCAGATGACGTCGCTGTGCTGCAGGATGCGTTGGGCAGGGGGACCCCCCTGCTG
CCAGCTCATGTGACTGCAACCCTCGATCTGGAGGCAACCTTTGCTCTGCACGCAAATATTATAATGGCAC
TGACAGTGGCTATTGTGCACAACGCTCCTGCACGCATTGGTTCTGGCTCCACAGCTCCTTTGTACGAACC
TGGAGAGAGTATGCGATCTGTGGTCGGACGAATGAGCCTTGGACAGCGGGGACTGACCACACTCTTCGTG
CACCACGAAGCCCGGGTCCTCGGCGCATACCGCCGGGCATATTATGGAAGTGCACAGAGCCCGTTTTGGT
TTCTGTCCAAGTTTGGCCCGGATGAGAAGAGCCTCGTTCTCGCTGCACGATACTATCTGTTGCAGGCGCC
TCGACTCGGCGGCGCAGGGGCCACCTATGACTTGCAGGCTGTCAAGGATATCTGTGCCACTTATGCTATA
CCCCACGATCCCCGACCTGACACTCTCTCAGCAGCTAGTCTGACGAGTTTTGCTGCGATTACCAGGTTCT
GCTGCACCTCCCAGTATTCAAGAGGGGCTGCCGCGGCAGGCTTCCCCTTGTATGTCGAAAGAAGGATCGC
CGCTGACGTTAGGGAGACTGGTGCCCTCGAGAAGTTCATCGCGCATGACAGAAGCTGCTTGCGAGTGAGT
GATAGGGAGTTTATTACTTATATATACTTGGCACATTTTGAGTGCTTTAGTCCCCCAAGACTGGCAACCC
ACCTGCGGGCGGTGACCACACATGATCCTAGTCCTGCTGCAAGTACAGAGCAGCCATCCCCCTTGGGACG
GGAGGCCGTTGAGCAGTTTTTTCGGCATGTACGAGCCCAGCTCAACATTCGCGAGTATGTTAAGCAAAAT
GTGACACCTCGCGAAACGGCACTGGCCGGAGACGCCGCCGCTGCCTACCTGAGGGCCAGAACATATGCTC
CAGCCGCTCTGACCCCTGCACCTGCCTATTGTGGGGTGGCCGATTCCTCTACAAAAATGATGGGGAGGCT
GGCTGAAGCCGAGAGACTTCTGGTGCCTCATGGGTGGCCTGCATTCGCTCCTACTACCCCAGGTGATGAC
GCCGGTGGCGGGACAGCTGCACCTCAGACATGCGGGATCGTTAAAAGGCTCCTGAAGTTGGCAGCCACAG
AGCAACAGGGTACTACCCCCCCAGCTATTGCAGCGCTGATGCAGGACGCATCAGTGCAAACACCTCTGCC
AGTATACAGAATTACCATGAGTCCAACTGGGCAGGCTTTCGCTGCCGCAGCCAGAGACGACTGGGCTAGG
GTGACAAGAGATGCAAGACCCCCGGAAGCCACGGTCGTCGCAGATGCTGCTGCCGCCCCAGAGCCAGGAG
CACTGGGAAGACGCTTGACGCGGAGAATTTGTGCACGGGGACCTGCCCTCCCTCCAGGGGGTCTTGCCGT
AGGAGGCCAGATGTACGTTAACAGAAATGAGATTTTCAATGCCGCGCTGGCAGTAACTAACATTATCCTG
GATTTGGATATCGCACTGAAGGAGCCCGTGCCTTTTCCTCGCCTGCACGAAGCCCTCGGCCATTTTCGCA
GAGGTGCCCTCGCCGCCGTACAACTTTTGTTCCCAGCAGCTCGGGTGGATCCTGACGCCTATCCATGTTA
TTTCTTCAAGAGTGCGTGCAGGCCTAGAGCTCCCCCAGTTTGTGCTGGGGACGGTCCGTCCGCCGGTGGA
GATGATGGTGATGGGGATTGGTTCCCAGATGCAGGCGGTCCTGGAGACGAGGAATGGGAAGAGGACACAG
ACCCCATGGATACCACTCACGGCCCCCTTCCCGATGATGAAGCCGCCTATCTCGACTTGTTGCATGAGCA
GATTCCCGCCGCCACCCCTTCCGAGCCAGACAGTGTGGTGTGCTCATGCGCCGACAAAATCGGCCTCCGA
GTATGCTTGCCCGTGCCCGCACCCTATGTTGTTCACGGGTCCCTGACCATGCGAGGGGTTGCGCGGGTAA
TTCAGCAGGCAGTGCTCCTGGATCGAGATTTTGTAGAAGCCGTGGGCTCCCATGTCAAAAACTTCCTCCT
CATCGACACAGGGGTGTATGCCCACGGTCATAGCTTGAGACTGCCGTACTTCGCCAAGATCGGTCCCGAT
GGTAGTGCATGTGGGCGACTCCTGCCCGTGTTCGTCATTCCCCCTGCCTGCGAGGATGTTCCAGCATTTG
TTGCTGCCCATGCAGATCCCAGGAGATTTCACTTTCACGCCCCACCTATGTTTTCAGCTGCGCCCAGGGA
GATCAGGGTGCTCCATTCACTCGGCGGGGATTATGTCTCCTTCTTTGAAAAGAAGGCCAGCCGGAACGCT
CTGGAGCACTTTGGAAGGCGCGAGACCCTCACAGAGGTCCTGGGCAGGTACGATGTGCGGCCAGATGCCG
GAGAAACGGTGGAAGGCTTTGCTAGCGAACTTTTGGGCAGAATTGTGGCATGTATAGAGGCCCACTTCCC
TGAACACGCCAGAGAATACCAAGCAGTTTCCGTCAGACGCGCAGTAATCAAGGACGATTGGGTTTTGCTC
CAGCTGATCCCCGGTCGAGGCGCCTTGAACCAGTCTCTGTCCTGTCTCAGGTTTAAACATGGTCGAGCTA
GTAGAGCTACAGCCCGAACATTTCTGGCCTTGTCCGTAGGCACAAATAACAGGCTGTGTGCATCCTTGTG
CCAGCAATGTTTCGCCACCAAGTGCGATAATAACAGATTGCACACGCTCTTCACAGTGGACGCCGGCACC
CCTTGCTCTCGGTCCGCCCCCTCCTCTACCTCACGCCCCTCCTCTAGC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC100918 representing NP_044523
Red=Cloning sites Green=Tags

MGTEDCDHEGRSVAAPVEVTALYATDGCVITSSLALLTNCLLGAEPLYIFSYDAYRSDAPNGPTGAPTEQ
ERFEGSRALYRDAGGLNGDSFRVTFCLLGTEVGVTHHPKGRTRPMFVCRFERADDVAVLQDALGRGTPLL
PAHVTATLDLEATFALHANIIMALTVAIVHNAPARIGSGSTAPLYEPGESMRSVVGRMSLGQRGLTTLFV
HHEARVLGAYRRAYYGSAQSPFWFLSKFGPDEKSLVLAARYYLLQAPRLGGAGATYDLQAVKDICATYAI
PHDPRPDTLSAASLTSFAAITRFCCTSQYSRGAAAAGFPLYVERRIAADVRETGALEKFIAHDRSCLRVS
DREFITYIYLAHFECFSPPRLATHLRAVTTHDPSPAASTEQPSPLGREAVEQFFRHVRAQLNIREYVKQN
VTPRETALAGDAAAAYLRARTYAPAALTPAPAYCGVADSSTKMMGRLAEAERLLVPHGWPAFAPTTPGDD
AGGGTAAPQTCGIVKRLLKLAATEQQGTTPPAIAALMQDASVQTPLPVYRITMSPTGQAFAAAARDDWAR
VTRDARPPEATVVADAAAAPEPGALGRRLTRRICARGPALPPGGLAVGGQMYVNRNEIFNAALAVTNIIL
DLDIALKEPVPFPRLHEALGHFRRGALAAVQLLFPAARVDPDAYPCYFFKSACRPRAPPVCAGDGPSAGG
DDGDGDWFPDAGGPGDEEWEEDTDPMDTTHGPLPDDEAAYLDLLHEQIPAATPSEPDSVVCSCADKIGLR
VCLPVPAPYVVHGSLTMRGVARVIQQAVLLDRDFVEAVGSHVKNFLLIDTGVYAHGHSLRLPYFAKIGPD
GSACGRLLPVFVIPPACEDVPAFVAAHADPRRFHFHAPPMFSAAPREIRVLHSLGGDYVSFFEKKASRNA
LEHFGRRETLTEVLGRYDVRPDAGETVEGFASELLGRIVACIEAHFPEHAREYQAVSVRRAVIKDDWVLL
QLIPGRGALNQSLSCLRFKHGRASRATARTFLALSVGTNNRLCASLCQQCFATKCDNNRLHTLFTVDAGT
PCSRSAPSSTSRPSSS

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NC_001798
ORF Size 3198 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NC_001798.1, NP_044523
RefSeq ORF 3198 bp
Locus ID 1487341
MW 114.7 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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