UL19 (NC_001798) Virus Tagged ORF Clone

CAT#: VC100883

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for major capsid protein [Human herpesvirus 2], codon optimized for human cell expression, NP_044488



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CNY 20,710.00


货期*
8周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 100 ul

CNY 600.00


Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
    • 100 ul

CNY 1,050.00

Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC100883 represents NCBI reference of NP_044488 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCAGCTCCTGCAAGAGACCCGCCCGGCTATCGGTACGCAGCGGCTATCCTGCCCACCGGCTCCATTC
TGTCAACTATTGAGGTCGCTTCCCATCGCCGACTTTTCGACTTCTTTGCTGCAGTGAGGAGCGACGAAAA
CTCTCTTTATGATGTCGAATTTGATGCACTGCTGGGTTCCTACTGCAACACTCTGTCCCTGGTGCGCTTT
TTGGAGCTGGGCCTTTCCGTTGCTTGTGTCTGTACTAAATTTCCTGAACTGGCATATATGAACGAGGGCA
GGGTGCAATTTGAAGTCCATCAGCCACTTATAGCCCGCGACGGGCCGCACCCAGTTGAACAACCCGTTCA
TAACTACATGACCAAGGTCATAGACCGAAGAGCTCTTAATGCCGCCTTTTCTCTGGCAACAGAGGCGATA
GCCCTCCTGACAGGTGAAGCTCTCGACGGTACTGGAATCTCTCTGCACCGGCAGCTGAGGGCCATCCAAC
AGCTGGCCAGAAACGTCCAGGCAGTTCTCGGGGCATTTGAGAGAGGAACGGCCGATCAAATGCTTCATGT
CCTTCTTGAGAAGGCTCCCCCGCTGGCTCTGCTCCTGCCCATGCAACGCTACCTGGATAATGGAAGGCTT
GCCACACGGGTCGCACGCGCAACCCTGGTCGCCGAGCTGAAACGGTCCTTTTGCGACACATCCTTCTTCC
TGGGCAAAGCAGGGCACAGACGGGAAGCTATTGAGGCGTGGCTCGTCGACCTGACTACCGCTACCCAGCC
AAGCGTAGCAGTCCCTCGCCTCACACATGCAGATACCAGGGGCCGCCCAGTGGACGGGGTGCTCGTGACT
ACCGCAGCAATTAAGCAGCGGCTCCTCCAGAGCTTTCTGAAAGTCGAGGATACCGAAGCGGACGTGCCAG
TGACTTATGGCGAGATGGTACTCAATGGAGCAAACCTTGTTACAGCCCTCGTGATGGGGAAGGCAGTTAG
GAGTCTGGACGATGTGGGCAGGCACTTGCTCGACATGCAGGAAGAACAGCTGGAAGCCAACAGAGAAACC
CTTGATGAGTTGGAATCTGCCCCCCAAACGACTAGGGTGAGAGCTGACCTCGTGGCTATCGGAGATCGCC
TGGTGTTCCTGGAGGCACTGGAGCGAAGGATTTATGCTGCCACAAATGTCCCCTATCCTCTGGTGGGAGC
CATGGATCTTACCTTCGTGCTGCCTCTCGGCCTGTTCAACCCAGCAATGGAAAGGTTCGCAGCTCACGCT
GGAGACCTGGTGCCGGCACCCGGCCATCCTGAACCCAGAGCGTTCCCACCCCGGCAGCTCTTTTTCTGGG
GCAAGGACCATCAAGTGCTGCGGTTGTCCATGGAAAATGCCGTGGGGACCGTGTGCCATCCGTCTCTTAT
GAACATTGACGCGGCTGTGGGAGGTGTTAACCATGACCCAGTGGAGGCCGCCAACCCGTATGGAGCATAT
GTAGCAGCCCCCGCCGGGCCTGGGGCCGACATGCAGCAGCGGTTCCTCAATGCATGGAGGCAGAGGCTGG
CCCATGGACGAGTGAGATGGGTAGCCGAATGCCAAATGACAGCTGAACAGTTCATGCAGCCAGATAACGC
CAATCTCGCTCTGGAACTGCATCCCGCTTTCGACTTCTTCGCCGGTGTCGCGGACGTAGAGCTCCCTGGC
GGTGAAGTGCCTCCTGCAGGACCCGGGGCCATACAGGCCACTTGGCGGGTCGTGAACGGAAACCTGCCTC
TCGCCCTTTGTCCCGTAGCCTTCCGGGACGCTCGGGGTCTGGAACTGGGCGTAGGTAGGCACGCCATGGC
CCCAGCCACTATCGCAGCGGTTCGCGGGGCATTTGAGGATCGGTCCTATCCCGCTGTCTTTTACTTGCTG
CAAGCTGCTATCCACGGAAACGAACACGTATTCTGCGCGCTTGCAAGGCTGGTGACTCAATGTATCACAA
GTTATTGGAACAATACCCGCTGTGCAGCTTTCGTGAACGATTACTCCTTGGTCTCATACATCGTAACGTA
CCTGGGTGGAGATTTGCCCGAAGAGTGCATGGCTGTCTACCGCGATCTGGTCGCCCACGTGGAAGCCCTG
GCCCAGCTCGTGGATGATTTTACCCTGCCTGGCCCTGAACTGGGAGGACAGGCTCAGGCCGAACTCAATC
ACCTGATGAGAGATCCTGCCCTCCTCCCTCCCCTTGTGTGGGATTGTGATGGGCTGATGCGGCACGCTGC
TCTCGACCGGCATCGCGACTGCCGGATCGACGCGGGAGGACACGAACCTGTGTATGCCGCTGCTTGTAAC
GTCGCTACCGCTGACTTCAATCGCAACGATGGCAGACTTTTGCACAACACTCAGGCCCGAGCTGCTGATG
CCGCCGATGACCGCCCTCATAGACCAGCTGACTGGACAGTTCATCATAAAATCTATTATTACGTTCTGGT
TCCTGCCTTCAGTCGCGGACGCTGTTGCACCGCCGGCGTCAGATTCGACCGCGTTTACGCTACCCTGCAG
AATATGGTAGTCCCAGAAATCGCCCCAGGCGAGGAATGTCCATCTGACCCAGTGACCGACCCCGCCCATC
CACTGCACCCAGCCAATCTCGTCGCTAACACCGTGAAAAGGATGTTTCACAATGGTAGGGTAGTGGTTGA
CGGCCCAGCCATGTTGACCCTGCAGGTGCTCGCCCATAACATGGCTGAAAGAACGACCGCCCTGCTTTGC
TCCGCTGCCCCAGATGCAGGCGCAAACACCGCCAGCACTGCCAATATGAGGATCTTCGATGGCGCGTTGC
ACGCGGGGGTGCTGCTGATGGCACCACAGCATTTGGACCATACCATTCAGAACGGGGAATACTTCTACGT
GTTGCCGGTGCACGCTCTCTTCGCTGGGGCAGACCATGTGGCCAACGCACCCAATTTCCCACCAGCCCTG
AGAGATCTTGCTCGCGACGTCCCTCTTGTTCCTCCCGCTTTGGGTGCCAACTATTTCAGCTCCATTAGAC
AGCCGGTCGTCCAACACGCACGGGAGAGTGCCGCCGGAGAAAACGCATTGACTTACGCCCTCATGGCCGG
CTACTTCAAGATGTCCCCTGTGGCATTGTATCATCAGTTGAAGACTGGCCTGCATCCGGGGTTTGGATTC
ACAGTTGTGAGGCAAGACCGGTTCGTAACCGAGAACGTGCTTTTCAGCGAGCGGGCTAGTGAAGCCTACT
TTCTCGGTCAGCTGCAGGTGGCCCGCCACGAGACAGGTGGTGGTGTAAACTTCACTCTCACTCAGCCACG
GGGGAATGTGGATCTGGGAGTGGGGTACACCGCCGTGGCCGCTACTGGGACTGTTCGGAACCCTGTTACC
GACATGGGCAACCTGCCTCAGAACTTCTACCTGGGCCGGGGTGCACCACCCCTGCTGGACAACGCAGCCG
CGGTATACCTGAGAAATGCAGTCGTAGCTGGCAACAGACTCGGACCAGCTCAGCCGTTGCCTGTGTTCGG
ATGTGCTCAGGTGCCAAGGAGGGCAGGCATGGACCATGGTCAGGATGCAGTTTGTGAATTCATTGCCACT
CCAGTTGCCACGGATATCAACTATTTTCGCAGGCCCTGCAATCCCCGGGGCCGAGCTGCCGGAGGCGTCT
ATGCCGGAGATAAGGAAGGCGACGTAATCGCACTGATGTACGACCACGGACAGAGTGATCCCGCACGCCC
ATTCGCAGCAACAGCTAATCCCTGGGCTAGCCAGAGATTTTCCTACGGTGACCTGCTGTATAACGGTGCC
TACCACCTGAATGGGGCATCCCCAGTGCTCAGCCCGTGTTTTAAGTTCTTCACAGCCGCAGACATTACCG
CCAAACATCGCTGTTTGGAACGGCTGATCGTAGAGACAGGCTCAGCGGTCTCTACCGCCACAGCTGCCAG
CGACGTTCAATTCAAAAGACCCCCCGGGTGTCGAGAACTGGTTGAGGATCCCTGTGGACTCTTTCAGGAG
GCCTATCCCATAACGTGCGCCTCCGACCCTGCCCTCCTCCGGTCTGCGAGGGACGGCGAGGCCCACGCCA
GGGAAACCCATTTCACCCAGTACCTGATATACGACGCTAGCCCACTGAAAGGTTTGTCCCTG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC100883 representing NP_044488
Red=Cloning sites Green=Tags

MAAPARDPPGYRYAAAILPTGSILSTIEVASHRRLFDFFAAVRSDENSLYDVEFDALLGSYCNTLSLVRF
LELGLSVACVCTKFPELAYMNEGRVQFEVHQPLIARDGPHPVEQPVHNYMTKVIDRRALNAAFSLATEAI
ALLTGEALDGTGISLHRQLRAIQQLARNVQAVLGAFERGTADQMLHVLLEKAPPLALLLPMQRYLDNGRL
ATRVARATLVAELKRSFCDTSFFLGKAGHRREAIEAWLVDLTTATQPSVAVPRLTHADTRGRPVDGVLVT
TAAIKQRLLQSFLKVEDTEADVPVTYGEMVLNGANLVTALVMGKAVRSLDDVGRHLLDMQEEQLEANRET
LDELESAPQTTRVRADLVAIGDRLVFLEALERRIYAATNVPYPLVGAMDLTFVLPLGLFNPAMERFAAHA
GDLVPAPGHPEPRAFPPRQLFFWGKDHQVLRLSMENAVGTVCHPSLMNIDAAVGGVNHDPVEAANPYGAY
VAAPAGPGADMQQRFLNAWRQRLAHGRVRWVAECQMTAEQFMQPDNANLALELHPAFDFFAGVADVELPG
GEVPPAGPGAIQATWRVVNGNLPLALCPVAFRDARGLELGVGRHAMAPATIAAVRGAFEDRSYPAVFYLL
QAAIHGNEHVFCALARLVTQCITSYWNNTRCAAFVNDYSLVSYIVTYLGGDLPEECMAVYRDLVAHVEAL
AQLVDDFTLPGPELGGQAQAELNHLMRDPALLPPLVWDCDGLMRHAALDRHRDCRIDAGGHEPVYAAACN
VATADFNRNDGRLLHNTQARAADAADDRPHRPADWTVHHKIYYYVLVPAFSRGRCCTAGVRFDRVYATLQ
NMVVPEIAPGEECPSDPVTDPAHPLHPANLVANTVKRMFHNGRVVVDGPAMLTLQVLAHNMAERTTALLC
SAAPDAGANTASTANMRIFDGALHAGVLLMAPQHLDHTIQNGEYFYVLPVHALFAGADHVANAPNFPPAL
RDLARDVPLVPPALGANYFSSIRQPVVQHARESAAGENALTYALMAGYFKMSPVALYHQLKTGLHPGFGF
TVVRQDRFVTENVLFSERASEAYFLGQLQVARHETGGGVNFTLTQPRGNVDLGVGYTAVAATGTVRNPVT
DMGNLPQNFYLGRGAPPLLDNAAAVYLRNAVVAGNRLGPAQPLPVFGCAQVPRRAGMDHGQDAVCEFIAT
PVATDINYFRRPCNPRGRAAGGVYAGDKEGDVIALMYDHGQSDPARPFAATANPWASQRFSYGDLLYNGA
YHLNGASPVLSPCFKFFTAADITAKHRCLERLIVETGSAVSTATAASDVQFKRPPGCRELVEDPCGLFQE
AYPITCASDPALLRSARDGEAHARETHFTQYLIYDASPLKGLSL

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NC_001798
ORF Size 4122 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NC_001798.1, NP_044488
RefSeq ORF 4122 bp
Locus ID 1487302
MW 149.2 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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