UL46 (NC_001798) Virus Tagged ORF Clone

CAT#: VC100911

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for tegument protein VP11/12 [Human herpesvirus 2], codon optimized for human cell expression, NP_044516



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CNY 10,830.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 100 ul

CNY 600.00


Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
    • 100 ul

CNY 1,050.00

Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC100911 represents NCBI reference of NP_044516 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCAGCGGCGGGCCAGAGGCGCTTCCAGCCTGAGACTCGCTAGATGCCTCACCCCTGCCAACCTGATCA
GAGGGGCAAATGCCGGGGTCCCCGAACGGAGAATTTTCGCAGGGTGCCTCCTCCCTACTCCCGAAGGGCT
CCTCTCCGCTGCAGTGGGCGTGCTCCGGCAGCGGGCCGATGACCTGCAGCCAGCCTTCCTCACCGGCGCT
GACAGAAGCGTGCGGCTCGCTGCCCGGCATCACAACACCGTCCCTGAGTCCCTGATCGTGGACGGCCTCG
CCAGCGATCCCCACTATGACTACATCCGGCACTACGCCAGCGCCGCCAAGCAGGCACTCGGCGAGGTCGA
GCTGAGCGGCGGCCAACTGAGCCGGGCAATTCTCGCCCAGTACTGGAAATACCTCCAGACCGTCGTGCCC
AGCGGGCTCGACATTCCTGACGACCCCGCCGGCGACTGCGACCCCTCCCTGCACGTGCTGCTCCGGCCCA
CCCTGCTGCCCAAGCTCCTGGTGCGGGCCCCCTTCAAGTCCGGGGCCGCCGCCGCCAAGTACGCCGCCGC
CGTGGCCGGCCTGCGGGACGCCGCCCACCGGCTGCAGCAATATATGTTCTTCATGCGGCCCGCCGACCCA
TCCCGGCCCAGCACCGACACCGCCCTGCGGCTGTCCGAGCTCCTCGCATACGTGAGCGTCCTCTACCACT
GGGCCAGCTGGATGCTGTGGACCGCCGACAAGTACGTGTGTCGGCGGCTCGGCCCTGCCGACCGGCGGTT
CGTCGCCCTGTCCGGCAGCCTGGAGGCCCCCGCCGAAACCTTCGCCCGGCACCTCGACCGGGGGCCCTCC
GGGACCACCGGGAGCATGCAGTGCATGGCCCTCCGGGCCGCCGTGAGCGATGTGCTGGGCCACCTCACCC
GGCTCGCCCACCTCTGGGAAACCGGCAAGCGGAGCGGGGGCACCTACGGCATCGTGGACGCTATCGTCAG
CACCGTCGAGGTCCTCTCCATCGTGCACCACCACGCCCAGTACATCATCAACGCAACCCTGACCGGGTAC
GTGGTGTGGGCAAGCGACAGCCTGAACAACGAGTATCTGACTGCCGCCGTGGACAGCCAGGAACGGTTCT
GCCGGACCGCTGCCCCCCTCTTCCCCACCATGACCGCCCCCTCCTGGGCACGGATGGAGCTGAGCATCAA
GAGCTGGTTCGGCGCCGCCCTCGCCCCCGACCTCCTGCGGTCCGGCACACCCAGCCCCCACTATGAGAGC
ATCCTCCGGCTCGCCGCAAGCGGCCCTCCCGGCGGCCGGGGCGCCGTGGGCGGCTCCTGTCGGGATAAAA
TCCAGCGGACCCGGCGGGACAACGCCCCTCCTCCACTGCCCCGGGCCCGGCCTCACTCCACACCTGCCGC
CCCCCGGCGGTGCAGACGGCACCGGGAGGACCTGCCCGAGCCCCCCCACGTCGACGCAGCCGATCGGGGC
CCCGAGCCCTGCGCCGGGCGGCCCGCCACCTACTACACCCACATGGCCGGCGCCCCCCCCAGACTGCCAC
CCCGGAACCCCGCTCCCCCCGAGCAGCGGCCCGCTGCCGCAGCCCGGCCCCTGGCCGCCCAGCGGGAAGC
CGCAGGCGTCTACGACGCTGTGCGGACCTGGGGCCCCGACGCCGAGGCTGAGCCCGACCAAATGGAGAAC
ACCTACCTGCTGCCCGACGACGACGCTGCAATGCCCGCAGGCGTGGGCCTGGGAGCCACACCCGCTGCCG
ACACCACCGCCGCCGCCGCCTGGCCCGCAGAGAGCCACGCCCCCCGGGCACCCTCCGAGGACGCCGACAG
CATTTACGAAAGCGTGGGCGAGGACGGCGGACGGGTGTACGAGGAGATCCCCTGGGTGCGGGTCTACGAG
AACATTTGCCCCCGGCGGCGGCTGGCCGGGGGCGCCGCACTGCCTGGCGACGCTCCCGACTCCCCATACA
TCGAGGCCGAGAACCCCCTGTACGACTGGGGCGGCAGCGCCCTCTTTAGCCCACGGCGGGCCACCCGGGC
TCCAGACCCCGGGCTCAGCCTCTCCCCCATGCCCGCTCGGCCTCGGACAAACGCTCTGGCAAACGACGGC
CCCACAAACGTGGCCGCTCTGTCCGCTCTCCTGACCAAACTCAAGCGGGGCCGGCACCAGTCCCAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC100911 representing NP_044516
Red=Cloning sites Green=Tags

MQRRARGASSLRLARCLTPANLIRGANAGVPERRIFAGCLLPTPEGLLSAAVGVLRQRADDLQPAFLTGA
DRSVRLAARHHNTVPESLIVDGLASDPHYDYIRHYASAAKQALGEVELSGGQLSRAILAQYWKYLQTVVP
SGLDIPDDPAGDCDPSLHVLLRPTLLPKLLVRAPFKSGAAAAKYAAAVAGLRDAAHRLQQYMFFMRPADP
SRPSTDTALRLSELLAYVSVLYHWASWMLWTADKYVCRRLGPADRRFVALSGSLEAPAETFARHLDRGPS
GTTGSMQCMALRAAVSDVLGHLTRLAHLWETGKRSGGTYGIVDAIVSTVEVLSIVHHHAQYIINATLTGY
VVWASDSLNNEYLTAAVDSQERFCRTAAPLFPTMTAPSWARMELSIKSWFGAALAPDLLRSGTPSPHYES
ILRLAASGPPGGRGAVGGSCRDKIQRTRRDNAPPPLPRARPHSTPAAPRRCRRHREDLPEPPHVDAADRG
PEPCAGRPATYYTHMAGAPPRLPPRNPAPPEQRPAAAARPLAAQREAAGVYDAVRTWGPDAEAEPDQMEN
TYLLPDDDAAMPAGVGLGATPAADTTAAAAWPAESHAPRAPSEDADSIYESVGEDGGRVYEEIPWVRVYE
NICPRRRLAGGAALPGDAPDSPYIEAENPLYDWGGSALFSPRRATRAPDPGLSLSPMPARPRTNALANDG
PTNVAALSALLTKLKRGRHQSH

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NC_001798
ORF Size 2166 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NC_001798.1, NP_044516
RefSeq ORF 2166 bp
Locus ID 1487333
MW 77.7 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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