UL40 (NC_001798) Virus Tagged ORF Clone
CAT#: VC100905
- TrueORF®
Myc-DDK-tagged ORF clone of viral ORF for ribonucleotide reductase subunit 2 [Human herpesvirus 2], codon optimized for human cell expression, NP_044510
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CNY 3,990.00
Product images
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CNY 600.00
CNY 1,050.00
Specifications
Product Data | |
Type | Virus Tagged ORF Clone |
Tag | Myc-DDK |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>The Viral ORF clone VC100905 represents NCBI reference of NP_044510 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s) GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACCCCGCTGTGAGCCCTGCAAGCACCGACCCTTTGGATACCCACGCCTCAGGAGCCGGTGCCGCCC CGATCCCAGTGTGCCCAACACCAGAGCGATACTTCTATACTTCCCAATGTCCCGACATAAACCATCTCCG ATCCCTGTCTATTCTGAACAGATGGCTGGAAACTGAACTGGTATTCGTTGGAGATGAGGAGGATGTGAGC AAGTTGAGCGAGGGAGAACTGGGTTTCTATAGGTTCCTCTTCGCTTTTCTGAGCGCAGCCGACGATCTCG TGACTGAGAACTTGGGCGGCTTGAGCGGCTTGTTCGAGCAAAAGGATATTCTCCACTACTACGTAGAGCA GGAGTGCATCGAGGTGGTGCATAGCAGAGTGTATAACATAATCCAACTCGTGCTGTTCCACAATAATGAC CAGGCTAGAAGGGCCTACGTGGCGAGAACAATCAATCATCCCGCTATAAGGGTGAAAGTCGATTGGCTGG AAGCTCGCGTACGCGAGTGCGACTCCATCCCTGAGAAGTTCATTCTGATGATACTGATTGAAGGCGTTTT TTTCGCCGCGAGCTTCGCCGCCATCGCATATCTCAGAACCAATAATCTGCTGAGGGTCACATGTCAATCC AATGACTTGATCAGCAGAGATGAAGCAGTTCATACGACCGCCTCCTGTTATATATATAACAATTATCTCG GCGGTCACGCAAAACCAGAGGCCGCAAGGGTTTACAGACTCTTCAGAGAGGCAGTGGATATAGAGATCGG ATTCATCAGATCTCAAGCCCCAACTGATTCCAGCATTCTGAGCCCAGGCGCCCTGGCAGCAATTGAGAAC TACGTGCGGTTTTCAGCAGACAGGCTGCTGGGACTGATTCATATGCAGCCTCTCTATAGTGCTCCCGCTC CCGACGCTTCTTTTCCTCTCTCACTGATGTCCACAGACAAGCATACTAATTTCTTCGAGTGCAGGTCAAC CAGCTACGCAGGAGCCGTCGTGAATGACCTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >VC100905 representing NP_044510
Red=Cloning sites Green=Tags MDPAVSPASTDPLDTHASGAGAAPIPVCPTPERYFYTSQCPDINHLRSLSILNRWLETELVFVGDEEDVS KLSEGELGFYRFLFAFLSAADDLVTENLGGLSGLFEQKDILHYYVEQECIEVVHSRVYNIIQLVLFHNND QARRAYVARTINHPAIRVKVDWLEARVRECDSIPEKFILMILIEGVFFAASFAAIAYLRTNNLLRVTCQS NDLISRDEAVHTTASCYIYNNYLGGHAKPEAARVYRLFREAVDIEIGFIRSQAPTDSSILSPGALAAIEN YVRFSADRLLGLIHMQPLYSAPAPDASFPLSLMSTDKHTNFFECRSTSYAGAVVNDL TRTRRLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
ACCN | NC_001798 |
ORF Size | 1011 bp |
OTI Disclaimer | The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NC_001798.1, NP_044510 |
RefSeq ORF | 1011 bp |
Locus ID | 1487327 |
MW | 37.6 kDa |
Documents
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FAQs |
SDS |
Resources
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